Long-term survival of New Zealand rabbit haemorrhagic disease virus RNA in wild rabbits, revealed by RT-PCR and phylogenetic analysis

Naomi L. Forrester, B. Boag, S. R. Moss, S. L. Turner, R. C. Trout, P. J. White, P. J. Hudson, E. A. Gould

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Because Rabbit haemorrhagic disease virus (RHDV) is highly pathogenic for rabbits, farmers illegally introduced it as a bio-control agent onto New Zealand farms in 1997. The virus was dispersed rapidly, initially causing high fatality rates in rabbits. Nevertheless, many survived and these surviving rabbits have been investigated for evidence of infection by RHDV. Livers from healthy rabbits contained RHDV-specific RNA, as shown by nested RT-PCR sequencing. The sequences of the viral capsids were related closely to the released Czech strain of RHDV, although the sequence from one rabbit was related most closely to a Spanish strain of RHDV. Phylogenetic analysis of the capsid sequences of 38 samples implied that there have been at least two introductions of the Czech virus into New Zealand, probably corresponding firstly to the original illegal introduction by farmers and secondly to the introduction of the same virus under governmental control. Genomic length sequence of two samples was obtained, suggesting that they may have retained the potential to be infectious, although this has not yet been demonstrated. The detection of genomic-length RNA in the liver of healthy rabbits suggests that even though a highly virulent virus was introduced into New Zealand, it rapidly established persistent or latent infections in a proportion of rabbits. This might account for their ability to survive in the face of virulent released virus. Moreover, the co-circulation of other strains of RHDV in the same rabbit population, such as the Spanish strain, might also impact on their susceptibility to the bio-control agent.

Original languageEnglish (US)
Pages (from-to)3079-3086
Number of pages8
JournalJournal of General Virology
Volume84
Issue number11
DOIs
StatePublished - Nov 2003
Externally publishedYes

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Rabbit Haemorrhagic Disease Virus
RNA
Rabbits
Polymerase Chain Reaction
Viruses
New Zealand
Capsid
Liver
Infection
Sequence Analysis

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Forrester, N. L., Boag, B., Moss, S. R., Turner, S. L., Trout, R. C., White, P. J., ... Gould, E. A. (2003). Long-term survival of New Zealand rabbit haemorrhagic disease virus RNA in wild rabbits, revealed by RT-PCR and phylogenetic analysis. Journal of General Virology, 84(11), 3079-3086. https://doi.org/10.1099/vir.0.19213-0

Long-term survival of New Zealand rabbit haemorrhagic disease virus RNA in wild rabbits, revealed by RT-PCR and phylogenetic analysis. / Forrester, Naomi L.; Boag, B.; Moss, S. R.; Turner, S. L.; Trout, R. C.; White, P. J.; Hudson, P. J.; Gould, E. A.

In: Journal of General Virology, Vol. 84, No. 11, 11.2003, p. 3079-3086.

Research output: Contribution to journalArticle

Forrester, NL, Boag, B, Moss, SR, Turner, SL, Trout, RC, White, PJ, Hudson, PJ & Gould, EA 2003, 'Long-term survival of New Zealand rabbit haemorrhagic disease virus RNA in wild rabbits, revealed by RT-PCR and phylogenetic analysis', Journal of General Virology, vol. 84, no. 11, pp. 3079-3086. https://doi.org/10.1099/vir.0.19213-0
Forrester, Naomi L. ; Boag, B. ; Moss, S. R. ; Turner, S. L. ; Trout, R. C. ; White, P. J. ; Hudson, P. J. ; Gould, E. A. / Long-term survival of New Zealand rabbit haemorrhagic disease virus RNA in wild rabbits, revealed by RT-PCR and phylogenetic analysis. In: Journal of General Virology. 2003 ; Vol. 84, No. 11. pp. 3079-3086.
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