Leucine partially protects muscle mass and function during bed rest in middle-aged adults

Kirk L. English, Joni A. Mettler, Jennifer B. Ellison, Madonna M. Mamerow, Emily Arentson-Lantz, James M. Pattarini, Robert Ploutz-Snyder, Melinda Sheffield-Moore, Douglas Paddon-Jones

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: Physical inactivity triggers a rapid loss of muscle mass and function in older adults. Middle-aged adults show few phenotypic signs of aging yet may be more susceptible to inactivity than younger adults. Objective: The aim was to determine whether leucine, a stimulator of translation initiation and skeletal muscle protein synthesis (MPS), can protect skeletal muscle health during bed rest. Design: We used a randomized, double-blind, placebo-controlled trial to assess changes in skeletal MPS, cellular signaling, body composition, and skeletal muscle function in middle-aged adults (n = 19; age ± SEM: 52 ± 1 y) in response to leucine supplementation (LEU group: 0.06 g kg-1 meal-1) or an alanine control (CON group) during 14 d of bed rest. Results: Bed rest decreased postabsorptive MPS by 30% ± 9% (CON group) and by 10% ± 10% (LEU group) (main effect for time, P <0.05), but no differences between groups with respect to pre-post changes (group time interactions) were detected for MPS or cell signaling. Leucine protected knee extensor peak torque (CON compared with LEU group: -15% ± 2% and -7% ± 3%; group time interaction, P <0.05) and endurance (CON compared with LEU: -14% ± 3% and -2% ± 4%; group time interaction, P <0.05), prevented an increase in body fat percentage (group 3 time interaction, P <0.05), and reduced whole-body lean mass loss after 7 d (CON compared with LEU: -1.5 ± 0.3 and -0.8 ± 0.3 kg; group time interaction, P <0.05) but not 14 d (CON compared with LEU: -1.5 ± 0.3 and -1.0 ± 0.3 kg) of bed rest. Leucine also maintained muscle quality (peak torque/kg leg lean mass) after 14 d of bed-rest inactivity (CON compared with LEU: 29% ± 2% and +1% ± 3%; group time interaction, P <0.05). Conclusions: Bed rest has a profoundly negative effect on muscle metabolism, mass, and function in middle-aged adults. Leucine supplementation may partially protect muscle health during relatively brief periods of physical inactivity.

Original languageEnglish (US)
Pages (from-to)465-473
Number of pages9
JournalAmerican Journal of Clinical Nutrition
Volume103
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Bed Rest
Leucine
Muscle Proteins
Muscles
Skeletal Muscle
Torque
Health
Body Composition
Alanine
Muscle Cells
Meals
Adipose Tissue
Young Adult
Leg
Knee
Placebos
Control Groups

Keywords

  • Atrophy
  • Dietary supplementation
  • Nutrition
  • Physical inactivity
  • Skeletal muscle protein synthesis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

English, K. L., Mettler, J. A., Ellison, J. B., Mamerow, M. M., Arentson-Lantz, E., Pattarini, J. M., ... Paddon-Jones, D. (2016). Leucine partially protects muscle mass and function during bed rest in middle-aged adults. American Journal of Clinical Nutrition, 103(2), 465-473. https://doi.org/10.3945/ajcn.115.112359

Leucine partially protects muscle mass and function during bed rest in middle-aged adults. / English, Kirk L.; Mettler, Joni A.; Ellison, Jennifer B.; Mamerow, Madonna M.; Arentson-Lantz, Emily; Pattarini, James M.; Ploutz-Snyder, Robert; Sheffield-Moore, Melinda; Paddon-Jones, Douglas.

In: American Journal of Clinical Nutrition, Vol. 103, No. 2, 01.02.2016, p. 465-473.

