Male germ cell-specific protein Trs4 binds to multiple proteins

Yu Qiang Shi, Yin Chuan Li, Xiao Qian Hu, Tao Liu, Shang Ying Liao, Jian Guo, Lin Huang, Zhao Yuan Hu, Alan Y B Tang, Kai Fai Lee, William S B Yeung, Chun Sheng Han, Yi Xun Liu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Temperature-related sequence 4 (Trs4) has been identified as a testis-specific gene with expression sensitive to the abdominal temperature changes induced by artificial cryptorchidism. In murine testes, Trs4 mRNA was detected in round spermatids and its protein was localized mainly in the elongating spermatids as well as in the acrosomes and tails of mature spermatozoa. Using a yeast two-hybrid screening system, we identified Rshl-2, Gstmu1, and Ddc8 as putative binding partners of the Trs4 protein in mouse testes. Their interactions were confirmed by in vivo and in vitro binding assays. Further studies demonstrated that Ddc8, a newly identified gene with unknown functions, displayed a similar expression pattern with Trs4 in mouse testes. In particular, Trs4, Ddc8, and Rshl-2 proteins were co-localized to the tails of mature spermatozoa. These results suggested that Trs4 might be involved in diverse processes of spermiogenesis and/or fertilization through interactions with its multiple binding partners.

Original languageEnglish (US)
Pages (from-to)583-588
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume388
Issue number3
DOIs
StatePublished - Oct 23 2009
Externally publishedYes

Fingerprint

Germ Cells
Cells
Temperature
Testis
Proteins
Spermatids
Spermatozoa
Tail
Genes
Acrosome
Two-Hybrid System Techniques
Cryptorchidism
Sexual Partners
Spermatogenesis
Fertilization
Yeast
Assays
Screening
Gene Expression
Messenger RNA

Keywords

  • Cryptorchidism
  • IQ calmodulin-binding motif
  • Spermatogenesis
  • Testis
  • Trs4

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Shi, Y. Q., Li, Y. C., Hu, X. Q., Liu, T., Liao, S. Y., Guo, J., ... Liu, Y. X. (2009). Male germ cell-specific protein Trs4 binds to multiple proteins. Biochemical and Biophysical Research Communications, 388(3), 583-588. https://doi.org/10.1016/j.bbrc.2009.08.053

Male germ cell-specific protein Trs4 binds to multiple proteins. / Shi, Yu Qiang; Li, Yin Chuan; Hu, Xiao Qian; Liu, Tao; Liao, Shang Ying; Guo, Jian; Huang, Lin; Hu, Zhao Yuan; Tang, Alan Y B; Lee, Kai Fai; Yeung, William S B; Han, Chun Sheng; Liu, Yi Xun.

In: Biochemical and Biophysical Research Communications, Vol. 388, No. 3, 23.10.2009, p. 583-588.

