Loss of the ciliary inhibitory effect of the cystic fibrosis factor following proteolytic digestion and heat denaturation

D. R. Barnett, A. Kurosky, B. H. Bowman, S. C. Barranco

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    The ciliary inhibitor synthesized by fibroblasts derived from cystic fibrosis patients was subjected to proteolytic digestion and heat denaturation. The fraction from fibroblast media containing the ciliary inhibitor was obtained from chromatography on DEAE at pH 8.5. Following digestion with pepsin at pH 3.9 and with papain at pH 8.5, the media fraction no longer inhibited ciliary activity on oyster gills. Experiments were conducted so that cleaved fragments produced by proteolysis or chemical disruption would not be lost. Following exposure to heat, the media fraction failed to inhibit ciliary activity. These data provide additional evidence that the cystic fibrosis ciliary inhibitor is a protein or a protein complex.

    Original languageEnglish (US)
    Pages (from-to)697-701
    Number of pages5
    JournalTexas Reports on Biology and Medicine
    Volume31
    Issue number4
    StatePublished - 1973

    Fingerprint

    Cystic Fibrosis
    Digestion
    Hot Temperature
    Fibroblasts
    Ostreidae
    Papain
    Pepsin A
    Proteolysis
    Chromatography
    Proteins

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    Loss of the ciliary inhibitory effect of the cystic fibrosis factor following proteolytic digestion and heat denaturation. / Barnett, D. R.; Kurosky, A.; Bowman, B. H.; Barranco, S. C.

    In: Texas Reports on Biology and Medicine, Vol. 31, No. 4, 1973, p. 697-701.

    Research output: Contribution to journalArticle

    Barnett, D. R. ; Kurosky, A. ; Bowman, B. H. ; Barranco, S. C. / Loss of the ciliary inhibitory effect of the cystic fibrosis factor following proteolytic digestion and heat denaturation. In: Texas Reports on Biology and Medicine. 1973 ; Vol. 31, No. 4. pp. 697-701.
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