Low and High Km Forms of R;Regular Article Dinitrophenylglutathione-stimulated ATPase in Bovine Lens

Kshama Kumari, Naseem H. Ansari, Manju Saxena, Yogesh C. Awasthi, Satish K. Srivastava

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Dinitrophenyl S-glutathione (Dnp-SG) ATPase which catalyses the hydrolysis of ATP in the presence of GSH-conjugates has been implicated previously in the transport of these conjugates. In the present studies we demonstrate that Dnp-SG ATPase is present in bovine lens epithelium and cortex. The specific activity per mg membrane protein was found to be 75-fold higher in the epithelium as compared to the cortex. No enzyme was detected in the nuclear region of the lens. Dnp-SG ATPase was purified from bovine lens epithelium and cortex using Dnp-SG-Sepharose 6MB affinity chromatography. The partially purified Dnp-SG ATPase had two distinct Km values, 120 μM and 1·0 mM. The antibodies raised against human erythrocyte Dnp-SG ATPase cross-reacted with the bovine lens epithelium Dnp-SG ATPase which was identified by Western blot as a band corresponding to an approximate Mr value of 80000 Da.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalExperimental Eye Research
Volume57
Issue number2
DOIs
StatePublished - Aug 1993

Keywords

  • Bovine lens
  • Dnp-SG ATPase
  • Glutathione
  • Glutathione conjugates
  • Xenobiotic transport

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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