Live, attenuated Venezuelan equine encephalitis virus vaccine (TC83) causes persistent brain infection in mice with non-functional αβ T-cells

Katherine Taylor, Olga Kolokoltsova, Shannon E. Ronca, Mark Estes, Slobodan Paessler

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Intranasal infection with vaccine strain of Venezuelan equine encephalitis virus (TC83) caused persistent viral infection in the brains of mice without functional αβ T-cells (αβ-TCR -/-). Remarkably, viral kinetics, host response gene transcripts and symptomatic disease are similar between αβ-TCR -/- and wild-type C57BL/6 (WT) mice during acute phase of infection [0-13 days post-infection (dpi)]. While WT mice clear infectious virus in the brain by 13 dpi, αβ-TCR -/- maintain infectious virus in the brain to 92 dpi. Persistent brain infection in αβ-TCR -/- correlated with inflammatory infiltrates and elevated cytokine protein levels in the brain at later time points. Persistent brain infection of αβ-TCR -/- mice provides a novel model to study prolonged alphaviral infection as well as the effects and biomarkers of long-term viral inflammation in the brain.

Original languageEnglish (US)
Article number81
JournalFrontiers in Microbiology
Volume8
Issue numberJAN
DOIs
StatePublished - Jan 26 2017

Fingerprint

Venezuelan Equine Encephalitis Viruses
Vaccines
T-Lymphocytes
Brain
Infection
Inbred C57BL Mouse
Viruses
Virus Diseases
Encephalitis
Biomarkers
Cytokines

Keywords

  • Alphavirus
  • Immunology
  • Persistent infection
  • TC83
  • VEEV

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Live, attenuated Venezuelan equine encephalitis virus vaccine (TC83) causes persistent brain infection in mice with non-functional αβ T-cells. / Taylor, Katherine; Kolokoltsova, Olga; Ronca, Shannon E.; Estes, Mark; Paessler, Slobodan.

In: Frontiers in Microbiology, Vol. 8, No. JAN, 81, 26.01.2017.

Research output: Contribution to journalArticle

@article{cd8a8391b9674a62ad6706aaa738e472,
title = "Live, attenuated Venezuelan equine encephalitis virus vaccine (TC83) causes persistent brain infection in mice with non-functional αβ T-cells",
abstract = "Intranasal infection with vaccine strain of Venezuelan equine encephalitis virus (TC83) caused persistent viral infection in the brains of mice without functional αβ T-cells (αβ-TCR -/-). Remarkably, viral kinetics, host response gene transcripts and symptomatic disease are similar between αβ-TCR -/- and wild-type C57BL/6 (WT) mice during acute phase of infection [0-13 days post-infection (dpi)]. While WT mice clear infectious virus in the brain by 13 dpi, αβ-TCR -/- maintain infectious virus in the brain to 92 dpi. Persistent brain infection in αβ-TCR -/- correlated with inflammatory infiltrates and elevated cytokine protein levels in the brain at later time points. Persistent brain infection of αβ-TCR -/- mice provides a novel model to study prolonged alphaviral infection as well as the effects and biomarkers of long-term viral inflammation in the brain.",
keywords = "Alphavirus, Immunology, Persistent infection, TC83, VEEV",
author = "Katherine Taylor and Olga Kolokoltsova and Ronca, {Shannon E.} and Mark Estes and Slobodan Paessler",
year = "2017",
month = "1",
day = "26",
doi = "10.3389/fmicb.2017.00081",
language = "English (US)",
volume = "8",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",
number = "JAN",

}

TY - JOUR

T1 - Live, attenuated Venezuelan equine encephalitis virus vaccine (TC83) causes persistent brain infection in mice with non-functional αβ T-cells

AU - Taylor, Katherine

AU - Kolokoltsova, Olga

AU - Ronca, Shannon E.

AU - Estes, Mark

AU - Paessler, Slobodan

PY - 2017/1/26

Y1 - 2017/1/26

N2 - Intranasal infection with vaccine strain of Venezuelan equine encephalitis virus (TC83) caused persistent viral infection in the brains of mice without functional αβ T-cells (αβ-TCR -/-). Remarkably, viral kinetics, host response gene transcripts and symptomatic disease are similar between αβ-TCR -/- and wild-type C57BL/6 (WT) mice during acute phase of infection [0-13 days post-infection (dpi)]. While WT mice clear infectious virus in the brain by 13 dpi, αβ-TCR -/- maintain infectious virus in the brain to 92 dpi. Persistent brain infection in αβ-TCR -/- correlated with inflammatory infiltrates and elevated cytokine protein levels in the brain at later time points. Persistent brain infection of αβ-TCR -/- mice provides a novel model to study prolonged alphaviral infection as well as the effects and biomarkers of long-term viral inflammation in the brain.

AB - Intranasal infection with vaccine strain of Venezuelan equine encephalitis virus (TC83) caused persistent viral infection in the brains of mice without functional αβ T-cells (αβ-TCR -/-). Remarkably, viral kinetics, host response gene transcripts and symptomatic disease are similar between αβ-TCR -/- and wild-type C57BL/6 (WT) mice during acute phase of infection [0-13 days post-infection (dpi)]. While WT mice clear infectious virus in the brain by 13 dpi, αβ-TCR -/- maintain infectious virus in the brain to 92 dpi. Persistent brain infection in αβ-TCR -/- correlated with inflammatory infiltrates and elevated cytokine protein levels in the brain at later time points. Persistent brain infection of αβ-TCR -/- mice provides a novel model to study prolonged alphaviral infection as well as the effects and biomarkers of long-term viral inflammation in the brain.

KW - Alphavirus

KW - Immunology

KW - Persistent infection

KW - TC83

KW - VEEV

UR - http://www.scopus.com/inward/record.url?scp=85011949160&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85011949160&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2017.00081

DO - 10.3389/fmicb.2017.00081

M3 - Article

AN - SCOPUS:85011949160

VL - 8

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - JAN

M1 - 81

ER -