Live offspring produced by mouse oocytes derived from premeiotic fetal germ cells

Wei Shen, Donghui Zhang, Tingting Qing, Jing Cheng, Zhaodai Bai, Yuqiang Shi, Mingxiao Ding, Hongkui Deng

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Mature mouse oocytes currently can be generated in vitro from the primary oocytes of primordial follicles but not from premeiolic fetal germ cells. In this study we established a simple, efficient method that can be used to obtain mature oocytes from the premeiotic germ cells of a fetal mouse 12.5 days postcoitum (dpc). Mouse 12.5-dpc fetal ovaries were transplanted under the kidney capsule of recipient mice to initiate oocyte growth from the premeiotic germ cell stage, and they were recovered after 14 days. Subsequently, the primary and early secondary follicles generated in the ovarian grafts were isolated and cultured for 16 days in vitro. The mature oocytes ovulated from these follicles were able to fertilize in vitro to produce live offspring. We further show that the in vitro fertilization offspring were normal and able to successfully mate with both females and males, and the patterns of the methylated sites of the in vitro mature oocytes were similar to those of normal mice. This is the first report describing premeiotic fetal germ cells able to enter a second meiosis and support embryonic development to term by a combination of in vivo transplantation and in vitro culture. In addition, we have shown that the whole process of oogenesis, from premeiotic germ cells to germinal vesicle (GV)-stage oocytes, can be carried out under the kidney capsule.

Original languageEnglish (US)
Pages (from-to)615-623
Number of pages9
JournalBiology of Reproduction
Volume75
Issue number4
DOIs
StatePublished - Oct 2006
Externally publishedYes

Fingerprint

Germ Cells
Oocytes
Capsules
Kidney
Oogenesis
Meiosis
Fertilization in Vitro
Embryonic Development
Ovary
Transplantation
In Vitro Techniques
Transplants
Growth

Keywords

  • Developmental biology
  • Fetal germ cells
  • Follicular development
  • Meiosis
  • Mouse oogenesis
  • Oocyte
  • Ovary
  • Primordial germ cell development

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Shen, W., Zhang, D., Qing, T., Cheng, J., Bai, Z., Shi, Y., ... Deng, H. (2006). Live offspring produced by mouse oocytes derived from premeiotic fetal germ cells. Biology of Reproduction, 75(4), 615-623. https://doi.org/10.1095/biolreprod.106.051482

Live offspring produced by mouse oocytes derived from premeiotic fetal germ cells. / Shen, Wei; Zhang, Donghui; Qing, Tingting; Cheng, Jing; Bai, Zhaodai; Shi, Yuqiang; Ding, Mingxiao; Deng, Hongkui.

In: Biology of Reproduction, Vol. 75, No. 4, 10.2006, p. 615-623.

Research output: Contribution to journalArticle

Shen, W, Zhang, D, Qing, T, Cheng, J, Bai, Z, Shi, Y, Ding, M & Deng, H 2006, 'Live offspring produced by mouse oocytes derived from premeiotic fetal germ cells', Biology of Reproduction, vol. 75, no. 4, pp. 615-623. https://doi.org/10.1095/biolreprod.106.051482
Shen, Wei ; Zhang, Donghui ; Qing, Tingting ; Cheng, Jing ; Bai, Zhaodai ; Shi, Yuqiang ; Ding, Mingxiao ; Deng, Hongkui. / Live offspring produced by mouse oocytes derived from premeiotic fetal germ cells. In: Biology of Reproduction. 2006 ; Vol. 75, No. 4. pp. 615-623.
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