M2b macrophage polarization accompanied with reduction of long noncoding RNA GAS5

Ichiaki Ito, Akira Asai, Sumihiro Suzuki, Makiko Kobayashi, Fujio Suzuki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Macrophages (Mϕ(symbol)) are highly plastic and change their functional phenotypes depending on microenvironmental signals. Recent studies have shown that microRNAs are involved in the polarization of Mϕ(symbol). In this study, we demonstrated that the phenotype of M2bMϕ(symbol) [CCL1(+) IL-10(+) LIGHT(+)] switches to other phenotypes with interchangeability attained through the increased expression of growth arrest-specific 5 RNA (GAS5 RNA), a long noncoding RNA. GAS5 RNA has been described as a silencer of the CCL1 gene. Various phenotypes of Mϕ(symbol) were prepared from bone marrow-derived Mϕ(symbol) (BMDMϕ(symbol)) after stimulation with IFNγ [M(IFNγ)/M1Mϕ(symbol)], IL-4 [M(IL-4)/M2aMϕ(symbol)], LPS and immobilized IgG [M(LPS + IC)/M2bMϕ(symbol)], and IL-10 [M(IL-10)/M2cMϕ(symbol)]. BMDMϕ(symbol) cultured with medium [M(no)/quiescent Mϕ(symbol)] were used as a control. As compared to Μ(no), M(IFNγ), M(IL-4) and M(IL-10), the reduced level of GAS5 RNA was shown in M(LPS + IC). CCL1 and LIGHT mRNAs (typical biomarkers of M2bMϕ(symbol)) were not expressed by M(LPS + IC) transduced with a GAS5 gene using lentiviral vector. The reduction of GAS5 RNA in M(LPS + IC) was mediated by the activation of nonsense-mediated RNA decay (NMD) pathway. BMDMϕ(symbol) overexpressed with GAS5 RNA after GAS5 gene transduction did not polarize to M2bMϕ(symbol) even though they were stimulated with LPS and IC in combination. These results indicate that the reduction of GAS5 RNA influenced by the NMD pathway activation leads to the Mϕ(symbol) polarization stimulated with LPS and IC in combination.

Original languageEnglish (US)
JournalBiochemical and Biophysical Research Communications
DOIs
StateAccepted/In press - 2017

Fingerprint

Long Noncoding RNA
Macrophages
RNA
Polarization
Interleukin-10
Growth
Interleukin-4
Phenotype
Bone Marrow
RNA Stability
Bone
Genes
Light
Chemical activation
MicroRNAs
Plastics
Biomarkers
Immunoglobulin G
Messenger RNA
Switches

Keywords

  • Bone marrow-derived macrophages
  • Long noncoding RNA GAS5
  • Macrophage polarization
  • Nonsense-mediated RNA decay

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

M2b macrophage polarization accompanied with reduction of long noncoding RNA GAS5. / Ito, Ichiaki; Asai, Akira; Suzuki, Sumihiro; Kobayashi, Makiko; Suzuki, Fujio.

In: Biochemical and Biophysical Research Communications, 2017.

Research output: Contribution to journalArticle

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abstract = "Macrophages (Mϕ(symbol)) are highly plastic and change their functional phenotypes depending on microenvironmental signals. Recent studies have shown that microRNAs are involved in the polarization of Mϕ(symbol). In this study, we demonstrated that the phenotype of M2bMϕ(symbol) [CCL1(+) IL-10(+) LIGHT(+)] switches to other phenotypes with interchangeability attained through the increased expression of growth arrest-specific 5 RNA (GAS5 RNA), a long noncoding RNA. GAS5 RNA has been described as a silencer of the CCL1 gene. Various phenotypes of Mϕ(symbol) were prepared from bone marrow-derived Mϕ(symbol) (BMDMϕ(symbol)) after stimulation with IFNγ [M(IFNγ)/M1Mϕ(symbol)], IL-4 [M(IL-4)/M2aMϕ(symbol)], LPS and immobilized IgG [M(LPS + IC)/M2bMϕ(symbol)], and IL-10 [M(IL-10)/M2cMϕ(symbol)]. BMDMϕ(symbol) cultured with medium [M(no)/quiescent Mϕ(symbol)] were used as a control. As compared to Μ(no), M(IFNγ), M(IL-4) and M(IL-10), the reduced level of GAS5 RNA was shown in M(LPS + IC). CCL1 and LIGHT mRNAs (typical biomarkers of M2bMϕ(symbol)) were not expressed by M(LPS + IC) transduced with a GAS5 gene using lentiviral vector. The reduction of GAS5 RNA in M(LPS + IC) was mediated by the activation of nonsense-mediated RNA decay (NMD) pathway. BMDMϕ(symbol) overexpressed with GAS5 RNA after GAS5 gene transduction did not polarize to M2bMϕ(symbol) even though they were stimulated with LPS and IC in combination. These results indicate that the reduction of GAS5 RNA influenced by the NMD pathway activation leads to the Mϕ(symbol) polarization stimulated with LPS and IC in combination.",
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AU - Suzuki, Fujio

