M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections

Hideko Ohama, Akira Asai, Ichiaki Ito, Sumihiro Suzuki, Makiko Kobayashi, Kazuhide Higuchi, Fujio Suzuki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Alcohol abuse was found to predispose persons to opportunistic infections. In this study, we tried to improve the host antibacterial resistance of chronic alcohol-consuming (CAC) mice to opportunistic infections. Bactericidal macrophages with functions to produce IL-12 and to express mRNAs for CXCL9 and inducible nitric oxide synthase (M1 macrophages) were characterized as the main effector cells in host antibacterial innate immunities against infections with opportunistic pathogens. However, CAC mice were found to be carriers of M2b macrophages [macrophages with functions to produce IL-10 and to express mRNAs for CD163, chemokine ligand (CCL)1, and LIGHT (homologous to lymphotoxin, exhibits inducible expression, competes with herpes simplex virus glycoprotein D for high-voltage electron microscopy on T cells)], which were inhibitory on macrophage conversion from resident macrophages to M1 macrophages. Under treatment with CCL1 antisense oligodeoxynucleotides, a specific inhibitor of M2b macrophages, CAC mouse macrophages reverted to resident macrophages, and M1 macrophages were induced by a bacterial antigen from macrophages of CAC mice that were previously treated with the oligodeoxynucleotides. Opportunistic infections (enterococcal translocation and Klebsiella pneumonia) in CAC mice were completely controlled by CCL1 antisense oligodeoxynucleotides. These results indicate that certain opportunistic infections in alcoholics are controllable through the modulation of M2b macrophages.

Original languageEnglish (US)
Pages (from-to)420-431
Number of pages12
JournalAmerican Journal of Pathology
Volume185
Issue number2
DOIs
StatePublished - 2014

Fingerprint

Opportunistic Infections
Alcohol Drinking
Macrophages
Alcohols
Oligodeoxyribonucleotides
Bacterial Antigens
Lymphotoxin-alpha
Messenger RNA
Klebsiella pneumoniae
Nitric Oxide Synthase Type II
Simplexvirus
Alcoholics
Interleukin-12
Chemokines
Innate Immunity
Interleukin-10
Alcoholism
Electron Microscopy
Glycoproteins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections. / Ohama, Hideko; Asai, Akira; Ito, Ichiaki; Suzuki, Sumihiro; Kobayashi, Makiko; Higuchi, Kazuhide; Suzuki, Fujio.

In: American Journal of Pathology, Vol. 185, No. 2, 2014, p. 420-431.

Research output: Contribution to journalArticle

Ohama, Hideko ; Asai, Akira ; Ito, Ichiaki ; Suzuki, Sumihiro ; Kobayashi, Makiko ; Higuchi, Kazuhide ; Suzuki, Fujio. / M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections. In: American Journal of Pathology. 2014 ; Vol. 185, No. 2. pp. 420-431.
@article{b22d2fcf51834b569f03f13c9bd9ff52,
title = "M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections",
abstract = "Alcohol abuse was found to predispose persons to opportunistic infections. In this study, we tried to improve the host antibacterial resistance of chronic alcohol-consuming (CAC) mice to opportunistic infections. Bactericidal macrophages with functions to produce IL-12 and to express mRNAs for CXCL9 and inducible nitric oxide synthase (M1 macrophages) were characterized as the main effector cells in host antibacterial innate immunities against infections with opportunistic pathogens. However, CAC mice were found to be carriers of M2b macrophages [macrophages with functions to produce IL-10 and to express mRNAs for CD163, chemokine ligand (CCL)1, and LIGHT (homologous to lymphotoxin, exhibits inducible expression, competes with herpes simplex virus glycoprotein D for high-voltage electron microscopy on T cells)], which were inhibitory on macrophage conversion from resident macrophages to M1 macrophages. Under treatment with CCL1 antisense oligodeoxynucleotides, a specific inhibitor of M2b macrophages, CAC mouse macrophages reverted to resident macrophages, and M1 macrophages were induced by a bacterial antigen from macrophages of CAC mice that were previously treated with the oligodeoxynucleotides. Opportunistic infections (enterococcal translocation and Klebsiella pneumonia) in CAC mice were completely controlled by CCL1 antisense oligodeoxynucleotides. These results indicate that certain opportunistic infections in alcoholics are controllable through the modulation of M2b macrophages.",
author = "Hideko Ohama and Akira Asai and Ichiaki Ito and Sumihiro Suzuki and Makiko Kobayashi and Kazuhide Higuchi and Fujio Suzuki",
year = "2014",
doi = "10.1016/j.ajpath.2014.09.022",
language = "English (US)",
volume = "185",
pages = "420--431",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections

AU - Ohama, Hideko

AU - Asai, Akira

AU - Ito, Ichiaki

AU - Suzuki, Sumihiro

AU - Kobayashi, Makiko

AU - Higuchi, Kazuhide

AU - Suzuki, Fujio

PY - 2014

Y1 - 2014

N2 - Alcohol abuse was found to predispose persons to opportunistic infections. In this study, we tried to improve the host antibacterial resistance of chronic alcohol-consuming (CAC) mice to opportunistic infections. Bactericidal macrophages with functions to produce IL-12 and to express mRNAs for CXCL9 and inducible nitric oxide synthase (M1 macrophages) were characterized as the main effector cells in host antibacterial innate immunities against infections with opportunistic pathogens. However, CAC mice were found to be carriers of M2b macrophages [macrophages with functions to produce IL-10 and to express mRNAs for CD163, chemokine ligand (CCL)1, and LIGHT (homologous to lymphotoxin, exhibits inducible expression, competes with herpes simplex virus glycoprotein D for high-voltage electron microscopy on T cells)], which were inhibitory on macrophage conversion from resident macrophages to M1 macrophages. Under treatment with CCL1 antisense oligodeoxynucleotides, a specific inhibitor of M2b macrophages, CAC mouse macrophages reverted to resident macrophages, and M1 macrophages were induced by a bacterial antigen from macrophages of CAC mice that were previously treated with the oligodeoxynucleotides. Opportunistic infections (enterococcal translocation and Klebsiella pneumonia) in CAC mice were completely controlled by CCL1 antisense oligodeoxynucleotides. These results indicate that certain opportunistic infections in alcoholics are controllable through the modulation of M2b macrophages.

AB - Alcohol abuse was found to predispose persons to opportunistic infections. In this study, we tried to improve the host antibacterial resistance of chronic alcohol-consuming (CAC) mice to opportunistic infections. Bactericidal macrophages with functions to produce IL-12 and to express mRNAs for CXCL9 and inducible nitric oxide synthase (M1 macrophages) were characterized as the main effector cells in host antibacterial innate immunities against infections with opportunistic pathogens. However, CAC mice were found to be carriers of M2b macrophages [macrophages with functions to produce IL-10 and to express mRNAs for CD163, chemokine ligand (CCL)1, and LIGHT (homologous to lymphotoxin, exhibits inducible expression, competes with herpes simplex virus glycoprotein D for high-voltage electron microscopy on T cells)], which were inhibitory on macrophage conversion from resident macrophages to M1 macrophages. Under treatment with CCL1 antisense oligodeoxynucleotides, a specific inhibitor of M2b macrophages, CAC mouse macrophages reverted to resident macrophages, and M1 macrophages were induced by a bacterial antigen from macrophages of CAC mice that were previously treated with the oligodeoxynucleotides. Opportunistic infections (enterococcal translocation and Klebsiella pneumonia) in CAC mice were completely controlled by CCL1 antisense oligodeoxynucleotides. These results indicate that certain opportunistic infections in alcoholics are controllable through the modulation of M2b macrophages.

UR - http://www.scopus.com/inward/record.url?scp=84922309599&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922309599&partnerID=8YFLogxK

U2 - 10.1016/j.ajpath.2014.09.022

DO - 10.1016/j.ajpath.2014.09.022

M3 - Article

VL - 185

SP - 420

EP - 431

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 2

ER -