Lateral movements of membrane glycoproteins restricted by dynamic cytoplasmic barriers

Michael Edidin, Scot C. Kuo, Michael Sheetz

Research output: Contribution to journalArticle

209 Citations (Scopus)

Abstract

Cell membranes often are patchy, composed of lateral domains. These domains may be formed by barriers within or on either side of the membrane bilayer. Major histocompatibility complex (MHC) class 1 molecules that were either transmembrane- (H-2Db) or glycosylphosphatidylinositol (GPI)-anchored (Qa2) were labeled with antibody-coated gold particles and moved across the cell surface with a laser optical tweezers until they encountered a barrier, the barrier-free path length (BFP). At room temperature, the BFPs of Qa2 and H-2Db were 1.7 ± 0.2 and 0.6 ± 0.1 (micrometers ± SEM), respectively. Barriers persisted at 34°C, although the BFP for both MHC molecules was fivefold greater at 34°C than at 23°C. This indicates that barriers to lateral movement are primarily on the cytoplasmic half of the membrane and are dynamic.

Original languageEnglish (US)
Pages (from-to)1379-1382
Number of pages4
JournalScience
Volume254
Issue number5036
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

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Optical Tweezers
Membrane Glycoproteins
Major Histocompatibility Complex
Cell Membrane
Glycosylphosphatidylinositols
Gold
Temperature
Membranes
Antibodies
2-benzyl-3-formylpropanoic acid

ASJC Scopus subject areas

  • General

Cite this

Lateral movements of membrane glycoproteins restricted by dynamic cytoplasmic barriers. / Edidin, Michael; Kuo, Scot C.; Sheetz, Michael.

In: Science, Vol. 254, No. 5036, 01.01.1991, p. 1379-1382.

Research output: Contribution to journalArticle

Edidin, Michael ; Kuo, Scot C. ; Sheetz, Michael. / Lateral movements of membrane glycoproteins restricted by dynamic cytoplasmic barriers. In: Science. 1991 ; Vol. 254, No. 5036. pp. 1379-1382.
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