Latex agglutination test for detection of Candida antigen in patients with disseminated disease

L. O. Gentry, I. D. Wilkinson, A. S. Lea, M. F. Price

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

A latex agglutination test has been devised which allows detection of a circulating antigen in patients with systemic infection due to Candida albicans, Candida tropicalis and Candida parapsilosis. Latex is sensitized with serum from rabbits immunized with whole heat killed Candida albicans blastoconidia. The active component of this serum is IgG. Control latex, used to differentiate non-specific agglutination, is sensitized with the same dilution of serum from a rabbit without antibody to Candida species. Sera from a number of patient groups were tested. While none of the hundred normal controls had an antigen titer of ≥ 1:4, 30 of 33 patients with documented disseminated candida infection had antigen titers of 1:4 to 1:32. Two of the 33 gave false negative results, and one caused nonspecific agglutination. In all patients who recovered after antifungal therapy antigen levels returned to within the range found in normal controls.

Original languageEnglish (US)
Pages (from-to)122-128
Number of pages7
JournalEuropean Journal of Clinical Microbiology
Volume2
Issue number2
DOIs
StatePublished - Apr 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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