PY - 2017

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N2 - Macrophages (Mϕ(symbol)) are highly plastic and change their functional phenotypes depending on microenvironmental signals. Recent studies have shown that microRNAs are involved in the polarization of Mϕ(symbol). In this study, we demonstrated that the phenotype of M2bMϕ(symbol) [CCL1(+) IL-10(+) LIGHT(+)] switches to other phenotypes with interchangeability attained through the increased expression of growth arrest-specific 5 RNA (GAS5 RNA), a long noncoding RNA. GAS5 RNA has been described as a silencer of the CCL1 gene. Various phenotypes of Mϕ(symbol) were prepared from bone marrow-derived Mϕ(symbol) (BMDMϕ(symbol)) after stimulation with IFNγ [M(IFNγ)/M1Mϕ(symbol)], IL-4 [M(IL-4)/M2aMϕ(symbol)], LPS and immobilized IgG [M(LPS + IC)/M2bMϕ(symbol)], and IL-10 [M(IL-10)/M2cMϕ(symbol)]. BMDMϕ(symbol) cultured with medium [M(no)/quiescent Mϕ(symbol)] were used as a control. As compared to Μ(no), M(IFNγ), M(IL-4) and M(IL-10), the reduced level of GAS5 RNA was shown in M(LPS + IC). CCL1 and LIGHT mRNAs (typical biomarkers of M2bMϕ(symbol)) were not expressed by M(LPS + IC) transduced with a GAS5 gene using lentiviral vector. The reduction of GAS5 RNA in M(LPS + IC) was mediated by the activation of nonsense-mediated RNA decay (NMD) pathway. BMDMϕ(symbol) overexpressed with GAS5 RNA after GAS5 gene transduction did not polarize to M2bMϕ(symbol) even though they were stimulated with LPS and IC in combination. These results indicate that the reduction of GAS5 RNA influenced by the NMD pathway activation leads to the Mϕ(symbol) polarization stimulated with LPS and IC in combination.

AB - Macrophages (Mϕ(symbol)) are highly plastic and change their functional phenotypes depending on microenvironmental signals. Recent studies have shown that microRNAs are involved in the polarization of Mϕ(symbol). In this study, we demonstrated that the phenotype of M2bMϕ(symbol) [CCL1(+) IL-10(+) LIGHT(+)] switches to other phenotypes with interchangeability attained through the increased expression of growth arrest-specific 5 RNA (GAS5 RNA), a long noncoding RNA. GAS5 RNA has been described as a silencer of the CCL1 gene. Various phenotypes of Mϕ(symbol) were prepared from bone marrow-derived Mϕ(symbol) (BMDMϕ(symbol)) after stimulation with IFNγ [M(IFNγ)/M1Mϕ(symbol)], IL-4 [M(IL-4)/M2aMϕ(symbol)], LPS and immobilized IgG [M(LPS + IC)/M2bMϕ(symbol)], and IL-10 [M(IL-10)/M2cMϕ(symbol)]. BMDMϕ(symbol) cultured with medium [M(no)/quiescent Mϕ(symbol)] were used as a control. As compared to Μ(no), M(IFNγ), M(IL-4) and M(IL-10), the reduced level of GAS5 RNA was shown in M(LPS + IC). CCL1 and LIGHT mRNAs (typical biomarkers of M2bMϕ(symbol)) were not expressed by M(LPS + IC) transduced with a GAS5 gene using lentiviral vector. The reduction of GAS5 RNA in M(LPS + IC) was mediated by the activation of nonsense-mediated RNA decay (NMD) pathway. BMDMϕ(symbol) overexpressed with GAS5 RNA after GAS5 gene transduction did not polarize to M2bMϕ(symbol) even though they were stimulated with LPS and IC in combination. These results indicate that the reduction of GAS5 RNA influenced by the NMD pathway activation leads to the Mϕ(symbol) polarization stimulated with LPS and IC in combination.

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KW - Macrophage polarization

KW - Nonsense-mediated RNA decay

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