Localization of CaMKIIα in rat primary sensory neurons: Increase in inflammation

Susan M. Carlton

    Research output: Contribution to journalArticle

    46 Citations (Scopus)

    Abstract

    This study investigates Ca2+/calmodulin kinase IIα (CaMKIIα) in primary sensory neurons. Immunohistochemical staining with a CaMKIIα antibody demonstrates 28% of dorsal root ganglion (DRG) cells are positively stained and have a diameter of 27±2.4 μm (mean±S.D.). Placement of tight ligatures around the sciatic nerve demonstrates a build up of immunoreaction product proximal to the ligatures indicating that CaMKIIα is transported into the peripheral processes of DRG cells. Immunostaining of lumbar dorsal roots at the electron microscopic level demonstrates reaction product in 15.4±2.1% of unmyelinated and 2.4±1.0% of myelinated axons, indicating that CaMKIIα is transported into the central processes of DRG cells. Electron microscopic analysis of normal digital nerves demonstrates CaMKIIα labeling in 3.3±0.3% of unmyelinated and 2.0±1.1% of myelinated cutaneous axons. These percentages increase significantly to 14.1±2.3% for unmyelinated and 5.1±1.4% for myelinated axons 48 h after complete Freund's adjuvant-induced inflammation of the hindpaw. The data indicate that CaMKIIα is present in small diameter primary sensory neurons, that it is transported into the peripheral and central processes of these cells and may play a role in processing noxious input, particularly in the inflamed state.

    Original languageEnglish (US)
    Pages (from-to)252-259
    Number of pages8
    JournalBrain Research
    Volume947
    Issue number2
    DOIs
    StatePublished - Aug 30 2002

    Fingerprint

    Calcium-Calmodulin-Dependent Protein Kinases
    Sensory Receptor Cells
    Inflammation
    Spinal Ganglia
    Axons
    Ligation
    Electrons
    Freund's Adjuvant
    Spinal Nerve Roots
    Sciatic Nerve
    Staining and Labeling
    Skin
    Antibodies

    Keywords

    • Calcium
    • Calmodulin

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Clinical Neurology
    • Developmental Biology
    • Molecular Biology

    Cite this

    Localization of CaMKIIα in rat primary sensory neurons : Increase in inflammation. / Carlton, Susan M.

    In: Brain Research, Vol. 947, No. 2, 30.08.2002, p. 252-259.

    Research output: Contribution to journalArticle

    Carlton, Susan M. / Localization of CaMKIIα in rat primary sensory neurons : Increase in inflammation. In: Brain Research. 2002 ; Vol. 947, No. 2. pp. 252-259.
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