LC/QTOF profile and preliminary stability studies of an enriched flavonoid fraction of Cecropia pachystachya Trécul leaves with potential antidepressant-like activity

Caroline Flach Ortmann, Helena Mendes Abelaira, Gislaine Zilli Réus, Zuleide Maria Ignácio, Vitor Clasen Chaves, Talitha Caldas dos Santos, Pâmela de Carvalho, Anelise Scussel Carlessi, Livia Bruchchen, Lucineia G. Danielski, Simone Gonçalves Cardoso, Angela Machado de Campos, Fabricia Petronilho, Joyce Rebelo, Meline Oliveira dos Santos Morais, Francieli Vuolo, Felipe Dal-Pizzol, Emilio Luiz Streck, João Quevedo, Flávio Henrique Reginatto

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

There is increasing interest in natural antioxidants that are candidates for the prevention of brain damage occurring in major depressive disorders. Cecropia pachystachya is a tropical tree species of Central and South America and a rich source of polyphenols, particularly flavonoids. The aim of this study was to characterize the flavonoid profile of an enriched flavonoid fraction of C. pachystachya (EFF-Cp) and evaluate the antidepressant-like effects of its acute administration in behavior, cytokine levels, oxidative stress and energy metabolism parameters. The EFF-Cp chemical characterization was performed by HPLC/DAD and LC/QTOF. The antidepressant-like effects were performed by the forced swimming test, splash test and open field test. EFF-Cp revealed 15 flavonoids, including seven new glycosyl flavonoids for C. pachystachya. Quantitatively, EFF-Cp showed isoorientin (43.46 mg/g), orientin (23.42 mg/g) and isovitexin (17.45 mg/g) as major C-glycosyl flavonoids. In addition, EFF-Cp at doses 50 and 100 mg/kg reduced the immobility time in the forced swimming test, without changing the locomotor activity and grooming time. In addition, EFF-Cp was able to prevent the oxidative damage in some brain areas. In conclusion, the results of this study suggest that EFF-Cp exerts antidepressant-like effects with its antioxidant properties.

Original languageEnglish (US)
Article numbere3982
JournalBiomedical Chromatography
Volume31
Issue number11
DOIs
StatePublished - Nov 2017
Externally publishedYes

Keywords

  • C-glycosyl flavonoids
  • Cecropia pachystachya
  • isoorientin
  • major depressive disorder
  • oxidative stress

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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