Mannose binding lectin (mbl2) haplotype frequencies in solid organ transplant patients and correlation with MBL protein levels - evaluation of complement-mediated effector pathway deficiency

Heather Stevenson-Lerner, Alexandra Amador, Jennifer McCue, Deborah Weppler, Panagiotis Tryphonopoulos, David Roth, Gaetano Ciancio, George Burke, Sandra Chaparro, Si Pham, Andreas Tzakis, Phillip Ruiz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mannose-binding lectin (MBL) is a protein critical in activating complement. Patients with wild-type and variant mbl2 genotypes have high or low concentrations of MBL protein, which is known to increase susceptibility to transplant rejection or infection, respectively. Our objective was to determine mbl2 genotype frequencies in future solid organ transplant recipients in order to optimize their induction and maintenance immunosuppressive therapies, and to provide MBL reference data for this unique population. We genotyped 1687 patients, and concurrently measured protein in 807 of them, during 2010-2011. Frequencies of the structural allele SNPs in our population were similar to those of other studied populations; however, Black patients with the same intermediate and deficient mbl2 genotypes as Caucasians produced significantly lower levels of MBL protein; therefore, within this population more genotypes should be considered MBL-deficient. Overall, the most critical parameter in determining serum MBL protein concentration was genotype, which was independent of other factors including ethnicity, gender, or diseased native organ type.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalTransplant Immunology
Volume28
Issue number2-3
DOIs
StatePublished - 2013

Fingerprint

Mannose-Binding Lectin
Haplotypes
Transplants
Genotype
Proteins
Population
Graft Rejection
Immunosuppressive Agents
Gene Frequency
Single Nucleotide Polymorphism
Blood Proteins
Maintenance
Infection

Keywords

  • Complement
  • Innate immunity
  • Mannose binding lectin
  • SNPs
  • Solid organ transplantation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Transplantation

Cite this

Mannose binding lectin (mbl2) haplotype frequencies in solid organ transplant patients and correlation with MBL protein levels - evaluation of complement-mediated effector pathway deficiency. / Stevenson-Lerner, Heather; Amador, Alexandra; McCue, Jennifer; Weppler, Deborah; Tryphonopoulos, Panagiotis; Roth, David; Ciancio, Gaetano; Burke, George; Chaparro, Sandra; Pham, Si; Tzakis, Andreas; Ruiz, Phillip.

In: Transplant Immunology, Vol. 28, No. 2-3, 2013, p. 73-80.

Research output: Contribution to journalArticle

Stevenson-Lerner, Heather ; Amador, Alexandra ; McCue, Jennifer ; Weppler, Deborah ; Tryphonopoulos, Panagiotis ; Roth, David ; Ciancio, Gaetano ; Burke, George ; Chaparro, Sandra ; Pham, Si ; Tzakis, Andreas ; Ruiz, Phillip. / Mannose binding lectin (mbl2) haplotype frequencies in solid organ transplant patients and correlation with MBL protein levels - evaluation of complement-mediated effector pathway deficiency. In: Transplant Immunology. 2013 ; Vol. 28, No. 2-3. pp. 73-80.
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