Long-Term Skeletal Muscle Mitochondrial Dysfunction is Associated with Hypermetabolism in Severely Burned Children

Craig Porter, David Herndon, Elisabet Børsheim, Nisha Bhattarai, Tony Chao, Paul T. Reidy, Blake Rasmussen, Clark R. Andersen, Oscar Suman, Labros S. Sidossis

Research output: Contribution to journalArticle

20 Scopus citations


The long-term impact of burn trauma on skeletal muscle bioenergetics remains unknown. Here, the authors determined respiratory capacity and function of skeletal muscle mitochondria in healthy individuals and in burn victims for up to 2 years postinjury. Hypermetabolism was determined by the difference in predicted and measured metabolic rate. Biopsies were collected from the m. vastus lateralis of 16 healthy men (26 ± 4 years) and 69 children (8 ± 5 years) with burns encompassing ≥30% of their total BSA. Seventy-nine biopsies were collected from cohorts of burn victims at 2 weeks (n = 18), 6 months (n = 18), 12 months (n = 25), and 24 months (n = 18) postburn. Mitochondrial respiration was determined in saponin-permeabilized myofiber bundles. Outcomes were modeled by analysis of variance, with differences in groups assessed by Tukey-adjusted contrasts. Burn patients were hypermetabolic for up to 2 years postinjury. Coupled mitochondrial respiration was lower at 2 weeks (17 (8) pmol/sec/mg; P <.001), 6 months (41 (30) pmol/sec/mg; P = .03), and 12 months (35 (14) pmol/sec/mg; P <.001) postburn compared with healthy controls (58 (13) pmol/sec/mg). Coupled respiration was greater at 6, 12, and 24 months postburn vs 2 weeks postburn (P <.001). Mitochondrial adenosine diphosphatase and oligomycin sensitivity (measures of coupling control) were lower at all time-points postburn vs control (P <.05), but greater at 6, 12, and 24 months postburn vs 2 weeks postburn (P <.05). Muscle mitochondrial respiratory capacity remains significantly lower in burn victims for 1-year postinjury. Mitochondrial coupling control is diminished for up to 2 years postinjury in burn victims, resulting in greater mitochondrial thermogenesis. These quantitative and qualitative derangements in skeletal muscle bioenergetics likely contribute to the long-term pathophysiological stress response to burn trauma.

Original languageEnglish (US)
JournalJournal of Burn Care and Research
StateAccepted/In press - Sep 7 2015


ASJC Scopus subject areas

  • Emergency Medicine
  • Rehabilitation
  • Surgery

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