Abstract
Vaccinia virus (VACV) has been associated with several bovine vaccinia outbreaks in Brazil, causing exanthematic lesions in dairy cattle and humans. The way that VACV circulates in the environment is unknown, as is the way that this virus is transferred from wildlife to farms. Rodents are hypothetical VACV reservoirs, and murine feces has been identified as a potential source of viral shedding and transmission. In this work, we analyzed the stability of VACV infectious particles and DNA in feces from intranasally infected mice, exposed to environmental temperature and humidity, by titration assays and PCR, respectively. The results showed that VACV infectious particles were still detected at 20 days post-environmental-exposure (d.p.e.), while viral DNA was detected until 60 d.p.e. A gradual decrease in fecal viral load could be detected in all analyzed VACV strains. This work indicates long-lasting stability of VACV in murine feces and reinforces the idea that fecal matter may represent a potential source of circulating virus among rodents.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1551-1553 |
| Number of pages | 3 |
| Journal | Archives of virology |
| Volume | 154 |
| Issue number | 9 |
| DOIs | |
| State | Published - Jan 1 2009 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
Cite this
Long-lasting stability of Vaccinia virus strains in murine feces : implications for virus circulation and environmental maintenance. / Abrahão, Jônatas S.; Trindade, Giliane de Souza; Ferreira, Jaqueline M.Siqueira; Kroon Campos, Rafael; Bonjardim, Cláudio A.; Ferreira, Paulo C.Peregrino; Kroon, Erna Geessien.
In: Archives of virology, Vol. 154, No. 9, 01.01.2009, p. 1551-1553.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Long-lasting stability of Vaccinia virus strains in murine feces
T2 - implications for virus circulation and environmental maintenance.
AU - Abrahão, Jônatas S.
AU - Trindade, Giliane de Souza
AU - Ferreira, Jaqueline M.Siqueira
AU - Kroon Campos, Rafael
AU - Bonjardim, Cláudio A.
AU - Ferreira, Paulo C.Peregrino
AU - Kroon, Erna Geessien
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Vaccinia virus (VACV) has been associated with several bovine vaccinia outbreaks in Brazil, causing exanthematic lesions in dairy cattle and humans. The way that VACV circulates in the environment is unknown, as is the way that this virus is transferred from wildlife to farms. Rodents are hypothetical VACV reservoirs, and murine feces has been identified as a potential source of viral shedding and transmission. In this work, we analyzed the stability of VACV infectious particles and DNA in feces from intranasally infected mice, exposed to environmental temperature and humidity, by titration assays and PCR, respectively. The results showed that VACV infectious particles were still detected at 20 days post-environmental-exposure (d.p.e.), while viral DNA was detected until 60 d.p.e. A gradual decrease in fecal viral load could be detected in all analyzed VACV strains. This work indicates long-lasting stability of VACV in murine feces and reinforces the idea that fecal matter may represent a potential source of circulating virus among rodents.
AB - Vaccinia virus (VACV) has been associated with several bovine vaccinia outbreaks in Brazil, causing exanthematic lesions in dairy cattle and humans. The way that VACV circulates in the environment is unknown, as is the way that this virus is transferred from wildlife to farms. Rodents are hypothetical VACV reservoirs, and murine feces has been identified as a potential source of viral shedding and transmission. In this work, we analyzed the stability of VACV infectious particles and DNA in feces from intranasally infected mice, exposed to environmental temperature and humidity, by titration assays and PCR, respectively. The results showed that VACV infectious particles were still detected at 20 days post-environmental-exposure (d.p.e.), while viral DNA was detected until 60 d.p.e. A gradual decrease in fecal viral load could be detected in all analyzed VACV strains. This work indicates long-lasting stability of VACV in murine feces and reinforces the idea that fecal matter may represent a potential source of circulating virus among rodents.
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UR - http://www.scopus.com/inward/citedby.url?scp=70449701615&partnerID=8YFLogxK
U2 - 10.1007/s00705-009-0470-1
DO - 10.1007/s00705-009-0470-1
M3 - Article
C2 - 19710997
AN - SCOPUS:70449701615
VL - 154
SP - 1551
EP - 1553
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 9
ER -