Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon

Maya Williams, Sandra V. Mayer, William L. Johnson, Rubing Chen, Evgeniya Volkova, Stalin Vilcarromero, Steven Widen, Thomas Wood, Luis Suarez-Ognio, Kanya C. Long, Kathryn A. Hanley, Amy C. Morrison, Nikos Vasilakis, Eric S. Halsey

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak. Copyright

Original languageEnglish (US)
Pages (from-to)611-620
Number of pages10
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume91
Issue number3
DOIs
StatePublished - Sep 1 2014

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Severe Dengue
Dengue Virus
Asian Americans
Disease Outbreaks
Peru
Dengue
South America
Aedes
Amino Acid Substitution
Dendritic Cells
Brazil
Monocytes
Genotype
Genome
Viruses

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology
  • Medicine(all)

Cite this

Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon. / Williams, Maya; Mayer, Sandra V.; Johnson, William L.; Chen, Rubing; Volkova, Evgeniya; Vilcarromero, Stalin; Widen, Steven; Wood, Thomas; Suarez-Ognio, Luis; Long, Kanya C.; Hanley, Kathryn A.; Morrison, Amy C.; Vasilakis, Nikos; Halsey, Eric S.

In: American Journal of Tropical Medicine and Hygiene, Vol. 91, No. 3, 01.09.2014, p. 611-620.

Research output: Contribution to journalArticle

Williams, M, Mayer, SV, Johnson, WL, Chen, R, Volkova, E, Vilcarromero, S, Widen, S, Wood, T, Suarez-Ognio, L, Long, KC, Hanley, KA, Morrison, AC, Vasilakis, N & Halsey, ES 2014, 'Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon', American Journal of Tropical Medicine and Hygiene, vol. 91, no. 3, pp. 611-620. https://doi.org/10.4269/ajtmh.13-0600
Williams, Maya ; Mayer, Sandra V. ; Johnson, William L. ; Chen, Rubing ; Volkova, Evgeniya ; Vilcarromero, Stalin ; Widen, Steven ; Wood, Thomas ; Suarez-Ognio, Luis ; Long, Kanya C. ; Hanley, Kathryn A. ; Morrison, Amy C. ; Vasilakis, Nikos ; Halsey, Eric S. / Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the Peruvian Amazon. In: American Journal of Tropical Medicine and Hygiene. 2014 ; Vol. 91, No. 3. pp. 611-620.
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