Local injection of insulin-zinc stimulates DNA synthesis in skin donor site wound

Xiao Jun Zhang, David L. Chinkes, V-M Ramanujam, Robert R. Wolfe

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Our group has reported the ability of local insulin-zinc injection to accelerate skin wound healing. This experiment was conducted to investigate the metabolic mechanisms of locally injected insulin-zinc in accelerating wound healing. A partial thickness skin donor site wound was created on the back, and indwelling catheters were placed in the carotid artery and jugular vein in anesthetized rabbits. On day 7 after injury, the wound was either injected with 0.2 U of insulin-zinc, 0.4 μg zinc (the amount of zinc in 0.2 U of insulin-zinc), or not injected; stable isotope tracers were infused for measurement of wound DNA synthetic rate as a reflection of cell proliferation. Wound protein synthetic and breakdown rates were also measured. The local insulin-zinc injection increased wound insulin concentration from below 5 pmol/L to 1,260±780 pmol/L with minor changes in blood glucose concentration that did not require exogenous glucose replacement. In the insulin-zinc-injected wound, the total DNA synthetic rate was increased by ∼50% (p<0.05 vs. control). In the zinc-injected wound, whereas total DNA synthetic rate was increased (p<0.05 vs. control), net protein deposition (synthesis-breakdown) was less (p<0.05) than those in the control and insulin-zinc groups. We conclude that local insulin-zinc injection stimulates wound DNA synthesis, which would be expected to accelerate wound reepithelialization.

Original languageEnglish (US)
Pages (from-to)258-265
Number of pages8
JournalWound Repair and Regeneration
Volume15
Issue number2
DOIs
StatePublished - Mar 2007

Fingerprint

Zinc
Insulin
Skin
Injections
DNA
Wounds and Injuries
Wound Healing
Indwelling Catheters
Jugular Veins
Carotid Arteries
Isotopes
Blood Glucose
Proteins
Cell Proliferation
Rabbits
Glucose

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Local injection of insulin-zinc stimulates DNA synthesis in skin donor site wound. / Zhang, Xiao Jun; Chinkes, David L.; Ramanujam, V-M; Wolfe, Robert R.

In: Wound Repair and Regeneration, Vol. 15, No. 2, 03.2007, p. 258-265.

Research output: Contribution to journalArticle

Zhang, Xiao Jun ; Chinkes, David L. ; Ramanujam, V-M ; Wolfe, Robert R. / Local injection of insulin-zinc stimulates DNA synthesis in skin donor site wound. In: Wound Repair and Regeneration. 2007 ; Vol. 15, No. 2. pp. 258-265.
@article{faa5708a97ef4c109421e86a232bcd7e,
title = "Local injection of insulin-zinc stimulates DNA synthesis in skin donor site wound",
abstract = "Our group has reported the ability of local insulin-zinc injection to accelerate skin wound healing. This experiment was conducted to investigate the metabolic mechanisms of locally injected insulin-zinc in accelerating wound healing. A partial thickness skin donor site wound was created on the back, and indwelling catheters were placed in the carotid artery and jugular vein in anesthetized rabbits. On day 7 after injury, the wound was either injected with 0.2 U of insulin-zinc, 0.4 μg zinc (the amount of zinc in 0.2 U of insulin-zinc), or not injected; stable isotope tracers were infused for measurement of wound DNA synthetic rate as a reflection of cell proliferation. Wound protein synthetic and breakdown rates were also measured. The local insulin-zinc injection increased wound insulin concentration from below 5 pmol/L to 1,260±780 pmol/L with minor changes in blood glucose concentration that did not require exogenous glucose replacement. In the insulin-zinc-injected wound, the total DNA synthetic rate was increased by ∼50{\%} (p<0.05 vs. control). In the zinc-injected wound, whereas total DNA synthetic rate was increased (p<0.05 vs. control), net protein deposition (synthesis-breakdown) was less (p<0.05) than those in the control and insulin-zinc groups. We conclude that local insulin-zinc injection stimulates wound DNA synthesis, which would be expected to accelerate wound reepithelialization.",
author = "Zhang, {Xiao Jun} and Chinkes, {David L.} and V-M Ramanujam and Wolfe, {Robert R.}",
year = "2007",
month = "3",
doi = "10.1111/j.1524-475X.2007.00213.x",
language = "English (US)",
volume = "15",
pages = "258--265",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Local injection of insulin-zinc stimulates DNA synthesis in skin donor site wound

AU - Zhang, Xiao Jun

AU - Chinkes, David L.

AU - Ramanujam, V-M

AU - Wolfe, Robert R.

PY - 2007/3

Y1 - 2007/3

N2 - Our group has reported the ability of local insulin-zinc injection to accelerate skin wound healing. This experiment was conducted to investigate the metabolic mechanisms of locally injected insulin-zinc in accelerating wound healing. A partial thickness skin donor site wound was created on the back, and indwelling catheters were placed in the carotid artery and jugular vein in anesthetized rabbits. On day 7 after injury, the wound was either injected with 0.2 U of insulin-zinc, 0.4 μg zinc (the amount of zinc in 0.2 U of insulin-zinc), or not injected; stable isotope tracers were infused for measurement of wound DNA synthetic rate as a reflection of cell proliferation. Wound protein synthetic and breakdown rates were also measured. The local insulin-zinc injection increased wound insulin concentration from below 5 pmol/L to 1,260±780 pmol/L with minor changes in blood glucose concentration that did not require exogenous glucose replacement. In the insulin-zinc-injected wound, the total DNA synthetic rate was increased by ∼50% (p<0.05 vs. control). In the zinc-injected wound, whereas total DNA synthetic rate was increased (p<0.05 vs. control), net protein deposition (synthesis-breakdown) was less (p<0.05) than those in the control and insulin-zinc groups. We conclude that local insulin-zinc injection stimulates wound DNA synthesis, which would be expected to accelerate wound reepithelialization.

AB - Our group has reported the ability of local insulin-zinc injection to accelerate skin wound healing. This experiment was conducted to investigate the metabolic mechanisms of locally injected insulin-zinc in accelerating wound healing. A partial thickness skin donor site wound was created on the back, and indwelling catheters were placed in the carotid artery and jugular vein in anesthetized rabbits. On day 7 after injury, the wound was either injected with 0.2 U of insulin-zinc, 0.4 μg zinc (the amount of zinc in 0.2 U of insulin-zinc), or not injected; stable isotope tracers were infused for measurement of wound DNA synthetic rate as a reflection of cell proliferation. Wound protein synthetic and breakdown rates were also measured. The local insulin-zinc injection increased wound insulin concentration from below 5 pmol/L to 1,260±780 pmol/L with minor changes in blood glucose concentration that did not require exogenous glucose replacement. In the insulin-zinc-injected wound, the total DNA synthetic rate was increased by ∼50% (p<0.05 vs. control). In the zinc-injected wound, whereas total DNA synthetic rate was increased (p<0.05 vs. control), net protein deposition (synthesis-breakdown) was less (p<0.05) than those in the control and insulin-zinc groups. We conclude that local insulin-zinc injection stimulates wound DNA synthesis, which would be expected to accelerate wound reepithelialization.

UR - http://www.scopus.com/inward/record.url?scp=33847711880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847711880&partnerID=8YFLogxK

U2 - 10.1111/j.1524-475X.2007.00213.x

DO - 10.1111/j.1524-475X.2007.00213.x

M3 - Article

C2 - 17352759

AN - SCOPUS:33847711880

VL - 15

SP - 258

EP - 265

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 2

ER -