Macrophage Epithelial Reprogramming Underlies Mycobacterial Granuloma Formation and Promotes Infection

Mark R. Cronan, Rebecca W. Beerman, Allison F. Rosenberg, Joseph W. Saelens, Matthew G. Johnson, Stefan H. Oehlers, Dana M. Sisk, Kristen L. Jurcic Smith, Neil A. Medvitz, Sara E. Miller, Le A. Trinh, Scott E. Fraser, John F. Madden, Joanne Turner, Jason E. Stout, Sunhee Lee, David M. Tobin

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Mycobacterium tuberculosis infection in humans triggers formation of granulomas, which are tightly organized immune cell aggregates that are the central structure of tuberculosis. Infected and uninfected macrophages interdigitate, assuming an altered, flattened appearance. Although pathologists have described these changes for over a century, the molecular and cellular programs underlying this transition are unclear. Here, using the zebrafish-Mycobacterium marinum model, we found that mycobacterial granuloma formation is accompanied by macrophage induction of canonical epithelial molecules and structures. We identified fundamental macrophage reprogramming events that parallel E-cadherin-dependent mesenchymal-epithelial transitions. Macrophage-specific disruption of E-cadherin function resulted in disordered granuloma formation, enhanced immune cell access, decreased bacterial burden, and increased host survival, suggesting that the granuloma can also serve a bacteria-protective role. Granuloma macrophages in humans with tuberculosis were similarly transformed. Thus, during mycobacterial infection, granuloma macrophages are broadly reprogrammed by epithelial modules, and this reprogramming alters the trajectory of infection and the associated immune response.

Original languageEnglish (US)
Pages (from-to)861-876
Number of pages16
JournalImmunity
Volume45
Issue number4
DOIs
StatePublished - Oct 18 2016
Externally publishedYes

Fingerprint

Granuloma
Macrophages
Infection
Cadherins
Tuberculosis
Mycobacterium marinum
Mycobacterium Infections
Epithelial-Mesenchymal Transition
Zebrafish
Mycobacterium tuberculosis
Bacteria
Survival

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Cronan, M. R., Beerman, R. W., Rosenberg, A. F., Saelens, J. W., Johnson, M. G., Oehlers, S. H., ... Tobin, D. M. (2016). Macrophage Epithelial Reprogramming Underlies Mycobacterial Granuloma Formation and Promotes Infection. Immunity, 45(4), 861-876. https://doi.org/10.1016/j.immuni.2016.09.014

Macrophage Epithelial Reprogramming Underlies Mycobacterial Granuloma Formation and Promotes Infection. / Cronan, Mark R.; Beerman, Rebecca W.; Rosenberg, Allison F.; Saelens, Joseph W.; Johnson, Matthew G.; Oehlers, Stefan H.; Sisk, Dana M.; Jurcic Smith, Kristen L.; Medvitz, Neil A.; Miller, Sara E.; Trinh, Le A.; Fraser, Scott E.; Madden, John F.; Turner, Joanne; Stout, Jason E.; Lee, Sunhee; Tobin, David M.

In: Immunity, Vol. 45, No. 4, 18.10.2016, p. 861-876.

Research output: Contribution to journalArticle

Cronan, MR, Beerman, RW, Rosenberg, AF, Saelens, JW, Johnson, MG, Oehlers, SH, Sisk, DM, Jurcic Smith, KL, Medvitz, NA, Miller, SE, Trinh, LA, Fraser, SE, Madden, JF, Turner, J, Stout, JE, Lee, S & Tobin, DM 2016, 'Macrophage Epithelial Reprogramming Underlies Mycobacterial Granuloma Formation and Promotes Infection', Immunity, vol. 45, no. 4, pp. 861-876. https://doi.org/10.1016/j.immuni.2016.09.014
Cronan MR, Beerman RW, Rosenberg AF, Saelens JW, Johnson MG, Oehlers SH et al. Macrophage Epithelial Reprogramming Underlies Mycobacterial Granuloma Formation and Promotes Infection. Immunity. 2016 Oct 18;45(4):861-876. https://doi.org/10.1016/j.immuni.2016.09.014
Cronan, Mark R. ; Beerman, Rebecca W. ; Rosenberg, Allison F. ; Saelens, Joseph W. ; Johnson, Matthew G. ; Oehlers, Stefan H. ; Sisk, Dana M. ; Jurcic Smith, Kristen L. ; Medvitz, Neil A. ; Miller, Sara E. ; Trinh, Le A. ; Fraser, Scott E. ; Madden, John F. ; Turner, Joanne ; Stout, Jason E. ; Lee, Sunhee ; Tobin, David M. / Macrophage Epithelial Reprogramming Underlies Mycobacterial Granuloma Formation and Promotes Infection. In: Immunity. 2016 ; Vol. 45, No. 4. pp. 861-876.
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