Layered Double Hydroxide Nanoparticles for Efficient Gene Delivery for Cancer Treatment

Sudipta Senapati, Tanmoy Sarkar, Parimal Das, Pralay Maiti

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The use of cationic polymer based gene delivery vectors has several limitations such as low transfection efficiency, high toxicity, and inactivation by serum. The present work provides an inorganic based nanocarrier for efficient gene delivery and a method for preparing the same through a facile coprecipitation technique. The vehicle showed high loading capacity of DNA and can release the loaded DNA in a controlled pH-responsive manner. The developed gene delivery vehicle offers remarkable protection against DNase I and also provides protection against thermal damage. This vehicle also demonstrated efficient cellular uptake performance. Transfection and expression of plasmid gene encoding GFP proteins is achieved successfully by this LDH based vehicle. More interestingly, the developed Li-Al LDH efficiently induces GFP-p53 mediated apoptosis in HeLa cells exclusively sparing the normal tissue cells like NIH-3T3. The study demonstrates the potential of the developed inorganic based nanocarrier as a promising nonviral gene vector for tumor treatment.

Original languageEnglish (US)
Pages (from-to)2544-2554
Number of pages11
JournalBioconjugate Chemistry
Volume30
Issue number10
DOIs
StatePublished - Oct 16 2019
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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