Light‐scattering spectroscopic study of polysaccharide protein conjugate

A. Patkowski, W. Bujalowski, B. Chu, R. Schneerson, J. B. Robbins

Research output: Contribution to journalArticlepeer-review


By combining gel permeation chromatography (GPC) and light‐scattering spectroscopy, including photon correlation and angular distribution of absolute scattered intensity, we were able to characterize immunologically active Haemophilus influenzae type b polysaccharide (HIB Ps) bovine serum albumin (BSA) conjugates in terms of equivalent hydrodynamic radius rh ∼ (6.2 ± 0.6) × 102 Å, apparent radius of gyration rg ∼ (5.4 ± 0.3) × 102 Å, apparent molecular weight Mw ∼ (3.5 ± 0.4) × 106 g/mol, and a second virial coefficient A2 ∼ (1.9 ± 0.3) × 10−4 cm3 mol/g2. We could study the effects of each of the processes in the conjugate formation according to the following procedure: BSA (dialysis, modification, fractionation) + HIB Ps → HIB Ps/BSA conjugate (conjugate formation, fractionation). Narrow distributions of HIB Ps BSA conjugate formation can be achieved using fractionated BSA.

Original languageEnglish (US)
Pages (from-to)1503-1520
Number of pages18
Issue number8
StatePublished - Aug 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry


Dive into the research topics of 'Light‐scattering spectroscopic study of polysaccharide protein conjugate'. Together they form a unique fingerprint.

Cite this