Macrophage physiological function after superparamagnetic iron oxide labeling

Jong Kai Hsiao; Hung Hao Chu; Yu Hsiu Wang; Chih Wei Lai; Pi Tai Chou; Sung Tsang Hsieh; Jaw Lin Wang; Hon Man Liu

doi:10.1002/nbm.1260

Macrophage physiological function after superparamagnetic iron oxide labeling

Jong Kai Hsiao
, Hung Hao Chu
, Yu Hsiu Wang
, Chih Wei Lai
, Pi Tai Chou
, Sung Tsang Hsieh
, Jaw Lin Wang
, Hon Man Liu

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

Our goal was to analyze the changes in morphology and physiological function (phagocytosis, migratory capabilities, humoral and cellular response, and nitric oxide secretion) of murine macrophages after labeling with a clinically used superparamagnetic iron oxide (SPIO), ferucarbotran. In SPIO-treated macrophages, nanoparticles were taken up in the cytoplasm and accumulated in a membrane-bound organelle. Macrophage proliferation and viability were not modified after SPIO labeling. Phagocytic function decreased after labeling with only 10 μg Fe/mL. SPIO, whereas other functions including migration and production of tumor necrosis factor-α and nitric oxide increased at the highest SPIO concentration (100 μg Fe/mL).

Original language	English (US)
Pages (from-to)	820-829
Number of pages	10
Journal	NMR in Biomedicine
Volume	21
Issue number	8
DOIs	https://doi.org/10.1002/nbm.1260
State	Published - Oct 2008
Externally published	Yes

Keywords

Cytokine
Iron oxide
Migration
Phagocytosis
Proliferation
Rumor necrosis factor
Viability

ASJC Scopus subject areas

Molecular Medicine
Radiology Nuclear Medicine and imaging
Spectroscopy

Access to Document

10.1002/nbm.1260

Cite this

@article{a558831b9d0c42a5b70287e0ffe4bb8f,

title = "Macrophage physiological function after superparamagnetic iron oxide labeling",

abstract = "Our goal was to analyze the changes in morphology and physiological function (phagocytosis, migratory capabilities, humoral and cellular response, and nitric oxide secretion) of murine macrophages after labeling with a clinically used superparamagnetic iron oxide (SPIO), ferucarbotran. In SPIO-treated macrophages, nanoparticles were taken up in the cytoplasm and accumulated in a membrane-bound organelle. Macrophage proliferation and viability were not modified after SPIO labeling. Phagocytic function decreased after labeling with only 10 μg Fe/mL. SPIO, whereas other functions including migration and production of tumor necrosis factor-α and nitric oxide increased at the highest SPIO concentration (100 μg Fe/mL).",

keywords = "Cytokine, Iron oxide, Migration, Phagocytosis, Proliferation, Rumor necrosis factor, Viability",

author = "Hsiao, \{Jong Kai\} and Chu, \{Hung Hao\} and Wang, \{Yu Hsiu\} and Lai, \{Chih Wei\} and Chou, \{Pi Tai\} and Hsieh, \{Sung Tsang\} and Wang, \{Jaw Lin\} and Liu, \{Hon Man\}",

year = "2008",

month = oct,

doi = "10.1002/nbm.1260",

language = "English (US)",

volume = "21",

pages = "820--829",

journal = "NMR in Biomedicine",

issn = "0952-3480",

publisher = "John Wiley and Sons Ltd",

number = "8",

}

TY - JOUR

T1 - Macrophage physiological function after superparamagnetic iron oxide labeling

AU - Hsiao, Jong Kai

AU - Chu, Hung Hao

AU - Wang, Yu Hsiu

AU - Lai, Chih Wei

AU - Chou, Pi Tai

AU - Hsieh, Sung Tsang

AU - Wang, Jaw Lin

AU - Liu, Hon Man

PY - 2008/10

Y1 - 2008/10

N2 - Our goal was to analyze the changes in morphology and physiological function (phagocytosis, migratory capabilities, humoral and cellular response, and nitric oxide secretion) of murine macrophages after labeling with a clinically used superparamagnetic iron oxide (SPIO), ferucarbotran. In SPIO-treated macrophages, nanoparticles were taken up in the cytoplasm and accumulated in a membrane-bound organelle. Macrophage proliferation and viability were not modified after SPIO labeling. Phagocytic function decreased after labeling with only 10 μg Fe/mL. SPIO, whereas other functions including migration and production of tumor necrosis factor-α and nitric oxide increased at the highest SPIO concentration (100 μg Fe/mL).

AB - Our goal was to analyze the changes in morphology and physiological function (phagocytosis, migratory capabilities, humoral and cellular response, and nitric oxide secretion) of murine macrophages after labeling with a clinically used superparamagnetic iron oxide (SPIO), ferucarbotran. In SPIO-treated macrophages, nanoparticles were taken up in the cytoplasm and accumulated in a membrane-bound organelle. Macrophage proliferation and viability were not modified after SPIO labeling. Phagocytic function decreased after labeling with only 10 μg Fe/mL. SPIO, whereas other functions including migration and production of tumor necrosis factor-α and nitric oxide increased at the highest SPIO concentration (100 μg Fe/mL).

KW - Cytokine

KW - Iron oxide

KW - Migration

KW - Phagocytosis

KW - Proliferation

KW - Rumor necrosis factor

KW - Viability

UR - https://www.scopus.com/pages/publications/53849117141

UR - https://www.scopus.com/pages/publications/53849117141#tab=citedBy

U2 - 10.1002/nbm.1260

DO - 10.1002/nbm.1260

M3 - Article

C2 - 18470957

AN - SCOPUS:53849117141

SN - 0952-3480

VL - 21

SP - 820

EP - 829

JO - NMR in Biomedicine

JF - NMR in Biomedicine

IS - 8

ER -

Macrophage physiological function after superparamagnetic iron oxide labeling

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this