M2b monocytes predominated in peripheral blood of severely burned patients

Makiko Kobayashi, Marc G. Jeschke, Kenji Shigematsu, Akira Asai, Shohei Yoshida, David Herndon, Fujio Suzuki

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Severely burned patients were shown to be carriers of M2 monocytes, and all of the monocytes isolated from peripheral blood of severely burned patients (19 of 19 patients) were demonstrated as M2b monocytes (IL-12-IL-10 +CCL1+ monocytes). Low levels of M2a (IL-12 -IL-10+CCL17+ monocytes) and M2c monocytes (IL-12-IL-10+CXCL13+ monocytes) were demonstrated in peripheral blood of severely burned patients (M2a, 2 of 19 patients; M2c, 5 of 19 patients). M2b, M2a, and M2c monocytes were not detected in peripheral blood of healthy donors. However, M2b monocytes appeared when healthy donor monocytes were cultured in media supplemented with burn patient serum (15%). CCL2 was detected in sera of all burn patients, and M2b monocytes were not generated from healthy donor monocytes cultured with media containing 15% burn patient sera that were previously treated with anti-CCL2 mAb. In addition, M2b monocytes were generated from healthy donor monocytes in cultures supplemented with rCCL2. These results indicate that M2b monocytes are predominant in peripheral blood of severely burned patients who are carriers of CCL2 that functions to stimulate monocyte conversion from resident monocytes to M2b monocytes.

Original languageEnglish (US)
Pages (from-to)7174-7179
Number of pages6
JournalJournal of Immunology
Volume185
Issue number12
DOIs
StatePublished - Dec 15 2010

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Monocytes
Interleukin-12
Interleukin-10
Tissue Donors
Serum
Blood Donors

ASJC Scopus subject areas

  • Immunology

Cite this

Kobayashi, M., Jeschke, M. G., Shigematsu, K., Asai, A., Yoshida, S., Herndon, D., & Suzuki, F. (2010). M2b monocytes predominated in peripheral blood of severely burned patients. Journal of Immunology, 185(12), 7174-7179. https://doi.org/10.4049/jimmunol.0903935

M2b monocytes predominated in peripheral blood of severely burned patients. / Kobayashi, Makiko; Jeschke, Marc G.; Shigematsu, Kenji; Asai, Akira; Yoshida, Shohei; Herndon, David; Suzuki, Fujio.

In: Journal of Immunology, Vol. 185, No. 12, 15.12.2010, p. 7174-7179.

Research output: Contribution to journalArticle

Kobayashi, M, Jeschke, MG, Shigematsu, K, Asai, A, Yoshida, S, Herndon, D & Suzuki, F 2010, 'M2b monocytes predominated in peripheral blood of severely burned patients', Journal of Immunology, vol. 185, no. 12, pp. 7174-7179. https://doi.org/10.4049/jimmunol.0903935
Kobayashi M, Jeschke MG, Shigematsu K, Asai A, Yoshida S, Herndon D et al. M2b monocytes predominated in peripheral blood of severely burned patients. Journal of Immunology. 2010 Dec 15;185(12):7174-7179. https://doi.org/10.4049/jimmunol.0903935
Kobayashi, Makiko ; Jeschke, Marc G. ; Shigematsu, Kenji ; Asai, Akira ; Yoshida, Shohei ; Herndon, David ; Suzuki, Fujio. / M2b monocytes predominated in peripheral blood of severely burned patients. In: Journal of Immunology. 2010 ; Vol. 185, No. 12. pp. 7174-7179.
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