Lymphocyte autofluorescence

A screening procedure for neurodegenerative diseases

Edith P. Hawkins, Hal K. Hawkins, Dawna Armstrong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We examined the buffy coats from 12 consecutive patients with neurodegenerative diseases to determine the value of autofluorescence study by fluorescence microscopy in parallel with electron microscopy, as a rapid and inexpensive screening procedure for lymphocyte inclusions characteristic of neuronal ceroidlipofuscinoses. Four patients (3 with neuronal ceroidlipofuscinoses and 1 with Hallervorden-Spatz syndrome) had discrete, yellow-orange, 1-2 μm autofluorescent granules in the cytoplasm of some lymphocytes. These granules corresponded to characteristic inclusions of neuronal ceroidlipofuscinoses demonstrated by electron microscopy. Five patients (4 undiagnosed or nonspecific disease, and 1 with Leber congenital amaurosis) had hazy green cytoplasmic autofluorescence which correlated with parallel tubular arrays in lymphocytes. The 3 patients with no autofluorescence had no ultrastructural lymphocytic inclusions. We conclude that buffy coat autofluorescence is a rapid and inexpensive method of identifying specimens that require electron microscopic confirmation, and the absence of autofluorescence of lymphocytes in a buffy coat specimen eliminates the necessity for electron microscopic examination. However, characteristic ultrastructural inclusions associated with neurodegenerative diseases may occur in other tissues, such as skin and conjunctiva.

Original languageEnglish (US)
Pages (from-to)160-166
Number of pages7
JournalPediatric Neurology
Volume2
Issue number3
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Neurodegenerative Diseases
Lymphocytes
Electron Microscopy
Pantothenate Kinase-Associated Neurodegeneration
Electrons
Conjunctiva
Fluorescence Microscopy
Cytoplasm
Skin

ASJC Scopus subject areas

  • Clinical Neurology
  • Pediatrics, Perinatology, and Child Health
  • Developmental Neuroscience
  • Neurology

Cite this

Lymphocyte autofluorescence : A screening procedure for neurodegenerative diseases. / Hawkins, Edith P.; Hawkins, Hal K.; Armstrong, Dawna.

In: Pediatric Neurology, Vol. 2, No. 3, 1986, p. 160-166.

Research output: Contribution to journalArticle

Hawkins, Edith P. ; Hawkins, Hal K. ; Armstrong, Dawna. / Lymphocyte autofluorescence : A screening procedure for neurodegenerative diseases. In: Pediatric Neurology. 1986 ; Vol. 2, No. 3. pp. 160-166.
@article{c3e2b4dabe014d768293845b553fb40d,
title = "Lymphocyte autofluorescence: A screening procedure for neurodegenerative diseases",
abstract = "We examined the buffy coats from 12 consecutive patients with neurodegenerative diseases to determine the value of autofluorescence study by fluorescence microscopy in parallel with electron microscopy, as a rapid and inexpensive screening procedure for lymphocyte inclusions characteristic of neuronal ceroidlipofuscinoses. Four patients (3 with neuronal ceroidlipofuscinoses and 1 with Hallervorden-Spatz syndrome) had discrete, yellow-orange, 1-2 μm autofluorescent granules in the cytoplasm of some lymphocytes. These granules corresponded to characteristic inclusions of neuronal ceroidlipofuscinoses demonstrated by electron microscopy. Five patients (4 undiagnosed or nonspecific disease, and 1 with Leber congenital amaurosis) had hazy green cytoplasmic autofluorescence which correlated with parallel tubular arrays in lymphocytes. The 3 patients with no autofluorescence had no ultrastructural lymphocytic inclusions. We conclude that buffy coat autofluorescence is a rapid and inexpensive method of identifying specimens that require electron microscopic confirmation, and the absence of autofluorescence of lymphocytes in a buffy coat specimen eliminates the necessity for electron microscopic examination. However, characteristic ultrastructural inclusions associated with neurodegenerative diseases may occur in other tissues, such as skin and conjunctiva.",
author = "Hawkins, {Edith P.} and Hawkins, {Hal K.} and Dawna Armstrong",
year = "1986",
doi = "10.1016/0887-8994(86)90010-X",
language = "English (US)",
volume = "2",
pages = "160--166",
journal = "Pediatric Neurology",
issn = "0887-8994",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Lymphocyte autofluorescence

T2 - A screening procedure for neurodegenerative diseases

AU - Hawkins, Edith P.

AU - Hawkins, Hal K.

AU - Armstrong, Dawna

PY - 1986

Y1 - 1986

N2 - We examined the buffy coats from 12 consecutive patients with neurodegenerative diseases to determine the value of autofluorescence study by fluorescence microscopy in parallel with electron microscopy, as a rapid and inexpensive screening procedure for lymphocyte inclusions characteristic of neuronal ceroidlipofuscinoses. Four patients (3 with neuronal ceroidlipofuscinoses and 1 with Hallervorden-Spatz syndrome) had discrete, yellow-orange, 1-2 μm autofluorescent granules in the cytoplasm of some lymphocytes. These granules corresponded to characteristic inclusions of neuronal ceroidlipofuscinoses demonstrated by electron microscopy. Five patients (4 undiagnosed or nonspecific disease, and 1 with Leber congenital amaurosis) had hazy green cytoplasmic autofluorescence which correlated with parallel tubular arrays in lymphocytes. The 3 patients with no autofluorescence had no ultrastructural lymphocytic inclusions. We conclude that buffy coat autofluorescence is a rapid and inexpensive method of identifying specimens that require electron microscopic confirmation, and the absence of autofluorescence of lymphocytes in a buffy coat specimen eliminates the necessity for electron microscopic examination. However, characteristic ultrastructural inclusions associated with neurodegenerative diseases may occur in other tissues, such as skin and conjunctiva.

AB - We examined the buffy coats from 12 consecutive patients with neurodegenerative diseases to determine the value of autofluorescence study by fluorescence microscopy in parallel with electron microscopy, as a rapid and inexpensive screening procedure for lymphocyte inclusions characteristic of neuronal ceroidlipofuscinoses. Four patients (3 with neuronal ceroidlipofuscinoses and 1 with Hallervorden-Spatz syndrome) had discrete, yellow-orange, 1-2 μm autofluorescent granules in the cytoplasm of some lymphocytes. These granules corresponded to characteristic inclusions of neuronal ceroidlipofuscinoses demonstrated by electron microscopy. Five patients (4 undiagnosed or nonspecific disease, and 1 with Leber congenital amaurosis) had hazy green cytoplasmic autofluorescence which correlated with parallel tubular arrays in lymphocytes. The 3 patients with no autofluorescence had no ultrastructural lymphocytic inclusions. We conclude that buffy coat autofluorescence is a rapid and inexpensive method of identifying specimens that require electron microscopic confirmation, and the absence of autofluorescence of lymphocytes in a buffy coat specimen eliminates the necessity for electron microscopic examination. However, characteristic ultrastructural inclusions associated with neurodegenerative diseases may occur in other tissues, such as skin and conjunctiva.

UR - http://www.scopus.com/inward/record.url?scp=0022868272&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022868272&partnerID=8YFLogxK

U2 - 10.1016/0887-8994(86)90010-X

DO - 10.1016/0887-8994(86)90010-X

M3 - Article

VL - 2

SP - 160

EP - 166

JO - Pediatric Neurology

JF - Pediatric Neurology

SN - 0887-8994

IS - 3

ER -