Malnutrition alters the innate immune response and increases early visceralization following Leishmania donovani infection

G. M. Anstead, B. Chandrasekar, W. Zhao, J. Yang, L. E. Perez, Peter Melby

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Malnutrition is a risk factor for the development of visceral leishmaniasis. However, the immunological basis for this susceptibility is unknown. We have developed a mouse model to study the effect of malnutrition on innate immunity and early visceralization following Leishmania donovani infection. Three deficient diets were studied, including 6, 3, or 1% protein; these diets were also deficient in iron, zinc, and calories. The control diet contained 17% protein, was zinc and iron sufficient, and was provided ab libitum. Three days after infection with L. donovani promastigotes, the total extradermal (lymph nodes, liver, and spleen) and skin parasite burdens were equivalent in the malnourished (3% protein) and control mice, but in the malnourished group, a greater percentage (39.8 and 4.0%, respectively; P = 0.009) of the extradermal parasite burden was contained in the spleen and liver. The comparable levels of parasites in the footpads in the two diet groups and the higher lymph node parasite burdens in the well-nourished mice indicated that the higher visceral parasite burdens in the malnourished mice were not due to a deficit in local parasite killing but to a failure of lymph node barrier function. Lymph node cells from the malnourished, infected mice produced increased levels of prostaglandin E2 (PGE2) and decreased levels of interleukin-10. Inducible nitric oxide synthase activity was significantly lower in the spleen and liver of the malnourished mice. Thus, malnutrition causes a failure of lymph node barrier function after L. donovani infection, which may be related to excessive production of PGE2 and decreased levels of IL-10 and nitric oxide.

Original languageEnglish (US)
Pages (from-to)4709-4718
Number of pages10
JournalInfection and Immunity
Volume69
Issue number8
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Leishmania donovani
Innate Immunity
Malnutrition
Parasites
Lymph Nodes
Infection
Diet
Spleen
Dinoprostone
Interleukin-10
Zinc
Liver
Iron
Proteins
Visceral Leishmaniasis
Nitric Oxide Synthase Type II
Nitric Oxide
Skin

ASJC Scopus subject areas

  • Immunology

Cite this

Malnutrition alters the innate immune response and increases early visceralization following Leishmania donovani infection. / Anstead, G. M.; Chandrasekar, B.; Zhao, W.; Yang, J.; Perez, L. E.; Melby, Peter.

In: Infection and Immunity, Vol. 69, No. 8, 2001, p. 4709-4718.

Research output: Contribution to journalArticle

Anstead, G. M. ; Chandrasekar, B. ; Zhao, W. ; Yang, J. ; Perez, L. E. ; Melby, Peter. / Malnutrition alters the innate immune response and increases early visceralization following Leishmania donovani infection. In: Infection and Immunity. 2001 ; Vol. 69, No. 8. pp. 4709-4718.
@article{fb998a38cd5c4de4878b38ef087031d8,
title = "Malnutrition alters the innate immune response and increases early visceralization following Leishmania donovani infection",
abstract = "Malnutrition is a risk factor for the development of visceral leishmaniasis. However, the immunological basis for this susceptibility is unknown. We have developed a mouse model to study the effect of malnutrition on innate immunity and early visceralization following Leishmania donovani infection. Three deficient diets were studied, including 6, 3, or 1{\%} protein; these diets were also deficient in iron, zinc, and calories. The control diet contained 17{\%} protein, was zinc and iron sufficient, and was provided ab libitum. Three days after infection with L. donovani promastigotes, the total extradermal (lymph nodes, liver, and spleen) and skin parasite burdens were equivalent in the malnourished (3{\%} protein) and control mice, but in the malnourished group, a greater percentage (39.8 and 4.0{\%}, respectively; P = 0.009) of the extradermal parasite burden was contained in the spleen and liver. The comparable levels of parasites in the footpads in the two diet groups and the higher lymph node parasite burdens in the well-nourished mice indicated that the higher visceral parasite burdens in the malnourished mice were not due to a deficit in local parasite killing but to a failure of lymph node barrier function. Lymph node cells from the malnourished, infected mice produced increased levels of prostaglandin E2 (PGE2) and decreased levels of interleukin-10. Inducible nitric oxide synthase activity was significantly lower in the spleen and liver of the malnourished mice. Thus, malnutrition causes a failure of lymph node barrier function after L. donovani infection, which may be related to excessive production of PGE2 and decreased levels of IL-10 and nitric oxide.",
author = "Anstead, {G. M.} and B. Chandrasekar and W. Zhao and J. Yang and Perez, {L. E.} and Peter Melby",
year = "2001",
doi = "10.1128/IAI.69.8.4709-4718.2001",
language = "English (US)",
volume = "69",
pages = "4709--4718",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - Malnutrition alters the innate immune response and increases early visceralization following Leishmania donovani infection

AU - Anstead, G. M.

AU - Chandrasekar, B.

AU - Zhao, W.

AU - Yang, J.

AU - Perez, L. E.

AU - Melby, Peter

PY - 2001

Y1 - 2001

N2 - Malnutrition is a risk factor for the development of visceral leishmaniasis. However, the immunological basis for this susceptibility is unknown. We have developed a mouse model to study the effect of malnutrition on innate immunity and early visceralization following Leishmania donovani infection. Three deficient diets were studied, including 6, 3, or 1% protein; these diets were also deficient in iron, zinc, and calories. The control diet contained 17% protein, was zinc and iron sufficient, and was provided ab libitum. Three days after infection with L. donovani promastigotes, the total extradermal (lymph nodes, liver, and spleen) and skin parasite burdens were equivalent in the malnourished (3% protein) and control mice, but in the malnourished group, a greater percentage (39.8 and 4.0%, respectively; P = 0.009) of the extradermal parasite burden was contained in the spleen and liver. The comparable levels of parasites in the footpads in the two diet groups and the higher lymph node parasite burdens in the well-nourished mice indicated that the higher visceral parasite burdens in the malnourished mice were not due to a deficit in local parasite killing but to a failure of lymph node barrier function. Lymph node cells from the malnourished, infected mice produced increased levels of prostaglandin E2 (PGE2) and decreased levels of interleukin-10. Inducible nitric oxide synthase activity was significantly lower in the spleen and liver of the malnourished mice. Thus, malnutrition causes a failure of lymph node barrier function after L. donovani infection, which may be related to excessive production of PGE2 and decreased levels of IL-10 and nitric oxide.

AB - Malnutrition is a risk factor for the development of visceral leishmaniasis. However, the immunological basis for this susceptibility is unknown. We have developed a mouse model to study the effect of malnutrition on innate immunity and early visceralization following Leishmania donovani infection. Three deficient diets were studied, including 6, 3, or 1% protein; these diets were also deficient in iron, zinc, and calories. The control diet contained 17% protein, was zinc and iron sufficient, and was provided ab libitum. Three days after infection with L. donovani promastigotes, the total extradermal (lymph nodes, liver, and spleen) and skin parasite burdens were equivalent in the malnourished (3% protein) and control mice, but in the malnourished group, a greater percentage (39.8 and 4.0%, respectively; P = 0.009) of the extradermal parasite burden was contained in the spleen and liver. The comparable levels of parasites in the footpads in the two diet groups and the higher lymph node parasite burdens in the well-nourished mice indicated that the higher visceral parasite burdens in the malnourished mice were not due to a deficit in local parasite killing but to a failure of lymph node barrier function. Lymph node cells from the malnourished, infected mice produced increased levels of prostaglandin E2 (PGE2) and decreased levels of interleukin-10. Inducible nitric oxide synthase activity was significantly lower in the spleen and liver of the malnourished mice. Thus, malnutrition causes a failure of lymph node barrier function after L. donovani infection, which may be related to excessive production of PGE2 and decreased levels of IL-10 and nitric oxide.

UR - http://www.scopus.com/inward/record.url?scp=0034923317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034923317&partnerID=8YFLogxK

U2 - 10.1128/IAI.69.8.4709-4718.2001

DO - 10.1128/IAI.69.8.4709-4718.2001

M3 - Article

VL - 69

SP - 4709

EP - 4718

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 8

ER -