Abstract
Rat liver nuclei contain a 29-nucleotides-long RNA (fr 3-RNA) which is transcribed from middle repetitive DNA sequences. By Southern analysis of restriction fragments of rat albumin and α-fetoprotein genomic clones, DNA sequences complementary to this RNA were detected on a 4.6 kbp EcoRI fragment located 600 bp downstream from the termination exon of the albumin gene and on a 2 kbp EcoRI-HindIII fragment located 10 kbp downstream from the restriction fragment containing the α-fetoprotein site. No sequence complementary to this RNA was found either in the introns of exons of both genes or in the regions extending 7 kbp upstream from the first albumin exon and 10 kbp upstream of the first α-fetoprotein exon. We concluded that sequences complementary to fr 3-RNA are present at the 3′-end flanking regions of the rat albumin and α-fetoprotein gene complexes.
Original language | English (US) |
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Pages (from-to) | 164-169 |
Number of pages | 6 |
Journal | BBA - Gene Structure and Expression |
Volume | 782 |
Issue number | 2 |
DOIs | |
State | Published - Jun 16 1984 |
Keywords
- (Rat liver)
- Albumin gene
- Repetitive sequence
- fr 3-RNA
- α-Fetoprotein gene
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics