Manganese superoxide dismutase expression is negatively associated with microRNA-301a in human pancreatic ductal adenocarcinoma

H. Pandit, W. Zhang, Y. Li, Steven Agle, X. Li, S. P. Li, G. Cui, Y. Li, R. C G Martin

Research output: Contribution to journalArticle

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Abstract

Manganese superoxide dismutase (MnSOD) expression has been found to be low in human pancreatic ductal adenocarcinoma (PDAC). Previously, we have reported that microRNA-301a (miR-301a) was found being upregulated via nuclear factor-κB (NF-κB) feedback loop in human PDAC. In this study, we investigate whether the miR-301a expression level is associated with MnSOD expression in human PDAC. We established a xenograft PDAC mouse model using transfected PanC-1 cells (miR-301a antisense or scrambled control) to investigate tumor growth and the interaction between MnSOD and miR-301a. The animal study indicated that miR-301a antisense transfection could significantly decrease the growth rate of inoculated PDAC cells, and this decrease in tumor growth rate is associated with increased MnSOD expression. To evaluate the MnSOD-miR-301a correlation in human PDAC, we have analyzed a total of 60 PDAC specimens, along with 20 normal pancreatic tissue (NPT) specimens. Human specimens confirmed a significant decrease of MnSOD expression in PDAC specimens (0.88±0.38) compared with NPT control (2.45±0.76; P

Original languageEnglish (US)
Pages (from-to)481-486
Number of pages6
JournalCancer Gene Therapy
Volume22
Issue number10
DOIs
StatePublished - Oct 1 2015
Externally publishedYes

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MicroRNAs
Superoxide Dismutase
Adenocarcinoma
Growth
Heterografts
Transfection
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Molecular Biology

Cite this

Manganese superoxide dismutase expression is negatively associated with microRNA-301a in human pancreatic ductal adenocarcinoma. / Pandit, H.; Zhang, W.; Li, Y.; Agle, Steven; Li, X.; Li, S. P.; Cui, G.; Li, Y.; Martin, R. C G.

In: Cancer Gene Therapy, Vol. 22, No. 10, 01.10.2015, p. 481-486.

Research output: Contribution to journalArticle

Pandit, H, Zhang, W, Li, Y, Agle, S, Li, X, Li, SP, Cui, G, Li, Y & Martin, RCG 2015, 'Manganese superoxide dismutase expression is negatively associated with microRNA-301a in human pancreatic ductal adenocarcinoma', Cancer Gene Therapy, vol. 22, no. 10, pp. 481-486. https://doi.org/10.1038/cgt.2015.46
Pandit, H. ; Zhang, W. ; Li, Y. ; Agle, Steven ; Li, X. ; Li, S. P. ; Cui, G. ; Li, Y. ; Martin, R. C G. / Manganese superoxide dismutase expression is negatively associated with microRNA-301a in human pancreatic ductal adenocarcinoma. In: Cancer Gene Therapy. 2015 ; Vol. 22, No. 10. pp. 481-486.
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