Research output: Contribution to journalArticle

Shi, YQ, Li, YC, Hu, XQ, Liu, T, Liao, SY, Guo, J, Huang, L, Hu, ZY, Tang, AYB, Lee, KF, Yeung, WSB, Han, CS & Liu, YX 2009, 'Male germ cell-specific protein Trs4 binds to multiple proteins', Biochemical and Biophysical Research Communications, vol. 388, no. 3, pp. 583-588. https://doi.org/10.1016/j.bbrc.2009.08.053
Shi, Yu Qiang ; Li, Yin Chuan ; Hu, Xiao Qian ; Liu, Tao ; Liao, Shang Ying ; Guo, Jian ; Huang, Lin ; Hu, Zhao Yuan ; Tang, Alan Y B ; Lee, Kai Fai ; Yeung, William S B ; Han, Chun Sheng ; Liu, Yi Xun. / Male germ cell-specific protein Trs4 binds to multiple proteins. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 388, No. 3. pp. 583-588.
@article{701ae9de99734290aba63849352f0cd7,
title = "Male germ cell-specific protein Trs4 binds to multiple proteins",
abstract = "Temperature-related sequence 4 (Trs4) has been identified as a testis-specific gene with expression sensitive to the abdominal temperature changes induced by artificial cryptorchidism. In murine testes, Trs4 mRNA was detected in round spermatids and its protein was localized mainly in the elongating spermatids as well as in the acrosomes and tails of mature spermatozoa. Using a yeast two-hybrid screening system, we identified Rshl-2, Gstmu1, and Ddc8 as putative binding partners of the Trs4 protein in mouse testes. Their interactions were confirmed by in vivo and in vitro binding assays. Further studies demonstrated that Ddc8, a newly identified gene with unknown functions, displayed a similar expression pattern with Trs4 in mouse testes. In particular, Trs4, Ddc8, and Rshl-2 proteins were co-localized to the tails of mature spermatozoa. These results suggested that Trs4 might be involved in diverse processes of spermiogenesis and/or fertilization through interactions with its multiple binding partners.",
keywords = "Cryptorchidism, IQ calmodulin-binding motif, Spermatogenesis, Testis, Trs4",
author = "Shi, {Yu Qiang} and Li, {Yin Chuan} and Hu, {Xiao Qian} and Tao Liu and Liao, {Shang Ying} and Jian Guo and Lin Huang and Hu, {Zhao Yuan} and Tang, {Alan Y B} and Lee, {Kai Fai} and Yeung, {William S B} and Han, {Chun Sheng} and Liu, {Yi Xun}",
year = "2009",
month = "10",
day = "23",
doi = "10.1016/j.bbrc.2009.08.053",
language = "English (US)",
volume = "388",
pages = "583--588",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Male germ cell-specific protein Trs4 binds to multiple proteins

AU - Shi, Yu Qiang

AU - Li, Yin Chuan

AU - Hu, Xiao Qian

AU - Liu, Tao

AU - Liao, Shang Ying

AU - Guo, Jian

AU - Huang, Lin

AU - Hu, Zhao Yuan

AU - Tang, Alan Y B

AU - Lee, Kai Fai

AU - Yeung, William S B

AU - Han, Chun Sheng

AU - Liu, Yi Xun

PY - 2009/10/23

Y1 - 2009/10/23

N2 - Temperature-related sequence 4 (Trs4) has been identified as a testis-specific gene with expression sensitive to the abdominal temperature changes induced by artificial cryptorchidism. In murine testes, Trs4 mRNA was detected in round spermatids and its protein was localized mainly in the elongating spermatids as well as in the acrosomes and tails of mature spermatozoa. Using a yeast two-hybrid screening system, we identified Rshl-2, Gstmu1, and Ddc8 as putative binding partners of the Trs4 protein in mouse testes. Their interactions were confirmed by in vivo and in vitro binding assays. Further studies demonstrated that Ddc8, a newly identified gene with unknown functions, displayed a similar expression pattern with Trs4 in mouse testes. In particular, Trs4, Ddc8, and Rshl-2 proteins were co-localized to the tails of mature spermatozoa. These results suggested that Trs4 might be involved in diverse processes of spermiogenesis and/or fertilization through interactions with its multiple binding partners.

AB - Temperature-related sequence 4 (Trs4) has been identified as a testis-specific gene with expression sensitive to the abdominal temperature changes induced by artificial cryptorchidism. In murine testes, Trs4 mRNA was detected in round spermatids and its protein was localized mainly in the elongating spermatids as well as in the acrosomes and tails of mature spermatozoa. Using a yeast two-hybrid screening system, we identified Rshl-2, Gstmu1, and Ddc8 as putative binding partners of the Trs4 protein in mouse testes. Their interactions were confirmed by in vivo and in vitro binding assays. Further studies demonstrated that Ddc8, a newly identified gene with unknown functions, displayed a similar expression pattern with Trs4 in mouse testes. In particular, Trs4, Ddc8, and Rshl-2 proteins were co-localized to the tails of mature spermatozoa. These results suggested that Trs4 might be involved in diverse processes of spermiogenesis and/or fertilization through interactions with its multiple binding partners.

KW - Cryptorchidism

KW - IQ calmodulin-binding motif

KW - Spermatogenesis

KW - Testis

KW - Trs4

UR - http://www.scopus.com/inward/record.url?scp=69549138575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69549138575&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.08.053

DO - 10.1016/j.bbrc.2009.08.053

M3 - Article

VL - 388

SP - 583

EP - 588

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -