Lipid raft microdomains

A gateway for compartmentalized trafficking of Ebola and Marburg viruses

Sina Bavari, Catharine M. Bosio, Elizabeth Wiegand, Gordon Ruthel, Amy B. Will, Thomas W. Geisbert, Michael Hevey, Connie Schmaljohn, Alan Schmaljohn, M. Javad Aman

Research output: Contribution to journalArticle

352 Citations (Scopus)

Abstract

Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses.

Original languageEnglish
Pages (from-to)593-602
Number of pages10
JournalJournal of Experimental Medicine
Volume195
Issue number5
DOIs
StatePublished - Mar 4 2002
Externally publishedYes

Fingerprint

Marburgvirus
Ebolavirus
Virion
Viruses
Lipids
Virus Assembly
Viral Proteins
Electron Microscopy
Glycoproteins
Vaccination
Infection
Proteins
Therapeutics

Keywords

  • Budding
  • Ebola
  • Filovirus
  • Rafts
  • VLP

ASJC Scopus subject areas

  • Immunology

Cite this

Lipid raft microdomains : A gateway for compartmentalized trafficking of Ebola and Marburg viruses. / Bavari, Sina; Bosio, Catharine M.; Wiegand, Elizabeth; Ruthel, Gordon; Will, Amy B.; Geisbert, Thomas W.; Hevey, Michael; Schmaljohn, Connie; Schmaljohn, Alan; Javad Aman, M.

In: Journal of Experimental Medicine, Vol. 195, No. 5, 04.03.2002, p. 593-602.

Research output: Contribution to journalArticle

Bavari, S, Bosio, CM, Wiegand, E, Ruthel, G, Will, AB, Geisbert, TW, Hevey, M, Schmaljohn, C, Schmaljohn, A & Javad Aman, M 2002, 'Lipid raft microdomains: A gateway for compartmentalized trafficking of Ebola and Marburg viruses', Journal of Experimental Medicine, vol. 195, no. 5, pp. 593-602. https://doi.org/10.1084/jem.20011500
Bavari, Sina ; Bosio, Catharine M. ; Wiegand, Elizabeth ; Ruthel, Gordon ; Will, Amy B. ; Geisbert, Thomas W. ; Hevey, Michael ; Schmaljohn, Connie ; Schmaljohn, Alan ; Javad Aman, M. / Lipid raft microdomains : A gateway for compartmentalized trafficking of Ebola and Marburg viruses. In: Journal of Experimental Medicine. 2002 ; Vol. 195, No. 5. pp. 593-602.
@article{189069d40b544b31bac363861214d92d,
title = "Lipid raft microdomains: A gateway for compartmentalized trafficking of Ebola and Marburg viruses",
abstract = "Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses.",
keywords = "Budding, Ebola, Filovirus, Rafts, VLP",
author = "Sina Bavari and Bosio, {Catharine M.} and Elizabeth Wiegand and Gordon Ruthel and Will, {Amy B.} and Geisbert, {Thomas W.} and Michael Hevey and Connie Schmaljohn and Alan Schmaljohn and {Javad Aman}, M.",
year = "2002",
month = "3",
day = "4",
doi = "10.1084/jem.20011500",
language = "English",
volume = "195",
pages = "593--602",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - Lipid raft microdomains

T2 - A gateway for compartmentalized trafficking of Ebola and Marburg viruses

AU - Bavari, Sina

AU - Bosio, Catharine M.

AU - Wiegand, Elizabeth

AU - Ruthel, Gordon

AU - Will, Amy B.

AU - Geisbert, Thomas W.

AU - Hevey, Michael

AU - Schmaljohn, Connie

AU - Schmaljohn, Alan

AU - Javad Aman, M.

PY - 2002/3/4

Y1 - 2002/3/4

N2 - Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses.

AB - Spatiotemporal aspects of filovirus entry and release are poorly understood. Lipid rafts act as functional platforms for multiple cellular signaling and trafficking processes. Here, we report the compartmentalization of Ebola and Marburg viral proteins within lipid rafts during viral assembly and budding. Filoviruses released from infected cells incorporated raft-associated molecules, suggesting that viral exit occurs at the rafts. Ectopic expression of Ebola matrix protein and glycoprotein supported raft-dependent release of filamentous, virus-like particles (VLPs), strikingly similar to live virus as revealed by electron microscopy. Our findings also revealed that the entry of filoviruses requires functional rafts, identifying rafts as the site of virus attack. The identification of rafts as the gateway for the entry and exit of filoviruses and raft-dependent generation of VLPs have important implications for development of therapeutics and vaccination strategies against infections with Ebola and Marburg viruses.

KW - Budding

KW - Ebola

KW - Filovirus

KW - Rafts

KW - VLP

UR - http://www.scopus.com/inward/record.url?scp=0037018099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037018099&partnerID=8YFLogxK

U2 - 10.1084/jem.20011500

DO - 10.1084/jem.20011500

M3 - Article

VL - 195

SP - 593

EP - 602

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 5

ER -