Malpais spring virus is a new species in the genus vesiculovirus

Nikos Vasilakis, Steven Widen, Amelia P A Travassos Da Rosa, Thomas Wood, Peter J. Walker, Edward C. Holmes, Robert B. Tesh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Malpais Spring virus (MSPV) is a mosquito-borne rhabdovirus that infects a variety of wild and feral ungulates in New Mexico, including horses and deer. Although, initial serologic tests and electron microscopy at the time of isolation nearly 25 years ago provided evidence that MSPV is a novel virus, possibly related to vesiculoviruses, the virus still has not been approved as a new species. Findings: Use of the illumina platform allowed us to obtain the complete genome of MSPV. Analysis of the complete 11019 nt genome sequence of the prototype 85-488NM strain of MSPV indicates that it encodes the five common rhabdovirus structural proteins (N, P, M, G and L) with alternative ORFs (> 180 nt) in the N, M and G genes, including a 249 nt ORF in the G gene predicted to encode a 9.26 kDa highly basic transmembrane protein. Although antigenically very distant, phylogenetic analysis of the L gene indicates that MSPV is most closely related to Jurona virus, also isolated from mosquitoes in Brazil, as well as a number of other vesiculoviruses. Conclusions: In sum, our analysis indicates MSPV should be classified as a member of the genus Vesiculovirus, family Rhabdoviridae. The complete genome sequence of MSPV will be helpful in the development of a reverse genetics system to study the unique aspects of this vesiculovirus in vivo and in vitro, and will assist development of specific diagnostic tests to study the epidemiology of MSPV infection.

Original languageEnglish (US)
Article number69
JournalVirology Journal
Volume10
DOIs
StatePublished - 2014

Fingerprint

Vesiculovirus
Viruses
Rhabdoviridae
Genome
Culicidae
Open Reading Frames
Genes
Reverse Genetics
Deer
Serologic Tests
Virus Diseases
Routine Diagnostic Tests
Horses
Brazil

Keywords

  • Family rhabdoviridae
  • Genome sequence
  • Genus vesiculovirus
  • Malpais spring virus (MSPV)
  • Phylogeny

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Vasilakis, N., Widen, S., Travassos Da Rosa, A. P. A., Wood, T., Walker, P. J., Holmes, E. C., & Tesh, R. B. (2014). Malpais spring virus is a new species in the genus vesiculovirus. Virology Journal, 10, [69]. https://doi.org/10.1186/1743-422X-10-69

Malpais spring virus is a new species in the genus vesiculovirus. / Vasilakis, Nikos; Widen, Steven; Travassos Da Rosa, Amelia P A; Wood, Thomas; Walker, Peter J.; Holmes, Edward C.; Tesh, Robert B.

In: Virology Journal, Vol. 10, 69, 2014.

Research output: Contribution to journalArticle

Vasilakis, N, Widen, S, Travassos Da Rosa, APA, Wood, T, Walker, PJ, Holmes, EC & Tesh, RB 2014, 'Malpais spring virus is a new species in the genus vesiculovirus', Virology Journal, vol. 10, 69. https://doi.org/10.1186/1743-422X-10-69
Vasilakis, Nikos ; Widen, Steven ; Travassos Da Rosa, Amelia P A ; Wood, Thomas ; Walker, Peter J. ; Holmes, Edward C. ; Tesh, Robert B. / Malpais spring virus is a new species in the genus vesiculovirus. In: Virology Journal. 2014 ; Vol. 10.
@article{2b853fb92697446abe5369616dde7945,
title = "Malpais spring virus is a new species in the genus vesiculovirus",
abstract = "Background: Malpais Spring virus (MSPV) is a mosquito-borne rhabdovirus that infects a variety of wild and feral ungulates in New Mexico, including horses and deer. Although, initial serologic tests and electron microscopy at the time of isolation nearly 25 years ago provided evidence that MSPV is a novel virus, possibly related to vesiculoviruses, the virus still has not been approved as a new species. Findings: Use of the illumina platform allowed us to obtain the complete genome of MSPV. Analysis of the complete 11019 nt genome sequence of the prototype 85-488NM strain of MSPV indicates that it encodes the five common rhabdovirus structural proteins (N, P, M, G and L) with alternative ORFs (> 180 nt) in the N, M and G genes, including a 249 nt ORF in the G gene predicted to encode a 9.26 kDa highly basic transmembrane protein. Although antigenically very distant, phylogenetic analysis of the L gene indicates that MSPV is most closely related to Jurona virus, also isolated from mosquitoes in Brazil, as well as a number of other vesiculoviruses. Conclusions: In sum, our analysis indicates MSPV should be classified as a member of the genus Vesiculovirus, family Rhabdoviridae. The complete genome sequence of MSPV will be helpful in the development of a reverse genetics system to study the unique aspects of this vesiculovirus in vivo and in vitro, and will assist development of specific diagnostic tests to study the epidemiology of MSPV infection.",
keywords = "Family rhabdoviridae, Genome sequence, Genus vesiculovirus, Malpais spring virus (MSPV), Phylogeny",
author = "Nikos Vasilakis and Steven Widen and {Travassos Da Rosa}, {Amelia P A} and Thomas Wood and Walker, {Peter J.} and Holmes, {Edward C.} and Tesh, {Robert B.}",
year = "2014",
doi = "10.1186/1743-422X-10-69",
language = "English (US)",
volume = "10",
journal = "Virology Journal",
issn = "1743-422X",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Malpais spring virus is a new species in the genus vesiculovirus

