Manganese superoxide dismutase expression is negatively associated with microRNA-301a in human pancreatic ductal adenocarcinoma

H. Pandit, W. Zhang, Y. Li, Steven Agle, X. Li, S. P. Li, G. Cui, Y. Li, R. C G Martin

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Manganese superoxide dismutase (MnSOD) expression has been found to be low in human pancreatic ductal adenocarcinoma (PDAC). Previously, we have reported that microRNA-301a (miR-301a) was found being upregulated via nuclear factor-κB (NF-κB) feedback loop in human PDAC. In this study, we investigate whether the miR-301a expression level is associated with MnSOD expression in human PDAC. We established a xenograft PDAC mouse model using transfected PanC-1 cells (miR-301a antisense or scrambled control) to investigate tumor growth and the interaction between MnSOD and miR-301a. The animal study indicated that miR-301a antisense transfection could significantly decrease the growth rate of inoculated PDAC cells, and this decrease in tumor growth rate is associated with increased MnSOD expression. To evaluate the MnSOD-miR-301a correlation in human PDAC, we have analyzed a total of 60 PDAC specimens, along with 20 normal pancreatic tissue (NPT) specimens. Human specimens confirmed a significant decrease of MnSOD expression in PDAC specimens (0.88±0.38) compared with NPT control (2.45±0.76; P

Original languageEnglish (US)
Pages (from-to)481-486
Number of pages6
JournalCancer Gene Therapy
Volume22
Issue number10
DOIs
StatePublished - Oct 1 2015
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Molecular Biology

Cite this

Pandit, H., Zhang, W., Li, Y., Agle, S., Li, X., Li, S. P., Cui, G., Li, Y., & Martin, R. C. G. (2015). Manganese superoxide dismutase expression is negatively associated with microRNA-301a in human pancreatic ductal adenocarcinoma. Cancer Gene Therapy, 22(10), 481-486. https://doi.org/10.1038/cgt.2015.46