TY - JOUR
T1 - LC/QTOF profile and preliminary stability studies of an enriched flavonoid fraction of Cecropia pachystachya Trécul leaves with potential antidepressant-like activity
AU - Ortmann, Caroline Flach
AU - Abelaira, Helena Mendes
AU - Réus, Gislaine Zilli
AU - Ignácio, Zuleide Maria
AU - Chaves, Vitor Clasen
AU - dos Santos, Talitha Caldas
AU - de Carvalho, Pâmela
AU - Carlessi, Anelise Scussel
AU - Bruchchen, Livia
AU - Danielski, Lucineia G.
AU - Cardoso, Simone Gonçalves
AU - de Campos, Angela Machado
AU - Petronilho, Fabricia
AU - Rebelo, Joyce
AU - dos Santos Morais, Meline Oliveira
AU - Vuolo, Francieli
AU - Dal-Pizzol, Felipe
AU - Streck, Emilio Luiz
AU - Quevedo, João
AU - Reginatto, Flávio Henrique
N1 - Publisher Copyright:
Copyright © 2017 John Wiley & Sons, Ltd.
PY - 2017/11
Y1 - 2017/11
N2 - There is increasing interest in natural antioxidants that are candidates for the prevention of brain damage occurring in major depressive disorders. Cecropia pachystachya is a tropical tree species of Central and South America and a rich source of polyphenols, particularly flavonoids. The aim of this study was to characterize the flavonoid profile of an enriched flavonoid fraction of C. pachystachya (EFF-Cp) and evaluate the antidepressant-like effects of its acute administration in behavior, cytokine levels, oxidative stress and energy metabolism parameters. The EFF-Cp chemical characterization was performed by HPLC/DAD and LC/QTOF. The antidepressant-like effects were performed by the forced swimming test, splash test and open field test. EFF-Cp revealed 15 flavonoids, including seven new glycosyl flavonoids for C. pachystachya. Quantitatively, EFF-Cp showed isoorientin (43.46 mg/g), orientin (23.42 mg/g) and isovitexin (17.45 mg/g) as major C-glycosyl flavonoids. In addition, EFF-Cp at doses 50 and 100 mg/kg reduced the immobility time in the forced swimming test, without changing the locomotor activity and grooming time. In addition, EFF-Cp was able to prevent the oxidative damage in some brain areas. In conclusion, the results of this study suggest that EFF-Cp exerts antidepressant-like effects with its antioxidant properties.
AB - There is increasing interest in natural antioxidants that are candidates for the prevention of brain damage occurring in major depressive disorders. Cecropia pachystachya is a tropical tree species of Central and South America and a rich source of polyphenols, particularly flavonoids. The aim of this study was to characterize the flavonoid profile of an enriched flavonoid fraction of C. pachystachya (EFF-Cp) and evaluate the antidepressant-like effects of its acute administration in behavior, cytokine levels, oxidative stress and energy metabolism parameters. The EFF-Cp chemical characterization was performed by HPLC/DAD and LC/QTOF. The antidepressant-like effects were performed by the forced swimming test, splash test and open field test. EFF-Cp revealed 15 flavonoids, including seven new glycosyl flavonoids for C. pachystachya. Quantitatively, EFF-Cp showed isoorientin (43.46 mg/g), orientin (23.42 mg/g) and isovitexin (17.45 mg/g) as major C-glycosyl flavonoids. In addition, EFF-Cp at doses 50 and 100 mg/kg reduced the immobility time in the forced swimming test, without changing the locomotor activity and grooming time. In addition, EFF-Cp was able to prevent the oxidative damage in some brain areas. In conclusion, the results of this study suggest that EFF-Cp exerts antidepressant-like effects with its antioxidant properties.
KW - C-glycosyl flavonoids
KW - Cecropia pachystachya
KW - isoorientin
KW - major depressive disorder
KW - oxidative stress
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U2 - 10.1002/bmc.3982
DO - 10.1002/bmc.3982
M3 - Article
C2 - 28370241
AN - SCOPUS:85019994571
SN - 0269-3879
VL - 31
JO - Biomedical Chromatography
JF - Biomedical Chromatography
IS - 11
M1 - e3982
ER -