AU - Vasilakis, Nikos

AU - Widen, Steven

AU - Travassos Da Rosa, Amelia P A

AU - Wood, Thomas

AU - Walker, Peter J.

AU - Holmes, Edward C.

AU - Tesh, Robert B.

PY - 2014

Y1 - 2014

N2 - Background: Malpais Spring virus (MSPV) is a mosquito-borne rhabdovirus that infects a variety of wild and feral ungulates in New Mexico, including horses and deer. Although, initial serologic tests and electron microscopy at the time of isolation nearly 25 years ago provided evidence that MSPV is a novel virus, possibly related to vesiculoviruses, the virus still has not been approved as a new species. Findings: Use of the illumina platform allowed us to obtain the complete genome of MSPV. Analysis of the complete 11019 nt genome sequence of the prototype 85-488NM strain of MSPV indicates that it encodes the five common rhabdovirus structural proteins (N, P, M, G and L) with alternative ORFs (> 180 nt) in the N, M and G genes, including a 249 nt ORF in the G gene predicted to encode a 9.26 kDa highly basic transmembrane protein. Although antigenically very distant, phylogenetic analysis of the L gene indicates that MSPV is most closely related to Jurona virus, also isolated from mosquitoes in Brazil, as well as a number of other vesiculoviruses. Conclusions: In sum, our analysis indicates MSPV should be classified as a member of the genus Vesiculovirus, family Rhabdoviridae. The complete genome sequence of MSPV will be helpful in the development of a reverse genetics system to study the unique aspects of this vesiculovirus in vivo and in vitro, and will assist development of specific diagnostic tests to study the epidemiology of MSPV infection.

AB - Background: Malpais Spring virus (MSPV) is a mosquito-borne rhabdovirus that infects a variety of wild and feral ungulates in New Mexico, including horses and deer. Although, initial serologic tests and electron microscopy at the time of isolation nearly 25 years ago provided evidence that MSPV is a novel virus, possibly related to vesiculoviruses, the virus still has not been approved as a new species. Findings: Use of the illumina platform allowed us to obtain the complete genome of MSPV. Analysis of the complete 11019 nt genome sequence of the prototype 85-488NM strain of MSPV indicates that it encodes the five common rhabdovirus structural proteins (N, P, M, G and L) with alternative ORFs (> 180 nt) in the N, M and G genes, including a 249 nt ORF in the G gene predicted to encode a 9.26 kDa highly basic transmembrane protein. Although antigenically very distant, phylogenetic analysis of the L gene indicates that MSPV is most closely related to Jurona virus, also isolated from mosquitoes in Brazil, as well as a number of other vesiculoviruses. Conclusions: In sum, our analysis indicates MSPV should be classified as a member of the genus Vesiculovirus, family Rhabdoviridae. The complete genome sequence of MSPV will be helpful in the development of a reverse genetics system to study the unique aspects of this vesiculovirus in vivo and in vitro, and will assist development of specific diagnostic tests to study the epidemiology of MSPV infection.

KW - Family rhabdoviridae

KW - Genome sequence

KW - Genus vesiculovirus

KW - Malpais spring virus (MSPV)

KW - Phylogeny

UR - http://www.scopus.com/inward/record.url?scp=84930615860&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84930615860&partnerID=8YFLogxK

U2 - 10.1186/1743-422X-10-69

DO - 10.1186/1743-422X-10-69

M3 - Article

AN - SCOPUS:84930615860

VL - 10

JO - Virology Journal

JF - Virology Journal

SN - 1743-422X

M1 - 69

ER -