Low-ionic-strength saline solution-antiglobulin test (LISS-AGT)

LeeAnn Walker

Research output: Contribution to journalArticle

Abstract

CONCLUSIONS: The use of low-ionic-strength saline (LISS) solution as an enhancement for antibody screening and crossmatching was first described by Löw and Messeter in 1974. This method allowed for a reduced incubation time while maintaining adequate specificity and sensitivity of the antiglobulin test (AGT). Since then, the LISS-AGT tube method has been widely used in antibody detection and identification, as well as compatibility testing. As initially described, the method used red blood cells suspended in LISS. Modifications of the method led to development of the commercially prepared LISS additive solutions in use today. The LISS-AGT can be used effectively to detect alloantibodies of all major blood groups in antibody detection, antibody identification, and crossmatching procedures.

Original languageEnglish (US)
Pages (from-to)57-60
Number of pages4
JournalImmunohematology
Volume34
Issue number2
StatePublished - Jun 1 2018

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Coombs Test
Sodium Chloride
Osmolar Concentration
Antibodies
Isoantibodies
Blood Group Antigens
Erythrocytes
Sensitivity and Specificity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology

Cite this

Low-ionic-strength saline solution-antiglobulin test (LISS-AGT). / Walker, LeeAnn.

In: Immunohematology, Vol. 34, No. 2, 01.06.2018, p. 57-60.

Research output: Contribution to journalArticle

Walker, LeeAnn. / Low-ionic-strength saline solution-antiglobulin test (LISS-AGT). In: Immunohematology. 2018 ; Vol. 34, No. 2. pp. 57-60.
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