Live-cell super-resolution imaging with trimethoprim conjugates

Richard Wombacher, Meike Heidbreder, Sebastian Van De Linde, Michael Sheetz, Mike Heilemann, Virginia W. Cornish, Markus Sauer

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at ∼20 nm resolution.

Original languageEnglish (US)
Pages (from-to)717-719
Number of pages3
JournalNature Methods
Volume7
Issue number9
DOIs
StatePublished - Sep 1 2010
Externally publishedYes

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Trimethoprim
Fluorophores
Imaging techniques
Proteins
Optical Imaging
Photons
Histones
Labeling
Labels
Fluorescence
Cells
Fluxes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology
  • Medicine(all)

Cite this

Wombacher, R., Heidbreder, M., Van De Linde, S., Sheetz, M., Heilemann, M., Cornish, V. W., & Sauer, M. (2010). Live-cell super-resolution imaging with trimethoprim conjugates. Nature Methods, 7(9), 717-719. https://doi.org/10.1038/nmeth.1489

Live-cell super-resolution imaging with trimethoprim conjugates. / Wombacher, Richard; Heidbreder, Meike; Van De Linde, Sebastian; Sheetz, Michael; Heilemann, Mike; Cornish, Virginia W.; Sauer, Markus.

In: Nature Methods, Vol. 7, No. 9, 01.09.2010, p. 717-719.

Research output: Contribution to journalArticle

Wombacher, R, Heidbreder, M, Van De Linde, S, Sheetz, M, Heilemann, M, Cornish, VW & Sauer, M 2010, 'Live-cell super-resolution imaging with trimethoprim conjugates', Nature Methods, vol. 7, no. 9, pp. 717-719. https://doi.org/10.1038/nmeth.1489
Wombacher R, Heidbreder M, Van De Linde S, Sheetz M, Heilemann M, Cornish VW et al. Live-cell super-resolution imaging with trimethoprim conjugates. Nature Methods. 2010 Sep 1;7(9):717-719. https://doi.org/10.1038/nmeth.1489
Wombacher, Richard ; Heidbreder, Meike ; Van De Linde, Sebastian ; Sheetz, Michael ; Heilemann, Mike ; Cornish, Virginia W. ; Sauer, Markus. / Live-cell super-resolution imaging with trimethoprim conjugates. In: Nature Methods. 2010 ; Vol. 7, No. 9. pp. 717-719.
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