Abstract
Intranasal infection with vaccine strain of Venezuelan equine encephalitis virus (TC83) caused persistent viral infection in the brains of mice without functional αβ T-cells (αβ-TCR -/-). Remarkably, viral kinetics, host response gene transcripts and symptomatic disease are similar between αβ-TCR -/- and wild-type C57BL/6 (WT) mice during acute phase of infection [0-13 days post-infection (dpi)]. While WT mice clear infectious virus in the brain by 13 dpi, αβ-TCR -/- maintain infectious virus in the brain to 92 dpi. Persistent brain infection in αβ-TCR -/- correlated with inflammatory infiltrates and elevated cytokine protein levels in the brain at later time points. Persistent brain infection of αβ-TCR -/- mice provides a novel model to study prolonged alphaviral infection as well as the effects and biomarkers of long-term viral inflammation in the brain.
| Original language | English (US) |
|---|---|
| Article number | 81 |
| Journal | Frontiers in Microbiology |
| Volume | 8 |
| Issue number | JAN |
| DOIs | |
| State | Published - Jan 26 2017 |
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Keywords
- Alphavirus
- Immunology
- Persistent infection
- TC83
- VEEV
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
Cite this
Live, attenuated Venezuelan equine encephalitis virus vaccine (TC83) causes persistent brain infection in mice with non-functional αβ T-cells. / Taylor, Katherine; Kolokoltsova, Olga; Ronca, Shannon E.; Estes, Mark; Paessler, Slobodan.
In: Frontiers in Microbiology, Vol. 8, No. JAN, 81, 26.01.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Live, attenuated Venezuelan equine encephalitis virus vaccine (TC83) causes persistent brain infection in mice with non-functional αβ T-cells
AU - Taylor, Katherine
AU - Kolokoltsova, Olga
AU - Ronca, Shannon E.
AU - Estes, Mark
AU - Paessler, Slobodan
PY - 2017/1/26
Y1 - 2017/1/26
N2 - Intranasal infection with vaccine strain of Venezuelan equine encephalitis virus (TC83) caused persistent viral infection in the brains of mice without functional αβ T-cells (αβ-TCR -/-). Remarkably, viral kinetics, host response gene transcripts and symptomatic disease are similar between αβ-TCR -/- and wild-type C57BL/6 (WT) mice during acute phase of infection [0-13 days post-infection (dpi)]. While WT mice clear infectious virus in the brain by 13 dpi, αβ-TCR -/- maintain infectious virus in the brain to 92 dpi. Persistent brain infection in αβ-TCR -/- correlated with inflammatory infiltrates and elevated cytokine protein levels in the brain at later time points. Persistent brain infection of αβ-TCR -/- mice provides a novel model to study prolonged alphaviral infection as well as the effects and biomarkers of long-term viral inflammation in the brain.
AB - Intranasal infection with vaccine strain of Venezuelan equine encephalitis virus (TC83) caused persistent viral infection in the brains of mice without functional αβ T-cells (αβ-TCR -/-). Remarkably, viral kinetics, host response gene transcripts and symptomatic disease are similar between αβ-TCR -/- and wild-type C57BL/6 (WT) mice during acute phase of infection [0-13 days post-infection (dpi)]. While WT mice clear infectious virus in the brain by 13 dpi, αβ-TCR -/- maintain infectious virus in the brain to 92 dpi. Persistent brain infection in αβ-TCR -/- correlated with inflammatory infiltrates and elevated cytokine protein levels in the brain at later time points. Persistent brain infection of αβ-TCR -/- mice provides a novel model to study prolonged alphaviral infection as well as the effects and biomarkers of long-term viral inflammation in the brain.
KW - Alphavirus
KW - Immunology
KW - Persistent infection
KW - TC83
KW - VEEV
UR - http://www.scopus.com/inward/record.url?scp=85011949160&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85011949160&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2017.00081
DO - 10.3389/fmicb.2017.00081
M3 - Article
AN - SCOPUS:85011949160
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
IS - JAN
M1 - 81
ER -