Localized colonic stem cell transplantation enhances tissue regeneration in murine colitis

Qiqi Zhou, Donald D. Price, Kara L. Dreher, Barry Pronold, Christopher S. Callam, Jay Sharma, G. Nicholas Verne

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Many patients suffer from chronic gastrointestinal diseases characterized by chronic inflammation, increased intestinal permeability and visceral pain in which there is no definitive treatment. Adult stem cells have recently been used in various disease states to contribute wound-healing processes. In the current study we investigated the ability of intra-colonic adult stem cells application to heal colonic inflammation in IL-10-/- mice with active colitis. The aims of this study were to determine whether intra-colonic infusion of adult colonic stem cells (CSCs) (local stem cell transplantation): (i) restores intestinal permeability; (ii) attenuates visceral hypersensitivity; (iii) heals murine colitis. IL-10-/- mice with active colitis were transplanted with adult stem cells. Mice received either a single intracolonic infusion of CSCs or colonic epithelial cells. Two weeks after transplantation, we measured visceral hypersensitivity and intestinal permeability and correlated these with histological improvement of colitis. IL-10-/- mice that received stem cell transplantation showed histopathologic evidence of recovery from colitis. Improvement in colitis as graded by pathology scores correlated with restoration of intestinal permeability and decreased visceral hypersensitivity. Intra-colonic administration of CSCs is a potential therapeutic method for treating refractory symptoms in patients with chronic gastrointestinal diseases associated with chronic inflammation and visceral hypersensitivity. This method may be safer and should have far fewer side effects than systemic stem cell administration.

Original languageEnglish (US)
Pages (from-to)1900-1915
Number of pages16
JournalJournal of Cellular and Molecular Medicine
Issue number8
StatePublished - Aug 2012
Externally publishedYes


  • Cytokines
  • IL-10 mice
  • Inflammatory bowel disease (IBD)
  • Intestinal permeability
  • Stem cell
  • TNF-α and IFN-γ
  • Visceral hypersensitivity

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology


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