Abstract
Background: Removal of the uterine luminal epithelium and its basement membrane is necessary for successful implantation of invasive blastocysts. Few reports, however, have specifically addressed the penetration and loss of the uterine luminal epithelial basement membrane (UEBM). We investigated the loss of UEBM by examining the distribution of laminin and type IV collagen. Methods: Blastocyst implantation sites were collected from mice on days 5, 6, and 7 of pregnancy. Paraffin sections were prepared from these tissues and processed with standard immunoperoxidase techniques to reveal the distribution of laminin and type IV collagen. Results: On day 5 of pregnancy blastocysts were adherent to the uterine epithelium. The epithelium and UEBM were complete and uninterrupted. On day 0 the juxtaembryonic uterine epithelium was lost and focal discontinuities were seen along the UEBM. By 1200 hr the UEBM had receded to the region near the ectoplacental cone, but staining was reduced for both antigens over the entire region surrounded by decidual cells. This decreased staining of the UEBM occurred in areas not yet occupied by trophoblast cells. On day 7 the UEBM was lost over the entire embryonic half of the uterine lining, corresponding to the distribution of decidual cells. Conclusions: Progressive loss of the UEBM occurred in a consistent spatiotemporal pattern following the onset of blastocyst implantation. Diminished immunoreactivity of laminin and type IV collagen in the UEBM was closely correlated with the area occupied by decidualized endometrial stroma and occurred in areas not yet in contact with trophoblast cells. We conclude that decidual cells are instrumental in the removal of UEBM during early pregnancy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 27-36 |
| Number of pages | 10 |
| Journal | Anatomical Record |
| Volume | 243 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1995 |
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Keywords
- Basement membrane
- Blastocyst
- Implantation
- Laminin
- Mouse
- Type IV collagen
- Uterus
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Anatomy
Cite this
Loss of laminin and type IV collagen in uterine luminal epithelial basement membranes during blastocyst implantation in the mouse. / Blankenship, T. N.; Given, R. L.
In: Anatomical Record, Vol. 243, No. 1, 1995, p. 27-36.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Loss of laminin and type IV collagen in uterine luminal epithelial basement membranes during blastocyst implantation in the mouse
AU - Blankenship, T. N.
AU - Given, R. L.
PY - 1995
Y1 - 1995
N2 - Background: Removal of the uterine luminal epithelium and its basement membrane is necessary for successful implantation of invasive blastocysts. Few reports, however, have specifically addressed the penetration and loss of the uterine luminal epithelial basement membrane (UEBM). We investigated the loss of UEBM by examining the distribution of laminin and type IV collagen. Methods: Blastocyst implantation sites were collected from mice on days 5, 6, and 7 of pregnancy. Paraffin sections were prepared from these tissues and processed with standard immunoperoxidase techniques to reveal the distribution of laminin and type IV collagen. Results: On day 5 of pregnancy blastocysts were adherent to the uterine epithelium. The epithelium and UEBM were complete and uninterrupted. On day 0 the juxtaembryonic uterine epithelium was lost and focal discontinuities were seen along the UEBM. By 1200 hr the UEBM had receded to the region near the ectoplacental cone, but staining was reduced for both antigens over the entire region surrounded by decidual cells. This decreased staining of the UEBM occurred in areas not yet occupied by trophoblast cells. On day 7 the UEBM was lost over the entire embryonic half of the uterine lining, corresponding to the distribution of decidual cells. Conclusions: Progressive loss of the UEBM occurred in a consistent spatiotemporal pattern following the onset of blastocyst implantation. Diminished immunoreactivity of laminin and type IV collagen in the UEBM was closely correlated with the area occupied by decidualized endometrial stroma and occurred in areas not yet in contact with trophoblast cells. We conclude that decidual cells are instrumental in the removal of UEBM during early pregnancy.
AB - Background: Removal of the uterine luminal epithelium and its basement membrane is necessary for successful implantation of invasive blastocysts. Few reports, however, have specifically addressed the penetration and loss of the uterine luminal epithelial basement membrane (UEBM). We investigated the loss of UEBM by examining the distribution of laminin and type IV collagen. Methods: Blastocyst implantation sites were collected from mice on days 5, 6, and 7 of pregnancy. Paraffin sections were prepared from these tissues and processed with standard immunoperoxidase techniques to reveal the distribution of laminin and type IV collagen. Results: On day 5 of pregnancy blastocysts were adherent to the uterine epithelium. The epithelium and UEBM were complete and uninterrupted. On day 0 the juxtaembryonic uterine epithelium was lost and focal discontinuities were seen along the UEBM. By 1200 hr the UEBM had receded to the region near the ectoplacental cone, but staining was reduced for both antigens over the entire region surrounded by decidual cells. This decreased staining of the UEBM occurred in areas not yet occupied by trophoblast cells. On day 7 the UEBM was lost over the entire embryonic half of the uterine lining, corresponding to the distribution of decidual cells. Conclusions: Progressive loss of the UEBM occurred in a consistent spatiotemporal pattern following the onset of blastocyst implantation. Diminished immunoreactivity of laminin and type IV collagen in the UEBM was closely correlated with the area occupied by decidualized endometrial stroma and occurred in areas not yet in contact with trophoblast cells. We conclude that decidual cells are instrumental in the removal of UEBM during early pregnancy.
KW - Basement membrane
KW - Blastocyst
KW - Implantation
KW - Laminin
KW - Mouse
KW - Type IV collagen
KW - Uterus
UR - http://www.scopus.com/inward/record.url?scp=0029115822&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029115822&partnerID=8YFLogxK
U2 - 10.1002/ar.1092430105
DO - 10.1002/ar.1092430105
M3 - Article
VL - 243
SP - 27
EP - 36
JO - Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology
JF - Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology
SN - 0003-276X
IS - 1
ER -