Live-cell super-resolution imaging with trimethoprim conjugates

Richard Wombacher, Meike Heidbreder, Sebastian Van De Linde, Michael Sheetz, Mike Heilemann, Virginia W. Cornish, Markus Sauer

Research output: Contribution to journalArticle

248 Scopus citations

Abstract

The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at ∼20 nm resolution.

Original languageEnglish (US)
Pages (from-to)717-719
Number of pages3
JournalNature Methods
Volume7
Issue number9
DOIs
StatePublished - Sep 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology
  • Medicine(all)

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  • Cite this

    Wombacher, R., Heidbreder, M., Van De Linde, S., Sheetz, M., Heilemann, M., Cornish, V. W., & Sauer, M. (2010). Live-cell super-resolution imaging with trimethoprim conjugates. Nature Methods, 7(9), 717-719. https://doi.org/10.1038/nmeth.1489