Lung injury after hepatoenteric ischemia-reperfusion: Role of xanthine oxidase

Vance G. Nielsen, Sidhartha Tan, Abraham Weinbroum, Andrew T. Mccammon, Paul N. Samuelson, Simon Gelman, Dale A. Parks

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Oxidant stress plays a major role in the pathophysiologic processes associated with ischemia-reperfusion injury. Xanthine oxidase (XO) is often implicated as a significant source of oxidants and increases in the circulation after hepatoenteric ischemia-reperfusion. We hypothesized that pulmonary injury is associated with hepatic ischemia-reperfusion resulting from descending thoracic aorta occlusion-reperfusion (AoOR). We also proposed that this remote pulmonary injury is attenuated through inactivation of circulating and tissue XO by tungstate, implicating an XO-dependent mechanism. Aortic occlusion was established in rabbits (standard or tungstate diet) for 40 min by 2 h reperfusion. Sham operated rabbits (standard or tungstate diet) served as controls. Hepatic reperfusion injury, as manifested by release of the hepatocellular enzyme alanine aminotransferase (ALT), was markedly increased after AoOR. Suprarenal-intrahepatic occlusion failed to increase ALT release. Tungstate pretreatment significantly (p < 0.05) reduced XO activity and ameliorated liver and intestinal injury (p < 0.05). Lung injury, manifested by increased bronchoalveolar lavage (BAL) protein concentration, BAL lactate dehydrogenase (LDH) activity and increased lung edema was significantly associated with liver injury (p < 0.05) and circulating XO activity (p < 0.001). XO inactivation significantly decreased BAL protein concentration, BAL LDH activity, and lung edema (p < 0.05). We conclude that remote pulmonary injury is significantly influenced by the extent of liver injury and circulating XO activity.

Original languageEnglish (US)
Pages (from-to)1364-1369
Number of pages6
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume154
Issue number5
StatePublished - 1996
Externally publishedYes

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Xanthine Oxidase
Lung Injury
Reperfusion
Ischemia
Bronchoalveolar Lavage
Liver
Reperfusion Injury
Alanine Transaminase
Thoracic Aorta
L-Lactate Dehydrogenase
Oxidants
Edema
Wounds and Injuries
Diet
Rabbits
Lung
Aorta
Proteins
tungstate
Enzymes

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Nielsen, V. G., Tan, S., Weinbroum, A., Mccammon, A. T., Samuelson, P. N., Gelman, S., & Parks, D. A. (1996). Lung injury after hepatoenteric ischemia-reperfusion: Role of xanthine oxidase. American Journal of Respiratory and Critical Care Medicine, 154(5), 1364-1369.

Lung injury after hepatoenteric ischemia-reperfusion : Role of xanthine oxidase. / Nielsen, Vance G.; Tan, Sidhartha; Weinbroum, Abraham; Mccammon, Andrew T.; Samuelson, Paul N.; Gelman, Simon; Parks, Dale A.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 154, No. 5, 1996, p. 1364-1369.

Research output: Contribution to journalArticle

Nielsen, VG, Tan, S, Weinbroum, A, Mccammon, AT, Samuelson, PN, Gelman, S & Parks, DA 1996, 'Lung injury after hepatoenteric ischemia-reperfusion: Role of xanthine oxidase', American Journal of Respiratory and Critical Care Medicine, vol. 154, no. 5, pp. 1364-1369.
Nielsen VG, Tan S, Weinbroum A, Mccammon AT, Samuelson PN, Gelman S et al. Lung injury after hepatoenteric ischemia-reperfusion: Role of xanthine oxidase. American Journal of Respiratory and Critical Care Medicine. 1996;154(5):1364-1369.
Nielsen, Vance G. ; Tan, Sidhartha ; Weinbroum, Abraham ; Mccammon, Andrew T. ; Samuelson, Paul N. ; Gelman, Simon ; Parks, Dale A. / Lung injury after hepatoenteric ischemia-reperfusion : Role of xanthine oxidase. In: American Journal of Respiratory and Critical Care Medicine. 1996 ; Vol. 154, No. 5. pp. 1364-1369.
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