Abstract
Lanthanide chelates are a somewhat unique class of molecules that have proven to be useful in the biomedical field due to their extremely large Stokes' shift and long fluorescent lifetimes. The ability of these molecules to produce fluorescence in the low- or zero-back-ground regime makes this class of molecules excellent candidates for use as contrast agents for a wide variety of applications in biological settings. Here we present the preparation, spectroscopic characterization, and application of a new terbium chelate contrast agent, based on the 1,4,7,10-tetraazacyclododecane macrocycle (cyclen), for detection of early-stage malignant lesions in the Syrian hamster cheek pouch. Tb-P(CTMB) delivers bright blue-green luminescence when excited with low photon fiuxes of UV light. As a pilot study, the DMBA-treated Golden Hamster Cheek pouch epithelial cancer model was employed and Tb-P(CTMB) was used as a topical agent for the visual detection of diseased tissue. In this preliminary study the agent tended to associate with early-stage malignant lesions, suggesting that Tb-P(CTMB) could be used as a contrast agent to aid in identifying early-stage oral cancer lesions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1216-1222 |
| Number of pages | 7 |
| Journal | Applied Spectroscopy |
| Volume | 57 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2003 |
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Keywords
- Contrast agents
- Fluorescence imaging
- Lanthanide chelates
- Oral cancer
- Terbium
ASJC Scopus subject areas
- Spectroscopy
- Instrumentation
Cite this
Luminescent Lanthanide Chelate Contrast Agents and Detection of Lesions in the Hamster Oral Cancer Model. / Bornhop, Darryl J.; Griffin, John M M; Goebel, Timothy S.; Sudduth, Mark R.; Bell, Brent; Motamedi, Massoud.
In: Applied Spectroscopy, Vol. 57, No. 10, 10.2003, p. 1216-1222.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Luminescent Lanthanide Chelate Contrast Agents and Detection of Lesions in the Hamster Oral Cancer Model
AU - Bornhop, Darryl J.
AU - Griffin, John M M
AU - Goebel, Timothy S.
AU - Sudduth, Mark R.
AU - Bell, Brent
AU - Motamedi, Massoud
PY - 2003/10
Y1 - 2003/10
N2 - Lanthanide chelates are a somewhat unique class of molecules that have proven to be useful in the biomedical field due to their extremely large Stokes' shift and long fluorescent lifetimes. The ability of these molecules to produce fluorescence in the low- or zero-back-ground regime makes this class of molecules excellent candidates for use as contrast agents for a wide variety of applications in biological settings. Here we present the preparation, spectroscopic characterization, and application of a new terbium chelate contrast agent, based on the 1,4,7,10-tetraazacyclododecane macrocycle (cyclen), for detection of early-stage malignant lesions in the Syrian hamster cheek pouch. Tb-P(CTMB) delivers bright blue-green luminescence when excited with low photon fiuxes of UV light. As a pilot study, the DMBA-treated Golden Hamster Cheek pouch epithelial cancer model was employed and Tb-P(CTMB) was used as a topical agent for the visual detection of diseased tissue. In this preliminary study the agent tended to associate with early-stage malignant lesions, suggesting that Tb-P(CTMB) could be used as a contrast agent to aid in identifying early-stage oral cancer lesions.
AB - Lanthanide chelates are a somewhat unique class of molecules that have proven to be useful in the biomedical field due to their extremely large Stokes' shift and long fluorescent lifetimes. The ability of these molecules to produce fluorescence in the low- or zero-back-ground regime makes this class of molecules excellent candidates for use as contrast agents for a wide variety of applications in biological settings. Here we present the preparation, spectroscopic characterization, and application of a new terbium chelate contrast agent, based on the 1,4,7,10-tetraazacyclododecane macrocycle (cyclen), for detection of early-stage malignant lesions in the Syrian hamster cheek pouch. Tb-P(CTMB) delivers bright blue-green luminescence when excited with low photon fiuxes of UV light. As a pilot study, the DMBA-treated Golden Hamster Cheek pouch epithelial cancer model was employed and Tb-P(CTMB) was used as a topical agent for the visual detection of diseased tissue. In this preliminary study the agent tended to associate with early-stage malignant lesions, suggesting that Tb-P(CTMB) could be used as a contrast agent to aid in identifying early-stage oral cancer lesions.
KW - Contrast agents
KW - Fluorescence imaging
KW - Lanthanide chelates
KW - Oral cancer
KW - Terbium
UR - http://www.scopus.com/inward/record.url?scp=0242301176&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0242301176&partnerID=8YFLogxK
U2 - 10.1366/000370203769699063
DO - 10.1366/000370203769699063
M3 - Article
C2 - 14639748
AN - SCOPUS:0242301176
VL - 57
SP - 1216
EP - 1222
JO - Applied Spectroscopy
JF - Applied Spectroscopy
SN - 0003-7028
IS - 10
ER -