Research output: Contribution to journalArticle

English, KL, Mettler, JA, Ellison, JB, Mamerow, MM, Arentson-Lantz, E, Pattarini, JM, Ploutz-Snyder, R, Sheffield-Moore, M & Paddon-Jones, D 2016, 'Leucine partially protects muscle mass and function during bed rest in middle-aged adults', American Journal of Clinical Nutrition, vol. 103, no. 2, pp. 465-473. https://doi.org/10.3945/ajcn.115.112359
English KL, Mettler JA, Ellison JB, Mamerow MM, Arentson-Lantz E, Pattarini JM et al. Leucine partially protects muscle mass and function during bed rest in middle-aged adults. American Journal of Clinical Nutrition. 2016 Feb 1;103(2):465-473. https://doi.org/10.3945/ajcn.115.112359
English, Kirk L. ; Mettler, Joni A. ; Ellison, Jennifer B. ; Mamerow, Madonna M. ; Arentson-Lantz, Emily ; Pattarini, James M. ; Ploutz-Snyder, Robert ; Sheffield-Moore, Melinda ; Paddon-Jones, Douglas. / Leucine partially protects muscle mass and function during bed rest in middle-aged adults. In: American Journal of Clinical Nutrition. 2016 ; Vol. 103, No. 2. pp. 465-473.
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abstract = "Background: Physical inactivity triggers a rapid loss of muscle mass and function in older adults. Middle-aged adults show few phenotypic signs of aging yet may be more susceptible to inactivity than younger adults. Objective: The aim was to determine whether leucine, a stimulator of translation initiation and skeletal muscle protein synthesis (MPS), can protect skeletal muscle health during bed rest. Design: We used a randomized, double-blind, placebo-controlled trial to assess changes in skeletal MPS, cellular signaling, body composition, and skeletal muscle function in middle-aged adults (n = 19; age ± SEM: 52 ± 1 y) in response to leucine supplementation (LEU group: 0.06 g kg-1 meal-1) or an alanine control (CON group) during 14 d of bed rest. Results: Bed rest decreased postabsorptive MPS by 30{\%} ± 9{\%} (CON group) and by 10{\%} ± 10{\%} (LEU group) (main effect for time, P <0.05), but no differences between groups with respect to pre-post changes (group time interactions) were detected for MPS or cell signaling. Leucine protected knee extensor peak torque (CON compared with LEU group: -15{\%} ± 2{\%} and -7{\%} ± 3{\%}; group time interaction, P <0.05) and endurance (CON compared with LEU: -14{\%} ± 3{\%} and -2{\%} ± 4{\%}; group time interaction, P <0.05), prevented an increase in body fat percentage (group 3 time interaction, P <0.05), and reduced whole-body lean mass loss after 7 d (CON compared with LEU: -1.5 ± 0.3 and -0.8 ± 0.3 kg; group time interaction, P <0.05) but not 14 d (CON compared with LEU: -1.5 ± 0.3 and -1.0 ± 0.3 kg) of bed rest. Leucine also maintained muscle quality (peak torque/kg leg lean mass) after 14 d of bed-rest inactivity (CON compared with LEU: 29{\%} ± 2{\%} and +1{\%} ± 3{\%}; group time interaction, P <0.05). Conclusions: Bed rest has a profoundly negative effect on muscle metabolism, mass, and function in middle-aged adults. Leucine supplementation may partially protect muscle health during relatively brief periods of physical inactivity.",
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AU - Arentson-Lantz, Emily

AU - Pattarini, James M.

AU - Ploutz-Snyder, Robert

AU - Sheffield-Moore, Melinda

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N2 - Background: Physical inactivity triggers a rapid loss of muscle mass and function in older adults. Middle-aged adults show few phenotypic signs of aging yet may be more susceptible to inactivity than younger adults. Objective: The aim was to determine whether leucine, a stimulator of translation initiation and skeletal muscle protein synthesis (MPS), can protect skeletal muscle health during bed rest. Design: We used a randomized, double-blind, placebo-controlled trial to assess changes in skeletal MPS, cellular signaling, body composition, and skeletal muscle function in middle-aged adults (n = 19; age ± SEM: 52 ± 1 y) in response to leucine supplementation (LEU group: 0.06 g kg-1 meal-1) or an alanine control (CON group) during 14 d of bed rest. Results: Bed rest decreased postabsorptive MPS by 30% ± 9% (CON group) and by 10% ± 10% (LEU group) (main effect for time, P <0.05), but no differences between groups with respect to pre-post changes (group time interactions) were detected for MPS or cell signaling. Leucine protected knee extensor peak torque (CON compared with LEU group: -15% ± 2% and -7% ± 3%; group time interaction, P <0.05) and endurance (CON compared with LEU: -14% ± 3% and -2% ± 4%; group time interaction, P <0.05), prevented an increase in body fat percentage (group 3 time interaction, P <0.05), and reduced whole-body lean mass loss after 7 d (CON compared with LEU: -1.5 ± 0.3 and -0.8 ± 0.3 kg; group time interaction, P <0.05) but not 14 d (CON compared with LEU: -1.5 ± 0.3 and -1.0 ± 0.3 kg) of bed rest. Leucine also maintained muscle quality (peak torque/kg leg lean mass) after 14 d of bed-rest inactivity (CON compared with LEU: 29% ± 2% and +1% ± 3%; group time interaction, P <0.05). Conclusions: Bed rest has a profoundly negative effect on muscle metabolism, mass, and function in middle-aged adults. Leucine supplementation may partially protect muscle health during relatively brief periods of physical inactivity.

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KW - Atrophy

KW - Dietary supplementation

KW - Nutrition

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KW - Skeletal muscle protein synthesis

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