Liao ning virus, a new Chinese seadornavirus that replicates in transformed and embryonic mammalian cells

Houssam Attoui, Fauziah Mohd Jaafar, Mourad Belhouchet, Sanju Tao, Boquan Chen, Guodong Liang, Robert B. Tesh, Philippe de Micco, Xavier de Lamballerie

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Abstract

Seadornaviruses are emerging arboviral pathogens from the south-east of Asia. The genus Seadornavirus contains two distinct species, Banna virus (BAV) isolated from humans with encephalitis and Kadipiro virus. BAV replicates within insect cells and mice but not in cultured mammalian cells. Here, the discovery of Liao ning virus (LNV), a new seadornavirus from the Aedes dorsalis mosquito, which was completely sequenced and was found to be related to BAV and Kadipiro virus, is reported. Two serotypes of LNV could be distinguished by a serum neutralization assay. According to amino acid identity with other seadornaviruses, and to criteria set by the ICTV for species delineation, LNV was identified as a member of a new species of virus. Its morphology was characterized by electron microscopy and found to be similar to that of BAV. LNV is the first reported seadornavirus that replicates in mammalian cells, leading to massive cytopathic effect in all transformed or embryonic cell lines tested. LNV- and BAV-infected mice producing a viraemia lasting for 5 days was followed by viral clearance. Mice infection generated virus quasi-species for LNV (the first reported observation for quasi-species in the family Reoviridae) but not for BAV. Challenge with BAV in mice immunized against BAV did not lead to productive infection. However, challenge with LNV in mice immunized against LNV was lethal with a new phase of viraemia and massive haemorrhage.

Original languageEnglish (US)
Pages (from-to)199-208
Number of pages10
JournalJournal of General Virology
Volume87
Issue number1
DOIs
StatePublished - Jan 2006

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Coltivirus
Reoviridae
Viruses
Viremia
Encephalitis Viruses
Far East
Aedes
Virus Diseases
Culicidae

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Attoui, H., Jaafar, F. M., Belhouchet, M., Tao, S., Chen, B., Liang, G., ... de Lamballerie, X. (2006). Liao ning virus, a new Chinese seadornavirus that replicates in transformed and embryonic mammalian cells. Journal of General Virology, 87(1), 199-208. https://doi.org/10.1099/vir.0.81294-0

Liao ning virus, a new Chinese seadornavirus that replicates in transformed and embryonic mammalian cells. / Attoui, Houssam; Jaafar, Fauziah Mohd; Belhouchet, Mourad; Tao, Sanju; Chen, Boquan; Liang, Guodong; Tesh, Robert B.; de Micco, Philippe; de Lamballerie, Xavier.

In: Journal of General Virology, Vol. 87, No. 1, 01.2006, p. 199-208.

Research output: Contribution to journalArticle

Attoui, H, Jaafar, FM, Belhouchet, M, Tao, S, Chen, B, Liang, G, Tesh, RB, de Micco, P & de Lamballerie, X 2006, 'Liao ning virus, a new Chinese seadornavirus that replicates in transformed and embryonic mammalian cells', Journal of General Virology, vol. 87, no. 1, pp. 199-208. https://doi.org/10.1099/vir.0.81294-0
Attoui, Houssam ; Jaafar, Fauziah Mohd ; Belhouchet, Mourad ; Tao, Sanju ; Chen, Boquan ; Liang, Guodong ; Tesh, Robert B. ; de Micco, Philippe ; de Lamballerie, Xavier. / Liao ning virus, a new Chinese seadornavirus that replicates in transformed and embryonic mammalian cells. In: Journal of General Virology. 2006 ; Vol. 87, No. 1. pp. 199-208.
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