Low and High Km Forms of R;Regular Article Dinitrophenylglutathione-stimulated ATPase in Bovine Lens

Kshama Kumari, Naseem Ansari, Manju Saxena, Yogesh C. Awasthi, Satish Srivastava

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dinitrophenyl S-glutathione (Dnp-SG) ATPase which catalyses the hydrolysis of ATP in the presence of GSH-conjugates has been implicated previously in the transport of these conjugates. In the present studies we demonstrate that Dnp-SG ATPase is present in bovine lens epithelium and cortex. The specific activity per mg membrane protein was found to be 75-fold higher in the epithelium as compared to the cortex. No enzyme was detected in the nuclear region of the lens. Dnp-SG ATPase was purified from bovine lens epithelium and cortex using Dnp-SG-Sepharose 6MB affinity chromatography. The partially purified Dnp-SG ATPase had two distinct Km values, 120 μM and 1·0 mM. The antibodies raised against human erythrocyte Dnp-SG ATPase cross-reacted with the bovine lens epithelium Dnp-SG ATPase which was identified by Western blot as a band corresponding to an approximate Mr value of 80000 Da.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalExperimental Eye Research
Volume57
Issue number2
DOIs
StatePublished - Aug 1993

Fingerprint

Lenses
Adenosine Triphosphatases
Epithelium
Affinity Chromatography
Glutathione
S-(dinitrophenyl)glutathione ATPase
Membrane Proteins
Hydrolysis
Adenosine Triphosphate
Erythrocytes
Western Blotting
Antibodies
Enzymes

Keywords

  • bovine lens
  • Dnp-SG ATPase
  • glutathione
  • glutathione conjugates
  • xenobiotic transport

ASJC Scopus subject areas

  • Sensory Systems
  • Ophthalmology

Cite this

Low and High Km Forms of R;Regular Article Dinitrophenylglutathione-stimulated ATPase in Bovine Lens. / Kumari, Kshama; Ansari, Naseem; Saxena, Manju; Awasthi, Yogesh C.; Srivastava, Satish.

In: Experimental Eye Research, Vol. 57, No. 2, 08.1993, p. 243-247.

Research output: Contribution to journalArticle

Kumari, Kshama ; Ansari, Naseem ; Saxena, Manju ; Awasthi, Yogesh C. ; Srivastava, Satish. / Low and High Km Forms of R;Regular Article Dinitrophenylglutathione-stimulated ATPase in Bovine Lens. In: Experimental Eye Research. 1993 ; Vol. 57, No. 2. pp. 243-247.
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