Leishmania donovani: Expression and characterization of Escherichia coli-expressed recombinant chitinase LdCHT1

Abdel Razek-Desouky, Charles A. Specht, Lynn Soong, Joseph M. Vinetz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Leishmania parasites produce chitinase activity (EC. 3.2.1.14) thought to be important in parasite-sandfly interactions and transmission of the parasite to the vertebrate host. Previous observations have suggested that parasite chitinases are involved in degradation of the sandfly peritrophic matrix and the chitinous layer of the cardiac valve cuticle. This chitinase activity is thought to produce an incompetent pharyngeal valve sphincter and a route of egress that allow Leishmania promastigotes to be regurgitated into the site of blood feeding. In the studies reported here, enzymatically active L. donovani chitinase LdCHT1 was expressed as a thioredoxin fusion protein in Escherichia coli strain AD494 (DE3). Recombinant LdCHT1 had a predominantly endochitinase activity, in contrast to previous reports of both exoand endochitinase activities in axenic culture supernatants of diverse Leishmania spp. promastigotes. The predominant endochitinase activity of recombinant LdCHT1 is consistent with the presumed function of the enzyme in disrupting chitinous structures in the sandfly digestive system to allow transmission.

Original languageEnglish (US)
Pages (from-to)220-225
Number of pages6
JournalExperimental Parasitology
Volume99
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Leishmania donovani
Chitinases
Escherichia coli
Psychodidae
Leishmania
Parasites
Axenic Culture
Digestive System
Thioredoxins
Escherichia coli Proteins
Heart Valves
Vertebrates
Enzymes

ASJC Scopus subject areas

  • Immunology
  • Parasitology
  • Infectious Diseases

Cite this

Leishmania donovani : Expression and characterization of Escherichia coli-expressed recombinant chitinase LdCHT1. / Razek-Desouky, Abdel; Specht, Charles A.; Soong, Lynn; Vinetz, Joseph M.

In: Experimental Parasitology, Vol. 99, No. 4, 2001, p. 220-225.

Research output: Contribution to journalArticle

Razek-Desouky, Abdel ; Specht, Charles A. ; Soong, Lynn ; Vinetz, Joseph M. / Leishmania donovani : Expression and characterization of Escherichia coli-expressed recombinant chitinase LdCHT1. In: Experimental Parasitology. 2001 ; Vol. 99, No. 4. pp. 220-225.
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