Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes

Sanjiv Sur, Dohn G. Glitz, Hirohito Kita, Stephen M. Kujawa, Ellen A. Peterson, Deborah A. Weiler, Gail M. Kephart, Jill M. Wagner, Terry J. George, Gerald J. Gleich, Kristin M. Leiferman

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP) are generally regarded as eosinophil-specific proteins. We tested whether EDN and ECP are present in mature neutrophils. By indirect immunofluorescence, both eosinophils and neutrophils stained with antibodies to EDN and ECP. Lysates of purified (<0.1% eosinophil contamination) neutrophils contained EDN, 112 ± 4 ng/106 cells, and ECP, 163 ± 2 ng/106 cells, whereas eosinophil major basic protein (MBP) was not detectable. Electron microscopic examination of immunogold-labeled buffy coat cells stained with EDN antibody showed that EDN is localized to neutrophil granules. Finally, EDN mRNA was detected in lysates of highly purified neutrophils (0.001% eosinophil contamination) by the reverse transcription- polymerase chain reaction. We conclude that proteins that are either identical to or immunologically cross-reactive with EDN and ECP are present in neutrophils and that EDN is synthesized and localized to neutrophil granules. Thus, caution must be exercised in interpreting the presence of EDN and ECP as specific markers of eosinophil-associated inflammation in human disease.

Original languageEnglish (US)
Pages (from-to)715-722
Number of pages8
JournalJournal of Leukocyte Biology
Volume63
Issue number6
StatePublished - Jun 1998
Externally publishedYes

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Eosinophil-Derived Neurotoxin
Eosinophil Cationic Protein
Leukocytes
Neutrophils
Eosinophils
Eosinophil Major Basic Protein
Glycocalyx
Antibodies
Indirect Fluorescent Antibody Technique
Reverse Transcription
Proteins

Keywords

  • Electron microscopy
  • Immunofluorescence
  • Reverse transcription-polymerase chain reaction

ASJC Scopus subject areas

  • Cell Biology

Cite this

Sur, S., Glitz, D. G., Kita, H., Kujawa, S. M., Peterson, E. A., Weiler, D. A., ... Leiferman, K. M. (1998). Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes. Journal of Leukocyte Biology, 63(6), 715-722.

Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes. / Sur, Sanjiv; Glitz, Dohn G.; Kita, Hirohito; Kujawa, Stephen M.; Peterson, Ellen A.; Weiler, Deborah A.; Kephart, Gail M.; Wagner, Jill M.; George, Terry J.; Gleich, Gerald J.; Leiferman, Kristin M.

In: Journal of Leukocyte Biology, Vol. 63, No. 6, 06.1998, p. 715-722.

Research output: Contribution to journalArticle

Sur, S, Glitz, DG, Kita, H, Kujawa, SM, Peterson, EA, Weiler, DA, Kephart, GM, Wagner, JM, George, TJ, Gleich, GJ & Leiferman, KM 1998, 'Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes', Journal of Leukocyte Biology, vol. 63, no. 6, pp. 715-722.
Sur S, Glitz DG, Kita H, Kujawa SM, Peterson EA, Weiler DA et al. Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes. Journal of Leukocyte Biology. 1998 Jun;63(6):715-722.
Sur, Sanjiv ; Glitz, Dohn G. ; Kita, Hirohito ; Kujawa, Stephen M. ; Peterson, Ellen A. ; Weiler, Deborah A. ; Kephart, Gail M. ; Wagner, Jill M. ; George, Terry J. ; Gleich, Gerald J. ; Leiferman, Kristin M. / Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes. In: Journal of Leukocyte Biology. 1998 ; Vol. 63, No. 6. pp. 715-722.
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AU - Leiferman, Kristin M.

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