Localization of the Fas-Fas ligand system in human fetal membranes

Hassan Harirah, Sahar E. Donia, Vanita Parkash, David C. Jones, Chaur Dong Hsu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

OBJECTIVE: To determine if fetal membranes might be one of the sources of Fas and Fas ligand in amniotic fluid. STUDY DESIGN: Human fetal membranes from elective cesarean section (n = 6) were fixed in paraformaldehyde. Rolls of paraffinembedded fetal membranes were cut into 5-μm sections. After blocking with horse and goat sera, sections were incubated overnight with primary antibodies followed by the appropriate secondary antibodies. Avidin-biotin complex and diaminobenzidine were used for immunoperoxidase localization. Expression of Fas and Fas ligand was read by light microscopy. RESULTS: Both Fas and Fas ligand were localized in amnion, chorion and decidual layers. In amnion, Fas and Fas ligand were expressed predominantly in epithelial cells and fibroblasts, while there was no immunostaining in the subepithelial compact connective tissue. In the chorion, the expression was mainly in the chorionic trophoblast, with inconsistent expression in the reticular layer. In the decidua, the expression of Fas and Fas ligand was less prominent than in amnion and chorion. CONCLUSION: Localization of Fas and Fas ligand in human fetal membranes suggests that fetal membranes could be one of the sources of soluble Fas and Fas ligand in amniotic fluid.

Original languageEnglish (US)
Pages (from-to)611-616
Number of pages6
JournalJournal of Reproductive Medicine for the Obstetrician and Gynecologist
Volume47
Issue number8
StatePublished - Aug 2002

Fingerprint

Extraembryonic Membranes
Fas Ligand Protein
Chorion
Amnion
Amniotic Fluid
Decidua
Avidin
Antibodies
Trophoblasts
Biotin
Goats
Cesarean Section
Connective Tissue
Horses
Microscopy
Fibroblasts
Epithelial Cells
Light
Serum

Keywords

  • Amniotic fluid
  • Apoptosis
  • Fas antigens
  • Fetal membranes
  • Ligands

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Localization of the Fas-Fas ligand system in human fetal membranes. / Harirah, Hassan; Donia, Sahar E.; Parkash, Vanita; Jones, David C.; Hsu, Chaur Dong.

In: Journal of Reproductive Medicine for the Obstetrician and Gynecologist, Vol. 47, No. 8, 08.2002, p. 611-616.

Research output: Contribution to journalArticle

Harirah, Hassan ; Donia, Sahar E. ; Parkash, Vanita ; Jones, David C. ; Hsu, Chaur Dong. / Localization of the Fas-Fas ligand system in human fetal membranes. In: Journal of Reproductive Medicine for the Obstetrician and Gynecologist. 2002 ; Vol. 47, No. 8. pp. 611-616.
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