Leptin resistance of adipocytes in obesity: Role of suppressors of cytokine signaling

Zhuowei Wang, Yan Ting Zhou, Tetsuya Kakuma, Young Lee, Satya P. Kalra, Pushpa S. Kalra, Wentong Pan, Roger H. Unger

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Liver-derived hyperleptinemia induced in normal rats by adenovirus-induced gene transfer causes rapid disappearance of body fat, whereas the endogenous adipocyte-derived hyperleptinemia of obesity does not. Here we induce liver-derived hyperleptinemia in rats with adipocyte-derived hyperleptinemia of acquired obesity caused by ventromedial hypothalamus lesioning (VMH rats) or by feeding 60% fat (DIO rats). Liver-derived hyperleptinemia in obese rats caused only a 5-7% loss of body weight, compared to a 13% loss in normoleptinemic lean animals; but in actual grams of weight lost there was no significant difference between obese and lean groups, suggesting that a subset of cells remain leptin-sensitive in obesity. mRNA and protein of a putative leptin-resistance factor, suppressor of cytokine signaling (SOCS)-1 or -3, were both increased in white adipose tissues (WAT) of VMH and DIO rats. Since transgenic overexpression of SOCS-3 in islets reduced the lipopenic effect of leptin by 75%, we conclude that the increased expression of SOCS-1 and -3 in WAT of rats with acquired obesity could have blocked leptin's lipopenic action in the leptin-resistant WAT population. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)20-26
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume277
Issue number1
DOIs
StatePublished - Oct 14 2000
Externally publishedYes

Fingerprint

Leptin
Adipocytes
Rats
Obesity
Cytokines
White Adipose Tissue
Liver
Tissue
Fats
Gene transfer
R Factors
Adenoviridae
Hypothalamus
Adipose Tissue
Animals
Body Weight
Weights and Measures
Messenger RNA
Population
Genes

Keywords

  • Direct leptin action
  • Endocrine autosuppression
  • Leptin resistance
  • Obesity
  • SOCS

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Wang, Z., Zhou, Y. T., Kakuma, T., Lee, Y., Kalra, S. P., Kalra, P. S., ... Unger, R. H. (2000). Leptin resistance of adipocytes in obesity: Role of suppressors of cytokine signaling. Biochemical and Biophysical Research Communications, 277(1), 20-26. https://doi.org/10.1006/bbrc.2000.3615

Leptin resistance of adipocytes in obesity : Role of suppressors of cytokine signaling. / Wang, Zhuowei; Zhou, Yan Ting; Kakuma, Tetsuya; Lee, Young; Kalra, Satya P.; Kalra, Pushpa S.; Pan, Wentong; Unger, Roger H.

In: Biochemical and Biophysical Research Communications, Vol. 277, No. 1, 14.10.2000, p. 20-26.

Research output: Contribution to journalArticle

Wang, Zhuowei ; Zhou, Yan Ting ; Kakuma, Tetsuya ; Lee, Young ; Kalra, Satya P. ; Kalra, Pushpa S. ; Pan, Wentong ; Unger, Roger H. / Leptin resistance of adipocytes in obesity : Role of suppressors of cytokine signaling. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 277, No. 1. pp. 20-26.
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