Linkage disequilibrium between HLA-DPB1 alleles and retinoid X receptor β haplotypes

Ricardo Rajsbaum Gorodezky, Dolores Fici, Deborah A. Boggs, Patricia A. Fraser, Pedro O. Flores-Villanueva, Zuheir L. Awdeh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The human retinoid X receptor β (RXRB) gene maps to the major histocompatibility complex (MHC) region, between KE4 and COL11A2, approximately 130-kb centromeric to HLA-DPB1. We have recently reported a new polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to detect the G to T single nucleotide polymorphism (SNP) located seven nucleotides after the tenth exon of the RXRB gene, or 3′end+7 position according to existing nomenclature. We also reported strong linkage disequilibrium between the HLA-DPB1*0401 and RXRB+7*T alleles. In the present study, we describe two PCR-RFLP methods to detect additional SNPs in the RXRB gene, T to A, at exon10+378 and A to T at 3′end+140. This new methodology permitted the unambiguous assignment of three distinct SNPs at RXRB exon10+378, 3′end+7 and 3′end+140 to form an "RXRB haplotype." The data generated from this study were used to determine linkage disequilibrium between several MHC markers and the RXRB alleles and haplotypes. Family studies revealed significant linkage disequilibrium between the RXRB alleles and a number of HLA-DPB1 alleles.

Original languageEnglish (US)
Pages (from-to)771-778
Number of pages8
JournalHuman Immunology
Volume63
Issue number9
DOIs
StatePublished - Sep 2002
Externally publishedYes

Fingerprint

Retinoid X Receptors
Linkage Disequilibrium
Haplotypes
Alleles
Single Nucleotide Polymorphism
Major Histocompatibility Complex
Restriction Fragment Length Polymorphisms
Genes
Polymerase Chain Reaction
Terminology
Exons
Nucleotides
HLA-DPB1 antigen

Keywords

  • HLA-DPB1
  • Linkage disequilibrium
  • Polymorphism
  • Retinoid X receptor
  • SNP

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Linkage disequilibrium between HLA-DPB1 alleles and retinoid X receptor β haplotypes. / Rajsbaum Gorodezky, Ricardo; Fici, Dolores; Boggs, Deborah A.; Fraser, Patricia A.; Flores-Villanueva, Pedro O.; Awdeh, Zuheir L.

In: Human Immunology, Vol. 63, No. 9, 09.2002, p. 771-778.

Research output: Contribution to journalArticle

Rajsbaum Gorodezky, Ricardo ; Fici, Dolores ; Boggs, Deborah A. ; Fraser, Patricia A. ; Flores-Villanueva, Pedro O. ; Awdeh, Zuheir L. / Linkage disequilibrium between HLA-DPB1 alleles and retinoid X receptor β haplotypes. In: Human Immunology. 2002 ; Vol. 63, No. 9. pp. 771-778.
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