Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver

W. Samuel Fagg, Naiyou Liu, Ming Jim Yang, Ke Cheng, Eric Chung, Jae Sung Kim, Gordon Wu, Jeffrey Fair

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Attaining consistent robust engraftment in the structurally normal liver is an obstacle for cellular transplantation. Most experimental approaches to increase transplanted cells’ engraftment involve recipient-centered deleterious methods such as partial hepatectomy or irradiation which may be unsuitable in the clinic. Here, we present a cell-based strategy that increases engraftment into the structurally normal liver using a combination of magnetic targeting and proliferative endoderm progenitor (EPs) cells. Magnetic labeling has little effect on cell viability and differentiation, but in the presence of magnetic targeting, it increases the initial dwell time of transplanted EPs into the undamaged liver parenchyma. Consequently, greater cell retention in the liver is observed concomitantly with fewer transplanted cells in the lungs. These highly proliferative cells then significantly increase their biomass over time in the liver parenchyma, approaching nearly 4% of total liver cells 30 d after transplant. Therefore, the cell-based mechanisms of increased initial dwell time through magnetic targeting combined with high rate of proliferation in situ yield significant engraftment in the undamaged liver.

Original languageEnglish (US)
Pages (from-to)1868-1877
Number of pages10
JournalCell Transplantation
Volume26
Issue number12
DOIs
StatePublished - Dec 1 2017

Fingerprint

Stem cells
Liver
Stem Cells
Derivatives
Endoderm
Transplants
Hepatectomy
Biomass
Labeling
Cell Differentiation
Cell Survival
Transplantation
Cells
Irradiation
Lung

Keywords

  • engraftment
  • magnetic targeting
  • quiescent liver
  • stem cell

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this

Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver. / Fagg, W. Samuel; Liu, Naiyou; Yang, Ming Jim; Cheng, Ke; Chung, Eric; Kim, Jae Sung; Wu, Gordon; Fair, Jeffrey.

In: Cell Transplantation, Vol. 26, No. 12, 01.12.2017, p. 1868-1877.

Research output: Contribution to journalArticle

Fagg, W. Samuel ; Liu, Naiyou ; Yang, Ming Jim ; Cheng, Ke ; Chung, Eric ; Kim, Jae Sung ; Wu, Gordon ; Fair, Jeffrey. / Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver. In: Cell Transplantation. 2017 ; Vol. 26, No. 12. pp. 1868-1877.
@article{e8cc77c4ce774134b02a83770c60f25e,
title = "Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver",
abstract = "Attaining consistent robust engraftment in the structurally normal liver is an obstacle for cellular transplantation. Most experimental approaches to increase transplanted cells’ engraftment involve recipient-centered deleterious methods such as partial hepatectomy or irradiation which may be unsuitable in the clinic. Here, we present a cell-based strategy that increases engraftment into the structurally normal liver using a combination of magnetic targeting and proliferative endoderm progenitor (EPs) cells. Magnetic labeling has little effect on cell viability and differentiation, but in the presence of magnetic targeting, it increases the initial dwell time of transplanted EPs into the undamaged liver parenchyma. Consequently, greater cell retention in the liver is observed concomitantly with fewer transplanted cells in the lungs. These highly proliferative cells then significantly increase their biomass over time in the liver parenchyma, approaching nearly 4{\%} of total liver cells 30 d after transplant. Therefore, the cell-based mechanisms of increased initial dwell time through magnetic targeting combined with high rate of proliferation in situ yield significant engraftment in the undamaged liver.",
keywords = "engraftment, magnetic targeting, quiescent liver, stem cell",
author = "Fagg, {W. Samuel} and Naiyou Liu and Yang, {Ming Jim} and Ke Cheng and Eric Chung and Kim, {Jae Sung} and Gordon Wu and Jeffrey Fair",
year = "2017",
month = "12",
day = "1",
doi = "10.1177/0963689717737320",
language = "English (US)",
volume = "26",
pages = "1868--1877",
journal = "Cell Transplantation",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "12",

}

TY - JOUR

T1 - Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver

AU - Fagg, W. Samuel

AU - Liu, Naiyou

AU - Yang, Ming Jim

AU - Cheng, Ke

AU - Chung, Eric

AU - Kim, Jae Sung

AU - Wu, Gordon

AU - Fair, Jeffrey

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Attaining consistent robust engraftment in the structurally normal liver is an obstacle for cellular transplantation. Most experimental approaches to increase transplanted cells’ engraftment involve recipient-centered deleterious methods such as partial hepatectomy or irradiation which may be unsuitable in the clinic. Here, we present a cell-based strategy that increases engraftment into the structurally normal liver using a combination of magnetic targeting and proliferative endoderm progenitor (EPs) cells. Magnetic labeling has little effect on cell viability and differentiation, but in the presence of magnetic targeting, it increases the initial dwell time of transplanted EPs into the undamaged liver parenchyma. Consequently, greater cell retention in the liver is observed concomitantly with fewer transplanted cells in the lungs. These highly proliferative cells then significantly increase their biomass over time in the liver parenchyma, approaching nearly 4% of total liver cells 30 d after transplant. Therefore, the cell-based mechanisms of increased initial dwell time through magnetic targeting combined with high rate of proliferation in situ yield significant engraftment in the undamaged liver.

AB - Attaining consistent robust engraftment in the structurally normal liver is an obstacle for cellular transplantation. Most experimental approaches to increase transplanted cells’ engraftment involve recipient-centered deleterious methods such as partial hepatectomy or irradiation which may be unsuitable in the clinic. Here, we present a cell-based strategy that increases engraftment into the structurally normal liver using a combination of magnetic targeting and proliferative endoderm progenitor (EPs) cells. Magnetic labeling has little effect on cell viability and differentiation, but in the presence of magnetic targeting, it increases the initial dwell time of transplanted EPs into the undamaged liver parenchyma. Consequently, greater cell retention in the liver is observed concomitantly with fewer transplanted cells in the lungs. These highly proliferative cells then significantly increase their biomass over time in the liver parenchyma, approaching nearly 4% of total liver cells 30 d after transplant. Therefore, the cell-based mechanisms of increased initial dwell time through magnetic targeting combined with high rate of proliferation in situ yield significant engraftment in the undamaged liver.

KW - engraftment

KW - magnetic targeting

KW - quiescent liver

KW - stem cell

UR - http://www.scopus.com/inward/record.url?scp=85041747093&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041747093&partnerID=8YFLogxK

U2 - 10.1177/0963689717737320

DO - 10.1177/0963689717737320

M3 - Article

C2 - 29390880

AN - SCOPUS:85041747093

VL - 26

SP - 1868

EP - 1877

JO - Cell Transplantation

JF - Cell Transplantation

SN - 0963-6897

IS - 12

ER -