Malignant melanoma induces migration and invasion of adult mesenchymal stem cells

Tammara Watts, Ruwen Cui

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives/Hypothesis: To determine if melanoma cells secrete chemotactic factors that result in the migration of multipotent stem cells. Study Design: In vitro cell culture. Methods: Chemotaxis and invasion of human mesenchymal stem cells (hMSCs) was determined using the modified Boyden chamber assay. Quantification of growth factors secreted by melanoma cells (A375) was determined using enzyme-linked immunosorbent assay. Results: Conditioned A375 melanoma media caused significant migration and invasion of hMSCs compared to serum-free controls and conditioned media from normal melanocytes (P <.0001). The migratory effect appeared maximal after the A375 media was conditioned for 48 hours. Physiologically relevant concentrations of fibroblast growth factor-2 (FGF2) (90 pg/mL) secreted by A375 melanoma cells caused significant migration of hMSCs (P <.001) compared to serum-free and normal melanocyte controls. Neutralization of FGF2 inhibited the migration of hMSCs to that of the negative controls (conditioned media from normal melanocytes). Conclusions: The melanoma tumor microenvironment may be maintained through chemotaxis and invasion of multipotent hMSCs, and this migratory effect appears to be mediated in part through secretion of FGF2 by melanoma cells.

Original languageEnglish (US)
Pages (from-to)2769-2772
Number of pages4
JournalLaryngoscope
Volume122
Issue number12
DOIs
StatePublished - Dec 2012

Fingerprint

Adult Stem Cells
Mesenchymal Stromal Cells
Melanoma
Melanocytes
Fibroblast Growth Factor 2
Conditioned Culture Medium
Chemotaxis
Multipotent Stem Cells
Tumor Microenvironment
Chemotactic Factors
Serum-Free Culture Media
Intercellular Signaling Peptides and Proteins
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay
Serum

Keywords

  • FGF2
  • Head and neck cancer
  • mesenchymal stem cells
  • migration

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Malignant melanoma induces migration and invasion of adult mesenchymal stem cells. / Watts, Tammara; Cui, Ruwen.

In: Laryngoscope, Vol. 122, No. 12, 12.2012, p. 2769-2772.

Research output: Contribution to journalArticle

Watts, Tammara ; Cui, Ruwen. / Malignant melanoma induces migration and invasion of adult mesenchymal stem cells. In: Laryngoscope. 2012 ; Vol. 122, No. 12. pp. 2769-2772.
@article{250e8d5c1af84e30b61910b210135258,
title = "Malignant melanoma induces migration and invasion of adult mesenchymal stem cells",
abstract = "Objectives/Hypothesis: To determine if melanoma cells secrete chemotactic factors that result in the migration of multipotent stem cells. Study Design: In vitro cell culture. Methods: Chemotaxis and invasion of human mesenchymal stem cells (hMSCs) was determined using the modified Boyden chamber assay. Quantification of growth factors secreted by melanoma cells (A375) was determined using enzyme-linked immunosorbent assay. Results: Conditioned A375 melanoma media caused significant migration and invasion of hMSCs compared to serum-free controls and conditioned media from normal melanocytes (P <.0001). The migratory effect appeared maximal after the A375 media was conditioned for 48 hours. Physiologically relevant concentrations of fibroblast growth factor-2 (FGF2) (90 pg/mL) secreted by A375 melanoma cells caused significant migration of hMSCs (P <.001) compared to serum-free and normal melanocyte controls. Neutralization of FGF2 inhibited the migration of hMSCs to that of the negative controls (conditioned media from normal melanocytes). Conclusions: The melanoma tumor microenvironment may be maintained through chemotaxis and invasion of multipotent hMSCs, and this migratory effect appears to be mediated in part through secretion of FGF2 by melanoma cells.",
keywords = "FGF2, Head and neck cancer, mesenchymal stem cells, migration",
author = "Tammara Watts and Ruwen Cui",
year = "2012",
month = "12",
doi = "10.1002/lary.23652",
language = "English (US)",
volume = "122",
pages = "2769--2772",
journal = "Laryngoscope",
issn = "0023-852X",
publisher = "John Wiley and Sons Inc.",
number = "12",

}

TY - JOUR

T1 - Malignant melanoma induces migration and invasion of adult mesenchymal stem cells

AU - Watts, Tammara

AU - Cui, Ruwen

PY - 2012/12

Y1 - 2012/12

N2 - Objectives/Hypothesis: To determine if melanoma cells secrete chemotactic factors that result in the migration of multipotent stem cells. Study Design: In vitro cell culture. Methods: Chemotaxis and invasion of human mesenchymal stem cells (hMSCs) was determined using the modified Boyden chamber assay. Quantification of growth factors secreted by melanoma cells (A375) was determined using enzyme-linked immunosorbent assay. Results: Conditioned A375 melanoma media caused significant migration and invasion of hMSCs compared to serum-free controls and conditioned media from normal melanocytes (P <.0001). The migratory effect appeared maximal after the A375 media was conditioned for 48 hours. Physiologically relevant concentrations of fibroblast growth factor-2 (FGF2) (90 pg/mL) secreted by A375 melanoma cells caused significant migration of hMSCs (P <.001) compared to serum-free and normal melanocyte controls. Neutralization of FGF2 inhibited the migration of hMSCs to that of the negative controls (conditioned media from normal melanocytes). Conclusions: The melanoma tumor microenvironment may be maintained through chemotaxis and invasion of multipotent hMSCs, and this migratory effect appears to be mediated in part through secretion of FGF2 by melanoma cells.

AB - Objectives/Hypothesis: To determine if melanoma cells secrete chemotactic factors that result in the migration of multipotent stem cells. Study Design: In vitro cell culture. Methods: Chemotaxis and invasion of human mesenchymal stem cells (hMSCs) was determined using the modified Boyden chamber assay. Quantification of growth factors secreted by melanoma cells (A375) was determined using enzyme-linked immunosorbent assay. Results: Conditioned A375 melanoma media caused significant migration and invasion of hMSCs compared to serum-free controls and conditioned media from normal melanocytes (P <.0001). The migratory effect appeared maximal after the A375 media was conditioned for 48 hours. Physiologically relevant concentrations of fibroblast growth factor-2 (FGF2) (90 pg/mL) secreted by A375 melanoma cells caused significant migration of hMSCs (P <.001) compared to serum-free and normal melanocyte controls. Neutralization of FGF2 inhibited the migration of hMSCs to that of the negative controls (conditioned media from normal melanocytes). Conclusions: The melanoma tumor microenvironment may be maintained through chemotaxis and invasion of multipotent hMSCs, and this migratory effect appears to be mediated in part through secretion of FGF2 by melanoma cells.

KW - FGF2

KW - Head and neck cancer

KW - mesenchymal stem cells

KW - migration

UR - http://www.scopus.com/inward/record.url?scp=84871275705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871275705&partnerID=8YFLogxK

U2 - 10.1002/lary.23652

DO - 10.1002/lary.23652

M3 - Article

VL - 122

SP - 2769

EP - 2772

JO - Laryngoscope

JF - Laryngoscope

SN - 0023-852X

IS - 12

ER -