Levels of mitochondrial reactive oxygen species increase in rat neuropathic spinal dorsal horn neurons

Eun Sung Park; Xiu Gao; Jin Mo Chung; Kyungsoon Chung

doi:10.1016/j.neulet.2005.08.055

Levels of mitochondrial reactive oxygen species increase in rat neuropathic spinal dorsal horn neurons

Eun Sung Park, Xiu Gao, Jin Mo Chung, Kyungsoon Chung

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128 Scopus citations

Abstract

Reactive oxygen species (ROS) are toxic agents that may be involved in various neurodegenerative diseases. Recent studies indicate that ROS are also involved in persistent pain through a spinal mechanism. Since the major source of ROS in neurons is mitochondria, mitochondrial ROS generation was examined in dorsal horn neurons of neuropathic rats. Neuropathic rats were produced by L5 spinal nerve ligation and mitochondrial ROS was detected by the mitochondrial marker, Mitotracker Red^® CM-H₂XRos (MT-Red). Neurons were identified immunohistochemically for the neuronal marker NeuN. The number of MT-Red positive cells was increased 60-100% in the neuropathic dorsal horn. Approximately 75-85% of MT-Red positive cells were neurons. These data suggest that increased mitochondrial ROS in dorsal horn neurons may contribute to central sensitization in neuropathic rats.

Original language	English (US)
Pages (from-to)	108-111
Number of pages	4
Journal	Neuroscience Letters
Volume	391
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2005.08.055
State	Published - Jan 2 2006
Externally published	Yes

Keywords

Central sensitisation
Free radicals
Mitotracker
Persistent pain
ROS

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2005.08.055

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title = "Levels of mitochondrial reactive oxygen species increase in rat neuropathic spinal dorsal horn neurons",

abstract = "Reactive oxygen species (ROS) are toxic agents that may be involved in various neurodegenerative diseases. Recent studies indicate that ROS are also involved in persistent pain through a spinal mechanism. Since the major source of ROS in neurons is mitochondria, mitochondrial ROS generation was examined in dorsal horn neurons of neuropathic rats. Neuropathic rats were produced by L5 spinal nerve ligation and mitochondrial ROS was detected by the mitochondrial marker, Mitotracker Red{\textregistered} CM-H2XRos (MT-Red). Neurons were identified immunohistochemically for the neuronal marker NeuN. The number of MT-Red positive cells was increased 60-100% in the neuropathic dorsal horn. Approximately 75-85% of MT-Red positive cells were neurons. These data suggest that increased mitochondrial ROS in dorsal horn neurons may contribute to central sensitization in neuropathic rats.",

keywords = "Central sensitisation, Free radicals, Mitotracker, Persistent pain, ROS",

author = "Park, {Eun Sung} and Xiu Gao and Chung, {Jin Mo} and Kyungsoon Chung",

note = "Funding Information: This work was supported by National Institutes of Health Grants NS11255 and NS31680. We would like to express our gratitude to Ms. Denise Broker for her excellent assistance in editing the manuscript.",

year = "2006",

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doi = "10.1016/j.neulet.2005.08.055",

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AU - Park, Eun Sung

AU - Gao, Xiu

AU - Chung, Jin Mo

AU - Chung, Kyungsoon

N1 - Funding Information: This work was supported by National Institutes of Health Grants NS11255 and NS31680. We would like to express our gratitude to Ms. Denise Broker for her excellent assistance in editing the manuscript.

PY - 2006/1/2

Y1 - 2006/1/2

N2 - Reactive oxygen species (ROS) are toxic agents that may be involved in various neurodegenerative diseases. Recent studies indicate that ROS are also involved in persistent pain through a spinal mechanism. Since the major source of ROS in neurons is mitochondria, mitochondrial ROS generation was examined in dorsal horn neurons of neuropathic rats. Neuropathic rats were produced by L5 spinal nerve ligation and mitochondrial ROS was detected by the mitochondrial marker, Mitotracker Red® CM-H2XRos (MT-Red). Neurons were identified immunohistochemically for the neuronal marker NeuN. The number of MT-Red positive cells was increased 60-100% in the neuropathic dorsal horn. Approximately 75-85% of MT-Red positive cells were neurons. These data suggest that increased mitochondrial ROS in dorsal horn neurons may contribute to central sensitization in neuropathic rats.

AB - Reactive oxygen species (ROS) are toxic agents that may be involved in various neurodegenerative diseases. Recent studies indicate that ROS are also involved in persistent pain through a spinal mechanism. Since the major source of ROS in neurons is mitochondria, mitochondrial ROS generation was examined in dorsal horn neurons of neuropathic rats. Neuropathic rats were produced by L5 spinal nerve ligation and mitochondrial ROS was detected by the mitochondrial marker, Mitotracker Red® CM-H2XRos (MT-Red). Neurons were identified immunohistochemically for the neuronal marker NeuN. The number of MT-Red positive cells was increased 60-100% in the neuropathic dorsal horn. Approximately 75-85% of MT-Red positive cells were neurons. These data suggest that increased mitochondrial ROS in dorsal horn neurons may contribute to central sensitization in neuropathic rats.

KW - Central sensitisation

KW - Free radicals

KW - Mitotracker

KW - Persistent pain

KW - ROS

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ER -

Levels of mitochondrial reactive oxygen species increase in rat neuropathic spinal dorsal horn neurons

Abstract

Keywords

ASJC Scopus subject areas

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