Localization of cytoplasmic dynein to mitotic spindles and kinetochores

E. R. Steuer, L. Wordeman, T. A. Schroer, Michael Sheetz

Research output: Contribution to journalArticle

377 Citations (Scopus)

Abstract

WHAT is the origin of the forces generating chromosome and spindle movements in mitosis? Both microtubule dynamics1 and microtubule-dependent motors2 have been proposed as the source of these motor forces. Cytoplasmic dynein and kinesin are two soluble proteins3-6 that power membranous organelle movements on microtubules7,8. Kinesin directs movement of organelles to the 'plus' end of microtubules, and is found at the mitotic spindle in sea urchin embryos6, but not in mammalian cells9. Cytoplasmic dynein translocates organelles to the 'minus' end of microtubules, and is composed of two heavy chains and several light chains3,4. We report here that monoclonal antibodies to two of these subunits and to another polypeptide that associates with dynein localize the protein to the mitotic spindle and to the kinetochores of isolated chromosomes, suggesting that cytoplasmic dynein is important in powering movements of the spindle and chromosomes in dividing cells.

Original languageEnglish (US)
Pages (from-to)266-268
Number of pages3
JournalNature
Volume345
Issue number6272
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

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Cytoplasmic Dyneins
Kinetochores
Spindle Apparatus
Microtubules
Organelles
Kinesin
Chromosomes
Dyneins
Sea Urchins
Mitosis
Monoclonal Antibodies
Light
Peptides
Proteins

ASJC Scopus subject areas

  • General

Cite this

Localization of cytoplasmic dynein to mitotic spindles and kinetochores. / Steuer, E. R.; Wordeman, L.; Schroer, T. A.; Sheetz, Michael.

In: Nature, Vol. 345, No. 6272, 01.01.1990, p. 266-268.

Research output: Contribution to journalArticle

Steuer, ER, Wordeman, L, Schroer, TA & Sheetz, M 1990, 'Localization of cytoplasmic dynein to mitotic spindles and kinetochores', Nature, vol. 345, no. 6272, pp. 266-268. https://doi.org/10.1038/345266a0
Steuer, E. R. ; Wordeman, L. ; Schroer, T. A. ; Sheetz, Michael. / Localization of cytoplasmic dynein to mitotic spindles and kinetochores. In: Nature. 1990 ; Vol. 345, No. 6272. pp. 266-268.
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