LPS-Induced Macrophage Activation and Plasma Membrane Fluidity Changes are Inhibited Under Oxidative Stress

Carlos De La Haba Fonteboa, Antoni Morros, Paz Martínez, José R. Palacio

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Macrophage activation is essential for a correct and efficient response of innate immunity. During oxidative stress membrane receptors and/or membrane lipid dynamics can be altered, leading to dysfunctional cell responses. Our aim is to analyze membrane fluidity modifications and cell function under oxidative stress in LPS-activated macrophages. Membrane fluidity of individual living THP-1 macrophages was evaluated by the technique two-photon microscopy. LPS-activated macrophage function was determined by TNFα secretion. It was shown that LPS activation causes fluidification of macrophage plasma membrane and production of TNFα. However, oxidative stress induces rigidification of macrophage plasma membrane and inhibition of cell activation, which is evidenced by a decrease of TNFα secretion. Thus, under oxidative conditions macrophage proinflammatory response might develop in an inefficient manner.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalJournal of Membrane Biology
DOIs
StateAccepted/In press - Sep 12 2016
Externally publishedYes

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Keywords

  • Laurdan
  • Lipid rafts
  • Macrophages
  • Membrane fluidity
  • Oxidative stress
  • TLR4

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

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