Lipid raft microdomains: A gateway for compartmentalized trafficking of Ebola and Marburg viruses

Sina Bavari, Catharine M. Bosio, Elizabeth Wiegand, Gordon Ruthel, Amy B. Will, Thomas W. Geisbert, Michael Hevey, Connie Schmaljohn, Alan Schmaljohn, M. Javad Aman

Research output: Contribution to journalArticlepeer-review

400 Scopus citations


Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses.

Original languageEnglish (US)
Pages (from-to)593-602
Number of pages10
JournalJournal of Experimental Medicine
Issue number5
StatePublished - Mar 4 2002
Externally publishedYes


  • Budding
  • Ebola
  • Filovirus
  • Rafts
  • VLP

ASJC Scopus subject areas

  • Immunology


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