Live-cell super-resolution imaging with trimethoprim conjugates

Richard Wombacher; Meike Heidbreder; Sebastian Van De Linde; Michael P. Sheetz; Mike Heilemann; Virginia W. Cornish; Markus Sauer

doi:10.1038/nmeth.1489

Live-cell super-resolution imaging with trimethoprim conjugates

Richard Wombacher, Meike Heidbreder, Sebastian Van De Linde, Michael P. Sheetz, Mike Heilemann, Virginia W. Cornish, Markus Sauer

Research output: Contribution to journal › Article › peer-review

292 Scopus citations

Abstract

The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at ∼20 nm resolution.

Original language	English (US)
Pages (from-to)	717-719
Number of pages	3
Journal	Nature Methods
Volume	7
Issue number	9
DOIs	https://doi.org/10.1038/nmeth.1489
State	Published - Sep 2010
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1489

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title = "Live-cell super-resolution imaging with trimethoprim conjugates",

abstract = "The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at ∼20 nm resolution.",

author = "Richard Wombacher and Meike Heidbreder and {Van De Linde}, Sebastian and Sheetz, {Michael P.} and Mike Heilemann and Cornish, {Virginia W.} and Markus Sauer",

note = "Funding Information: This work was supported by the Biophotonics and the Systems Biology Initiative (Forschungseinheiten der Systembiologie) of the Bundesministerium f{\"u}r Bildung und Forschung and by the US National Institutes of Health (RO1GM54469 and RC1GM091804 to V.W.C. and M.P.S.). R.W. was supported by a Deutscher Akademischer Austausch Dienst fellowship.",

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doi = "10.1038/nmeth.1489",

language = "English (US)",

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AU - Wombacher, Richard

AU - Heidbreder, Meike

AU - Van De Linde, Sebastian

AU - Sheetz, Michael P.

AU - Heilemann, Mike

AU - Cornish, Virginia W.

AU - Sauer, Markus

N1 - Funding Information: This work was supported by the Biophotonics and the Systems Biology Initiative (Forschungseinheiten der Systembiologie) of the Bundesministerium für Bildung und Forschung and by the US National Institutes of Health (RO1GM54469 and RC1GM091804 to V.W.C. and M.P.S.). R.W. was supported by a Deutscher Akademischer Austausch Dienst fellowship.

PY - 2010/9

Y1 - 2010/9

N2 - The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at ∼20 nm resolution.

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Live-cell super-resolution imaging with trimethoprim conjugates

Abstract

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