Lipoprotein distribution in sheep lung lymph

C. E. Cross, T. M. Forte, R. A. Gunther, George Kramer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The composition of lymph draining the sheep caudal mediastinal lymph node is believed to reflect the composition of lung interstitial fluid. Although long-term lymph fistulas have been extensively utilized in studies related to lung microvascular fluid and protein flux, attention has thus far not been focused on lipoproteins as they might appear in lung interstitial space. We therefore characterized the lipoprotein distributions and measured triglyceride and cholesterol concentrations of lung lymph and plasma in nine unanesthetized sheep with long-term embedded instrumentation. We also measured triglyceride and cholesterol concentrations in prefemoral and thoracic duct lymph. The plasma LDL concentration was 55.3 ± 20.2 mg/dl and HDL concentration was 99.9 ± 27.2 mg/dl. By comparison, the lung lymph LDL concentration was 27.7 ± 3.0 mg/dl and the HDL concentration was 45.4 ± 11 mg/dl. The major sheep lipoprotein classes (LDL, HDL), although low in concentration compared to humans, are present in lung lymph in relative proportions similar to those of plasma. Analytical ultracentrifugation revealed the presence of a fast-floating component in lung lymph HDL that was not present in plasma. Electron microscopy showed two sizes of particles in lung lymph HDL, 135Å and 97Å, which probably correspond to the fast-floating and slow-floating components, respectively; plasma HDL contained only 94 Å particles. changes in the physical properties of HDL present in lung lymph suggest modification of the HDL either in the endothelial barrier or interstitial fluid of the lung.

Original languageEnglish (US)
Pages (from-to)641-650
Number of pages10
JournalJournal of Laboratory and Clinical Medicine
Volume101
Issue number4
StatePublished - 1983
Externally publishedYes

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Lymph
Lipoproteins
Sheep
Plasmas
Lung
Fluids
Triglycerides
Cholesterol
Extracellular Fluid
Chemical analysis
Ducts
Electron microscopy
Physical properties
Fluxes
Thoracic Duct
Ultracentrifugation
LDL Lipoproteins
Particle Size
Fistula
oxidized low density lipoprotein

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Cross, C. E., Forte, T. M., Gunther, R. A., & Kramer, G. (1983). Lipoprotein distribution in sheep lung lymph. Journal of Laboratory and Clinical Medicine, 101(4), 641-650.

Lipoprotein distribution in sheep lung lymph. / Cross, C. E.; Forte, T. M.; Gunther, R. A.; Kramer, George.

In: Journal of Laboratory and Clinical Medicine, Vol. 101, No. 4, 1983, p. 641-650.

Research output: Contribution to journalArticle

Cross, CE, Forte, TM, Gunther, RA & Kramer, G 1983, 'Lipoprotein distribution in sheep lung lymph', Journal of Laboratory and Clinical Medicine, vol. 101, no. 4, pp. 641-650.
Cross, C. E. ; Forte, T. M. ; Gunther, R. A. ; Kramer, George. / Lipoprotein distribution in sheep lung lymph. In: Journal of Laboratory and Clinical Medicine. 1983 ; Vol. 101, No. 4. pp. 641-650.
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