Lymphatic muscle cells in rat mesenteric lymphatic vessels of various ages

Eric A. Bridenbaugh, Irina Tsoy Nizamutdinova, Daniel Jupiter, Takashi Nagai, Sangeetha Thangaswamy, Victor Chatterjee, Anatoliy A. Gashev

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Recent studies on aging-associated changes in mesenteric lymph flow in situ demonstrated predominance of the severe negative chronotropic effect of aging on the contractility of aged mesenteric lymphatic vessels (MLV). At the same time, contraction amplitude of the aged vessels was only slightly diminished by aging and can be rapidly stimulated within 5-15 minutes. However, the detailed quantitative evaluation of potential aging-associated changes in muscle cells investiture in MLV has never been performed. Methods and Results: In this study we, for the first time, performed detailed evaluation of muscle cells investiture in MLV in reference to the position of lymphatic valve in different zones of lymphangion within various age groups (3-mo, 9-mo and 24-mo Fischer-344 rats). Using visual and quantitative analyses of the images of MLV immunohistochemically labeled for actin, we confirmed that the zones located close upstream (pre-valve zones) and above lymphatic valves (valve zones) possess the lowest investiture of lymphatic muscle cells. Most of the high muscle cells investiture zones exist downstream to the lymphatic valve (post-valve zones). The muscle cells investiture of these zones is not affected by aging, while pre-valve and valve zones demonstrate significant aging-associated decrease in muscle cells investiture. Conclusions: The low muscle cells investiture zones in lymphatic vessels consist of predominantly longitudinally oriented muscle cells which are positioned in pre-valve and valve zones and connect adjacent lymphangions. These cells may provide important functional impact on the biomechanics of the lymphatic valve gating and electrical coupling between lymphangions, while their aging-associated changes may delimit adaptive reserves of aged lymphatic vessels.

Original languageEnglish (US)
Pages (from-to)35-42
Number of pages8
JournalLymphatic Research and Biology
Volume11
Issue number1
DOIs
StatePublished - Mar 1 2013
Externally publishedYes

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Lymphatic Vessels
Muscle Cells
Inbred F344 Rats
Lymph
Biomechanical Phenomena
Actins
Age Groups

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Bridenbaugh, E. A., Nizamutdinova, I. T., Jupiter, D., Nagai, T., Thangaswamy, S., Chatterjee, V., & Gashev, A. A. (2013). Lymphatic muscle cells in rat mesenteric lymphatic vessels of various ages. Lymphatic Research and Biology, 11(1), 35-42. https://doi.org/10.1089/lrb.2012.0025

Lymphatic muscle cells in rat mesenteric lymphatic vessels of various ages. / Bridenbaugh, Eric A.; Nizamutdinova, Irina Tsoy; Jupiter, Daniel; Nagai, Takashi; Thangaswamy, Sangeetha; Chatterjee, Victor; Gashev, Anatoliy A.

In: Lymphatic Research and Biology, Vol. 11, No. 1, 01.03.2013, p. 35-42.

Research output: Contribution to journalArticle

Bridenbaugh, EA, Nizamutdinova, IT, Jupiter, D, Nagai, T, Thangaswamy, S, Chatterjee, V & Gashev, AA 2013, 'Lymphatic muscle cells in rat mesenteric lymphatic vessels of various ages', Lymphatic Research and Biology, vol. 11, no. 1, pp. 35-42. https://doi.org/10.1089/lrb.2012.0025
Bridenbaugh EA, Nizamutdinova IT, Jupiter D, Nagai T, Thangaswamy S, Chatterjee V et al. Lymphatic muscle cells in rat mesenteric lymphatic vessels of various ages. Lymphatic Research and Biology. 2013 Mar 1;11(1):35-42. https://doi.org/10.1089/lrb.2012.0025
Bridenbaugh, Eric A. ; Nizamutdinova, Irina Tsoy ; Jupiter, Daniel ; Nagai, Takashi ; Thangaswamy, Sangeetha ; Chatterjee, Victor ; Gashev, Anatoliy A. / Lymphatic muscle cells in rat mesenteric lymphatic vessels of various ages. In: Lymphatic Research and Biology. 2013 ; Vol. 11, No. 1. pp. 35-42.
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