Leucine kinetics at graded intakes in young men

Quantitative fate of dietary leucine

Joaquin Cortiella, D. E. Matthews, R. A. Hoerr, D. M. Bier, V. R. Young

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

To explore leucine metabolism in relation to leucine intake, five young adult men received an L-amino acid diet that supplied 40, 30, 20, and 10 mg leucine · kg-1 · d-1 for 6 d. A stable-isotope-tracer infusion study was then conducted for 5 h while subjects received an intragastric infusion of the test diet. Primed, constant infusions of L-[1-13C]leucine (intragastric) and L-[2H3]leucine (intravenous) were given simultaneously. A final infusion study was conducted in subjects in the postabsorptive state after an additional 2 d with the 10-mg diet. Estimates were made of leucine flux and oxidation, rates of uptake and release of absorbed leucine by the splanchnic region, and leucine balance. The rate of appearance of dietary leucine in the systemic circulation (Leu(d)) decreased (p < 0.01) between the 40- and 10-mg diets. At the latter intake, splanchnic uptake was ~ 37% of absorbed leucine. The correlation between Leu(d) and plasma leucine concentration was highly positive. A leucine intake of ~ 40 mg · kg-1 · d-1 was close to that required to maintain leucine balance under these conditions.

Original languageEnglish (US)
Pages (from-to)998-1009
Number of pages12
JournalAmerican Journal of Clinical Nutrition
Volume48
Issue number4
StatePublished - 1988
Externally publishedYes

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Leucine
leucine
kinetics
Diet
Viscera
diet
uptake mechanisms
isotope labeling
young adults
Isotopes
experimental diets
stable isotopes
Young Adult
oxidation
Amino Acids

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Leucine kinetics at graded intakes in young men : Quantitative fate of dietary leucine. / Cortiella, Joaquin; Matthews, D. E.; Hoerr, R. A.; Bier, D. M.; Young, V. R.

In: American Journal of Clinical Nutrition, Vol. 48, No. 4, 1988, p. 998-1009.

Research output: Contribution to journalArticle

Cortiella, Joaquin ; Matthews, D. E. ; Hoerr, R. A. ; Bier, D. M. ; Young, V. R. / Leucine kinetics at graded intakes in young men : Quantitative fate of dietary leucine. In: American Journal of Clinical Nutrition. 1988 ; Vol. 48, No. 4. pp. 998-1009.
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