Macrophage inflammatory protein-1α activates basophils and mast cells

Rafeul Alam, Patricia A. Forsythe, Susan Stafford, Michael A. Lett-Brown, J. Andrew Grant

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Macrophage inflammatory protein-1 (MIP) is a recently cloned cytokine that causes neutrophilic infiltration and induces an inflammatory response. We studied the effect of MIP-1α on histamine secretion from basophils and mast cells. Leukocytes from allergic and normal subjects were studied. MIP-1α caused dose-dependent release of histamine from basophils of 14 of 20 allergic donors at concentrations of 10-9-10-7 M, and the mean release was 13.50 ± 2.9% at the highest concentration. In the same experiments, the mean histamine release by anti-immunoglobulin E and monocyte chemotactic and activating factor (MCAF) (10-7 M) was 32 ± 7% and 31 ± 3%, respectively. The cells from only 2 of 10 normal subjects released histamine in response to MIP-1α. Histamine release by MIP-1α was rapid, and almost complete within the first 3 min. MIP-1α-induced degranulation was a calcium-dependent noncytotoxic process. MIP-1α showed chemotactic activity for purified basophils that was comparable to MCAF. Both MIP-1α and MCAF at 10-7 M concentration elicited a chemotactic response that was 40% of the maximal response to C5a (1 μg/ml). Murine MIP-1α induced histamine release from mouse peritoneal mast cells in a dose-dependent manner. Thus, we have established that MIP-1α is a novel activator of basophils and mast cells.

Original languageEnglish (US)
Pages (from-to)781-786
Number of pages6
JournalJournal of Experimental Medicine
Volume176
Issue number3
StatePublished - Sep 1 1992

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Macrophage Inflammatory Proteins
Basophils
Mast Cells
Histamine Release
Chemokine CCL2
Histamine
Immunoglobulin E
Leukocytes
Cytokines
Calcium

ASJC Scopus subject areas

  • Immunology

Cite this

Alam, R., Forsythe, P. A., Stafford, S., Lett-Brown, M. A., & Grant, J. A. (1992). Macrophage inflammatory protein-1α activates basophils and mast cells. Journal of Experimental Medicine, 176(3), 781-786.

Macrophage inflammatory protein-1α activates basophils and mast cells. / Alam, Rafeul; Forsythe, Patricia A.; Stafford, Susan; Lett-Brown, Michael A.; Grant, J. Andrew.

In: Journal of Experimental Medicine, Vol. 176, No. 3, 01.09.1992, p. 781-786.

Research output: Contribution to journalArticle

Alam, R, Forsythe, PA, Stafford, S, Lett-Brown, MA & Grant, JA 1992, 'Macrophage inflammatory protein-1α activates basophils and mast cells', Journal of Experimental Medicine, vol. 176, no. 3, pp. 781-786.
Alam R, Forsythe PA, Stafford S, Lett-Brown MA, Grant JA. Macrophage inflammatory protein-1α activates basophils and mast cells. Journal of Experimental Medicine. 1992 Sep 1;176(3):781-786.
Alam, Rafeul ; Forsythe, Patricia A. ; Stafford, Susan ; Lett-Brown, Michael A. ; Grant, J. Andrew. / Macrophage inflammatory protein-1α activates basophils and mast cells. In: Journal of Experimental Medicine. 1992 ; Vol. 176, No. 3. pp. 781-786.
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