Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon

Maya Williams, Sandra V. Mayer, William L. Johnson, Rubing Chen, Evgeniya Volkova, Stalin Vilcarromero, Steven G. Widen, Thomas G. Wood, Luis Suarez-Ognio, Kanya C. Long, Kathryn A. Hanley, Amy C. Morrison, Nikos Vasilakis, Eric S. Halsey

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25 Scopus citations

Abstract

During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak. Copyright

Original languageEnglish (US)
Pages (from-to)611-620
Number of pages10
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume91
Issue number3
DOIs
StatePublished - Sep 1 2014

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ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Williams, M., Mayer, S. V., Johnson, W. L., Chen, R., Volkova, E., Vilcarromero, S., Widen, S. G., Wood, T. G., Suarez-Ognio, L., Long, K. C., Hanley, K. A., Morrison, A. C., Vasilakis, N., & Halsey, E. S. (2014). Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon. American Journal of Tropical Medicine and Hygiene, 91(3), 611-620. https://doi.org/10.4269/ajtmh.13-0600